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Gene Review

DWF4  -  cytochrome P450 90B1

Arabidopsis thaliana

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Disease relevance of DWF4


High impact information on DWF4


Biological context of DWF4


Associations of DWF4 with chemical compounds

  • Among several triazole derivatives, brassinazole had both the highest binding affinity to DWF4 and the highest growth inhibitory activity [4].
  • These results strongly suggest that triadimefon inhibits the reaction catalysed by DWF4 protein and induces BR deficiency in plants [7].
  • In particular, the catalytic efficiency (k(cat)/K(m)) of CYP90B1 for campesterol (CR) was 325 times greater than that for CN [3].
  • Arabidopsis dwf4 was shown to be defective in a steroid 22alpha hydroxylase (CYP90B1) step that is the putative rate-limiting step in the BR biosynthetic pathway [2].

Regulatory relationships of DWF4

  • Moreover, a weak response in the mutant suggests that DWF4 alone is likely to be regulated in other way(s) in addition to BRI1 mediation [8].

Other interactions of DWF4

  • The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22alpha-hydroxylation steps in brassinosteroid biosynthesis [1].
  • However, their response to fluctuation of BR levels was highly reduced (DWF4) or nullified (the other eight genes) in a bri1 mutant [8].
  • Both show a mild phenotype in comparison with BR-deficient mutants such as cpd/cbb3, det2, and dwf4 [9].

Analytical, diagnostic and therapeutic context of DWF4

  • Sequence analysis of two other mutant alleles revealed deletions or a premature stop codon, confirming that DWF4 had been cloned [1].


  1. The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22alpha-hydroxylation steps in brassinosteroid biosynthesis. Choe, S., Dilkes, B.P., Fujioka, S., Takatsuto, S., Sakurai, A., Feldmann, K.A. Plant Cell (1998) [Pubmed]
  2. Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis. Choe, S., Fujioka, S., Noguchi, T., Takatsuto, S., Yoshida, S., Feldmann, K.A. Plant J. (2001) [Pubmed]
  3. Arabidopsis CYP90B1 catalyses the early C-22 hydroxylation of C27, C28 and C29 sterols. Fujita, S., Ohnishi, T., Watanabe, B., Yokota, T., Takatsuto, S., Fujioka, S., Yoshida, S., Sakata, K., Mizutani, M. Plant J. (2006) [Pubmed]
  4. Selective interaction of triazole derivatives with DWF4, a cytochrome P450 monooxygenase of the brassinosteroid biosynthetic pathway, correlates with brassinosteroid deficiency in planta. Asami, T., Mizutani, M., Fujioka, S., Goda, H., Min, Y.K., Shimada, Y., Nakano, T., Takatsuto, S., Matsuyama, T., Nagata, N., Sakata, K., Yoshida, S. J. Biol. Chem. (2001) [Pubmed]
  5. The regulation of DWARF4 expression is likely a critical mechanism in maintaining the homeostasis of bioactive brassinosteroids in Arabidopsis. Kim, H.B., Kwon, M., Ryu, H., Fujioka, S., Takatsuto, S., Yoshida, S., An, C.S., Lee, I., Hwang, I., Choe, S. Plant Physiol. (2006) [Pubmed]
  6. CYP724B2 and CYP90B3 Function in the Early C-22 Hydroxylation Steps of Brassinosteroid Biosynthetic Pathway in Tomato. Ohnishi, T., Watanabe, B., Sakata, K., Mizutani, M. Biosci. Biotechnol. Biochem. (2006) [Pubmed]
  7. Triadimefon, a fungicidal triazole-type P450 inhibitor, induces brassinosteroid deficiency-like phenotypes in plants and binds to DWF4 protein in the brassinosteroid biosynthesis pathway. Asami, T., Mizutani, M., Shimada, Y., Goda, H., Kitahata, N., Sekimata, K., Han, S.Y., Fujioka, S., Takatsuto, S., Sakata, K., Yoshida, S. Biochem. J. (2003) [Pubmed]
  8. Brassinosteroid homeostasis in Arabidopsis is ensured by feedback expressions of multiple genes involved in its metabolism. Tanaka, K., Asami, T., Yoshida, S., Nakamura, Y., Matsuo, T., Okamoto, S. Plant Physiol. (2005) [Pubmed]
  9. Brassinosteroid-regulated gene expression. Müssig, C., Fischer, S., Altmann, T. Plant Physiol. (2002) [Pubmed]
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