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Gene Review

DET2  -  probable steroid reductase DET2

Arabidopsis thaliana

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Disease relevance of DET2

  • The chi2 mature plants exhibited phenotypic features such as dwarfism, reduced male fertility and dark green, rounded epinastic leaves, which are characteristics of brassinosteroid-deficient mutants such as det2, cpd, and dwf4 [1].

High impact information on DET2

  • The Arabidopsis DET2 gene encodes a protein that shares significant sequence identity with mammalian steroid 5 alpha-reductases [2].
  • We show that DET2 actually acts at the second step in brassinolide biosynthesis in the 5 alpha-reduction of (24R)-24-methylcholest-4-en-3-one, which is further modified to form campestanol [3].
  • Arabidopsis det2 mutants are small dark-green dwarfs displaying pleiotropic defects in light-regulated development during multiple stages of the plant life cycle [4].
  • BR-regulated genes generally responded more potently in the det2 mutant than in the WT, and they showed only limited response in a BR-insensitive mutant, bri1 [5].
  • Both show a mild phenotype in comparison with BR-deficient mutants such as cpd/cbb3, det2, and dwf4 [6].

Biological context of DET2

  • Using a cDNA array, we identified genes that respond to BR in the det2 suspension culture of Arabidopsis, and found that epi-brassinolide upregulated transcription of the CycD3, a D-type plant cyclin gene through which cytokinin activates cell division [7].

Anatomical context of DET2


Associations of DET2 with chemical compounds

  • The results of these studies indicate the early operating steps of BR biosynthesis as: campesterol --> 4-en-3beta-ol --> 4-en-3-one --> 3-one --> campestanol in Arabidopsis, with det2 deficient in the conversion of 4-en-3-one to 3-one [9].
  • We have undertaken metabolic experiments with 2H-labeled substrates of intermediates involved in the formation of campestanol from campesterol, and quantitative analysis of intermediates in Arabidopsis wild type and det2 [9].
  • Similar results were obtained with the expression in COS-7 of recombinant AtDET2 that showed 5alphaR activity for progesterone and not for campestenone [8].

Analytical, diagnostic and therapeutic context of DET2

  • With the use of DNA microarray analysis, we identified BR-regulated genes in the wild type (WT; Columbia) of Arabidopsis and in the BR-deficient mutant, det2 [5].
  • Here, we used biochemical measurements and biological assays to determine the precise biochemical defect in det2 mutants [3].


  1. Activation of the cytochrome P450 gene, CYP72C1, reduces the levels of active brassinosteroids in vivo. Nakamura, M., Satoh, T., Tanaka, S., Mochizuki, N., Yokota, T., Nagatani, A. J. Exp. Bot. (2005) [Pubmed]
  2. A role for brassinosteroids in light-dependent development of Arabidopsis. Li, J., Nagpal, P., Vitart, V., McMorris, T.C., Chory, J. Science (1996) [Pubmed]
  3. The Arabidopsis deetiolated2 mutant is blocked early in brassinosteroid biosynthesis. Fujioka, S., Li, J., Choi, Y.H., Seto, H., Takatsuto, S., Noguchi, T., Watanabe, T., Kuriyama, H., Yokota, T., Chory, J., Sakurai, A. Plant Cell (1997) [Pubmed]
  4. Conservation of function between mammalian and plant steroid 5alpha-reductases. Li, J., Biswas, M.G., Chao, A., Russell, D.W., Chory, J. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  5. Microarray analysis of brassinosteroid-regulated genes in Arabidopsis. Goda, H., Shimada, Y., Asami, T., Fujioka, S., Yoshida, S. Plant Physiol. (2002) [Pubmed]
  6. Brassinosteroid-regulated gene expression. Müssig, C., Fischer, S., Altmann, T. Plant Physiol. (2002) [Pubmed]
  7. Promotive effect of brassinosteroids on cell division involves a distinct CycD3-induction pathway in Arabidopsis. Hu, Y., Bao, F., Li, J. Plant J. (2000) [Pubmed]
  8. 5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes. Rosati, F., Bardazzi, I., De Blasi, P., Simi, L., Scarpi, D., Guarna, A., Serio, M., Racchi, M.L., Danza, G. J. Steroid Biochem. Mol. Biol. (2005) [Pubmed]
  9. Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-En-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis. Noguchi, T., Fujioka, S., Takatsuto, S., Sakurai, A., Yoshida, S., Li, J., Chory, J. Plant Physiol. (1999) [Pubmed]
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