High impact information on FUS6

• We report here that two csn1 partially deficient Arabidopsis strains exhibit aberrant development of floral organs, decline of APETALA3 (AP3) expression, and low fertility in addition to defects in shoot and inflorescence meristems [1].
• In the COP9 complex, FUS5 may interact directly with FUS6 and COP9 [2].
• The integrated genetic and biochemical results strongly support the conclusion that both COP9 and FUS6 are components of the nuclear-localized COP9 complex [3].
• Detailed examination of the temporal morphological and cellular differentiation patterns of wild-type and mutant seedlings revealed that in darkness, seedlings homozygous for recessive mutations in COP8, COP10, and COP11 failed to suppress the photomorphogenic developmental pathway and were unable to initiate skotomorphogenesis [4].
• Arabidopsis COP8, COP10, and COP11 genes are involved in repression of photomorphogenic development in darkness [4].

Biological context of FUS6

• To study the molecular basis of the fusca phenotype, we cloned the FUS6 gene [5].
• FUS6 encodes a novel protein that is hydrophilic, alpha-helical, and contains potential protein kinase C phosphorylation sites [5].
• To distinguish CSN1-specific functions, we attempted to rescue the complex formation with deletion or point-mutation forms of CSN1 expressed as transgenes in fus6 [6].
• Using Arabidopsis Fus6 cDNA as a probe, we screened a rice root cDNA library and a rice genomic library [7].
• The mutants with light responses in the dark include some, such as det1 and cop1, that appear to identify light signal transduction components, and others, such as fus6, that may be less directly related to normal control of light responses [8].

Other interactions of FUS6

• Mutations in FUS6 and COP9 result in a shift in the electrophoretic mobility of FUS5 [2].
• Evidence for FUS6 as a component of the nuclear-localized COP9 complex in Arabidopsis [3].

Analytical, diagnostic and therapeutic context of FUS6

• Both COP9 and FUS6 cofractionated into identical high molecular mass fractions in an analytical gel filtration assay, and neither was found in its monomeric form [3].

References