Disease relevance of FPF1

• The flowering promoting factor1 ( fpf1) from Arabidopsis thaliana was transferred into Artemisia annua L. via Agrobacterium tumefaciens [1].

High impact information on FPF1

• Treatments with gibberellin (GA) and paclobutrazol, a GA biosynthesis inhibitor, as well as crosses with GA-deficient mutants indicate that FPF1 is involved in a GA-dependent signaling pathway and modulates a GA response in apical meristems during the transition to flowering [2].
• In the classical late flowering mutants, overexpression of FPF1 compensates partially for the late flowering phenotype, indicating that FPF1 acts downstream or in a parallel pathway to the mutated genes [3].
• An alpha-crystallin gene, ACD31.2 from Arabidopsis is negatively regulated by FPF1 overexpression, floral induction, gibberellins, and long days [4].
• PCR, PCR-Southern and Southern blot analyses confirmed that the foreign fpf1 gene had been integrated into the A. annua genome [1].
• FPF1 transgene leads to altered flowering time and root development in rice [5].

Biological context of FPF1

• In addition, the constitutive expression of FPF1 attenuates the severe lfy-1 phenotype under short days and phenocopies to a great extent the lfy-1 mutant grown under long-day conditions [3].
• In addition to down-regulation by FPF1 overexpression, the ACD31.2 transcript is also down-regulated by long days, floral induction, and by gibberellin in wild-type plants [4].

Analytical, diagnostic and therapeutic context of FPF1

• RT-PCR and RT-PCR-Southern analyses suggested that the foreign fpf1 gene had expressed at the transcriptional level [1].
• The presence of AtFPF1 in rice plants was confirmed by PCR, Southern and Northern blot analyses, as well as by beta-glucuronidase assay [5].

References