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Gene Review:

ADL6 - dynamin-like protein 6

Arabidopsis thaliana

Synonyms: DRP2A, DYNAMIN-RELATED PROTEIN 2A, F14N23.17, F14N23_17

Lee, S.H. et al., Jin, J.B. et al., Lam, B.C. et al., Lee, M.H. et al.

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High impact information on ADL6

In the cell, a majority of ADL6 is associated with membranes [1].
A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis [1].
Immunohistochemistry and in vivo targeting experiments revealed that ADL6 is localized to the Golgi apparatus [1].
These results suggest that ADL6 is involved in vesicle formation for vacuolar trafficking at the trans-Golgi network but not for trafficking to the plasma membrane in plant cells [1].
Here we investigate the nature of membrane binding of the Arabidopsis dynamin-like 6 (ADL6) involved in vesicle trafficking from the trans-Golgi network to the central vacuole [2].

Biological context of ADL6

Amino acid sequence analysis revealed that ADL6 has a putative pleckstrin homology (PH) domain [2].
Dynamin-related protein 2A (AtDRP2A, formally ADL6), a member of the dynamin family, is critical for protein trafficking from the TGN to the central vacuole [3].

Anatomical context of ADL6

The association of ADL6 with clathrin-coated vesicles was demonstrated by co-fractionation and immunocytochemical studies [4].

Associations of ADL6 with chemical compounds

In vitro lipid binding assays demonstrated that ADL6 showed high affinity binding to phosphatidylinositol 3-phosphate (PtdIns-3-P) and that the PH domain was responsible for this interaction [2].
Fractionation experiments by continuous sucrose gradients and gel filtration revealed that the majority of ADL6 is associated with membranes in vivo [2].

Physical interactions of ADL6

ADL6 also interacted via its C-terminus with gamma-adaptin, an adaptor protein of clathrin-coated vesicles [4].

References

A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis. Jin, J.B., Kim, Y.A., Kim, S.J., Lee, S.H., Kim, D.H., Cheong, G.W., Hwang, I. Plant Cell (2001) [Pubmed]
The intermolecular interaction between the PH domain and the C-terminal domain of Arabidopsis dynamin-like 6 determines lipid binding specificity. Lee, S.H., Jin, J.B., Song, J., Min, M.K., Park, D.S., Kim, Y.W., Hwang, I. J. Biol. Chem. (2002) [Pubmed]
A WD40 Repeat Protein, Arabidopsis Sec13 Homolog 1, May Play a Role in Vacuolar Trafficking by Controlling the Membrane Association of AtDRP2A. Lee, M.H., Lee, S.H., Kim, H., Jin, J.B., Kim, D.H., Hwang, I. Mol. Cells (2006) [Pubmed]
Regulation of ADL6 activity by its associated molecular network. Lam, B.C., Sage, T.L., Bianchi, F., Blumwald, E. Plant J. (2002) [Pubmed]

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DL3	Arabidopsis thaliana
Os02g0738900	Oryza sativa Japonica Group
Os06g0247800	Oryza sativa Japonica Group

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