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Gene Review

ORAI1  -  ORAI calcium release-activated calcium...

Homo sapiens

Synonyms: CRACM1, Calcium release-activated calcium channel protein 1, FLJ14466, IMD9, ORAT1, ...
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Disease relevance of TMEM142A


High impact information on TMEM142A


Biological context of TMEM142A

  • By site-directed mutagenesis, we show that a point mutation from glutamate to aspartate at position 180 in the conserved S1-S2 loop of Orai transforms the ion selectivity properties of CRAC current from being Ca2+-selective with inward rectification to being selective for monovalent cations and outwardly rectifying [7].
  • Recent RNA interference screens have identified several proteins that are essential for store-operated Ca2+ influx and Ca2+ release-activated Ca2+ (CRAC) channel activity in Drosophila and in mammals, including the transmembrane proteins Stim (stromal interaction molecule) and Orai [7].

Anatomical context of TMEM142A

  • Here we show that Orai1 is a plasma membrane protein, and that CRAC channel function is sensitive to mutation of two conserved acidic residues in the transmembrane segments [8].

Associations of TMEM142A with chemical compounds


Physical interactions of TMEM142A

  • Direct Ca(2+) entry into the store is regulated by its filling status in a negative and positive manner through a Ca(2+)-binding protein and Stim1/Orai complex, respectively [10].

Co-localisations of TMEM142A

  • We further demonstrate that Orai1 assembles into clusters that co-localize with STIM1 aggregations upon store depletion [11].

Regulatory relationships of TMEM142A

  • Aggregation of STIM1 underneath the plasma membrane induces clustering of Orai1 [11].
  • Single-channel cation currents present in membrane patches of TRPC3-overexpressing cells were suppressed by expression of Orai1 [12].
  • The 55-amino-acid STIM2 terminus substituted within STIM1 strikingly enhances both Orai1-mediated Ca(2+) entry and constitutive coupling to activate Orai1 channels [13].

Other interactions of TMEM142A

  • However, these experiments did not establish whether Orai is an essential intracellular link between STIM and the CRAC channel, an accessory protein in the plasma membrane, or an actual pore subunit [8].

Analytical, diagnostic and therapeutic context of TMEM142A


  1. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Feske, S., Gwack, Y., Prakriya, M., Srikanth, S., Puppel, S.H., Tanasa, B., Hogan, P.G., Lewis, R.S., Daly, M., Rao, A. Nature (2006) [Pubmed]
  2. STIM1 gates the store-operated calcium channel ORAI1 in vitro. Zhou, Y., Meraner, P., Kwon, H.T., Machnes, D., Oh-hora, M., Zimmer, J., Huang, Y., Stura, A., Rao, A., Hogan, P.G. Nat. Struct. Mol. Biol. (2010) [Pubmed]
  3. Store-operated Ca(2+) entry: a STIMulating stOrai. Taylor, C.W. Trends Biochem. Sci. (2006) [Pubmed]
  4. Dynamic Assembly of TRPC1-STIM1-Orai1 Ternary Complex Is Involved in Store-operated Calcium Influx: EVIDENCE FOR SIMILARITIES IN STORE-OPERATED AND CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL COMPONENTS. Ong, H.L., Cheng, K.T., Liu, X., Bandyopadhyay, B.C., Paria, B.C., Soboloff, J., Pani, B., Gwack, Y., Srikanth, S., Singh, B.B., Gill, D., Ambudkar, I.S. J. Biol. Chem. (2007) [Pubmed]
  5. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. Mercer, J.C., Dehaven, W.I., Smyth, J.T., Wedel, B., Boyles, R.R., Bird, G.S., Putney, J.W. J. Biol. Chem. (2006) [Pubmed]
  6. Essential role of the N-terminus of murine Orai1 in store-operated Ca(2+) entry. Takahashi, Y., Murakami, M., Watanabe, H., Hasegawa, H., Ohba, T., Munehisa, Y., Nobori, K., Ono, K., Iijima, T., Ito, H. Biochem. Biophys. Res. Commun. (2007) [Pubmed]
  7. Molecular identification of the CRAC channel by altered ion selectivity in a mutant of Orai. Yeromin, A.V., Zhang, S.L., Jiang, W., Yu, Y., Safrina, O., Cahalan, M.D. Nature (2006) [Pubmed]
  8. Orai1 is an essential pore subunit of the CRAC channel. Prakriya, M., Feske, S., Gwack, Y., Srikanth, S., Rao, A., Hogan, P.G. Nature (2006) [Pubmed]
  9. Amplification of CRAC current by STIM1 and CRACM1 (Orai1). Peinelt, C., Vig, M., Koomoa, D.L., Beck, A., Nadler, M.J., Koblan-Huberson, M., Lis, A., Fleig, A., Penner, R., Kinet, J.P. Nat. Cell Biol. (2006) [Pubmed]
  10. Calcium signaling in non-excitable cells: Ca(2+) release and influx are independent events linked to two plasma membrane Ca(2+) entry channels. Chakrabarti, R., Chakrabarti, R. J. Cell. Biochem. (2006) [Pubmed]
  11. Aggregation of STIM1 underneath the plasma membrane induces clustering of Orai1. Xu, P., Lu, J., Li, Z., Yu, X., Chen, L., Xu, T. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
  12. Orai proteins interact with TRPC channels and confer responsiveness to store depletion. Liao, Y., Erxleben, C., Yildirim, E., Abramowitz, J., Armstrong, D.L., Birnbaumer, L. Proc. Natl. Acad. Sci. U.S.A. (2007) [Pubmed]
  13. The short N-terminal domains of STIM1 and STIM2 control the activation kinetics of Orai1 channels. Zhou, Y., Mancarella, S., Wang, Y., Yue, C., Ritchie, M., Gill, D.L., Soboloff, J. J. Biol. Chem. (2009) [Pubmed]
  14. Primary prevention of osteoporosis: mass screening scenario or prescreening with questionnaires? An economic perspective. Richy, F., Ethgen, O., Bruyere, O., Mawet, A., Reginster, J.Y. J. Bone Miner. Res. (2004) [Pubmed]
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