High impact information on LRE1

• Deletion and overexpression of HLR1 causes similar but less pronounced effects compared with LRE1 [1].
• In addition, we show that increased trehalose accumulation and increased heat resistance caused by overexpression of LRE1 are also the result of inhibition of Cbk1, revealing a novel control pathway for certain targets affected by PKA [1].
• Deletion of LRE1 results in the opposite phenotypes [1].
• We have isolated the LRE1 gene as a multicopy suppressor of glucose-induced loss of heat resistance [1].
• Lre1 affects chitinase expression, trehalose accumulation and heat resistance through inhibition of the Cbk1 protein kinase in Saccharomyces cerevisiae [1].

Biological context of LRE1

• Subcloning experiments demonstrated that the production of these cell wall effects requires the presence of both LRE1 and YCL052C (PBN1), a second open reading frame present on the original plasmid [2].
• The yeast genome contains a homologue of LRE1, YDR528w, which we have called HLR1 (for homologue of Lre1) [1].

Anatomical context of LRE1

• Unexpectedly, cells carrying LRE1 at multiple copy show no alteration in cell wall glucans or glucan synthase activity [2].

Associations of LRE1 with chemical compounds

• Overexpression of LRE1 in a wild-type strain causes the same phenotype as observed in strains with reduced activity of the cAMP-PKA pathway: higher heat resistance and enhanced trehalose levels [1].

Other interactions of LRE1

• Multiple copies of PBS2, MHP1 or LRE1 produce glucanase resistance and other cell wall effects in Saccharomyces cerevisiae [2].
• The laminarinase resistance conferred by the third plasmid mapped to the previously uncharacterized YCL051W open reading frame and this gene was therefore named LRE1 (laminarinase resistance) [2].

References