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CBK1  -  Cbk1p

Saccharomyces cerevisiae S288c

Synonyms: Cell wall biosynthesis kinase, N1727, Serine/threonine-protein kinase CBK1, YNL161W
 
 
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Disease relevance of CBK1

 

High impact information on CBK1

  • We show that Cbk1 kinase and its interacting protein Mob2 regulate this asymmetry by inducing daughter-specific genetic programs [2].
  • Daughter-specific expression is due to Cbk1/Mob2-dependent activation and localization of the Ace2 transcription factor to the daughter nucleus [2].
  • Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis [3].
  • In marked contrast, phosphorylation of a C-terminal hydrophobic motif is required for Cbk1 function in vivo but not for its kinase activity, suggesting a previously unappreciated level of control for this family of kinases [3].
  • Previous work indicates that cells lacking the Dbf2p-related protein Cbk1p fail to sustain polarized growth during early bud morphogenesis and mating projection formation (Bidlingmaier, S., E.L. Weiss, C. Seidel, D.G. Drubin, and M. Snyder. 2001. Mol. Cell. Biol. 21:2449-2462) [4].
 

Biological context of CBK1

 

Anatomical context of CBK1

 

Associations of CBK1 with chemical compounds

  • The CBK1 gene, encoding a putative serine/threonine protein kinase, was identified in a screen designed to isolate mutations that affect apical growth [6].
  • Conserved serine/threonine kinase encoded by CBK1 regulates expression of several hypha-associated transcripts and genes encoding cell wall proteins in Candida albicans [8].
  • In addition, we show that increased trehalose accumulation and increased heat resistance caused by overexpression of LRE1 are also the result of inhibition of Cbk1, revealing a novel control pathway for certain targets affected by PKA [9].
 

Regulatory relationships of CBK1

  • Consistent with this, Pneumocystis CBK1 expression also stimulated transcription of the CTS1 chitinase in cbk1 Delta mutant yeast cells, an event necessary for cell wall separation [7].
  • The Saccharomyces cerevisiae Mob2p-Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell-specific localization of Ace2p transcription factor [4].
 

Other interactions of CBK1

  • CBK1 and another gene, HYM1, were previously identified in a screen for genes involved in transcriptional repression and proposed to function in the same pathway [6].
  • ACE2, CBK1, and BUD4 in budding and cell separation [10].
  • Experiments examining budding patterns and sedimentation rates both show that Ace2 and Cbk1 have independent functions in addition to their common pathway in transcription of genes such as CTS1 [10].
  • These genetic results suggest that PAG1 and CBK1 act in the same pathway [11].
  • This is most evident for yeast genes CBK1, HRR25, and SNF1 and the kinase family S6K [12].
 

Analytical, diagnostic and therapeutic context of CBK1

  • Herein, we report the identification of a cell wall biosynthesis kinase gene (CBK1) homologue in Pneumocystis carinii, isolated by differential display PCR, that is expressed optimally at physiological pH (7 to 8) as opposed to more acidic environments [7].

References

  1. Cbk1p, a protein similar to the human myotonic dystrophy kinase, is essential for normal morphogenesis in Saccharomyces cerevisiae. Racki, W.J., Bécam, A.M., Nasr, F., Herbert, C.J. EMBO J. (2000) [Pubmed]
  2. Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates. Colman-Lerner, A., Chin, T.E., Brent, R. Cell (2001) [Pubmed]
  3. Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis. Jansen, J.M., Barry, M.F., Yoo, C.K., Weiss, E.L. J. Cell Biol. (2006) [Pubmed]
  4. The Saccharomyces cerevisiae Mob2p-Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell-specific localization of Ace2p transcription factor. Weiss, E.L., Kurischko, C., Zhang, C., Shokat, K., Drubin, D.G., Luca, F.C. J. Cell Biol. (2002) [Pubmed]
  5. The Ras/protein kinase A pathway acts in parallel with the Mob2/Cbk1 pathway to effect cell cycle progression and proper bud site selection. Schneper, L., Krauss, A., Miyamoto, R., Fang, S., Broach, J.R. Eukaryotic Cell (2004) [Pubmed]
  6. The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae. Bidlingmaier, S., Weiss, E.L., Seidel, C., Drubin, D.G., Snyder, M. Mol. Cell. Biol. (2001) [Pubmed]
  7. Pneumocystis carinii cell wall biosynthesis kinase gene CBK1 is an environmentally responsive gene that complements cell wall defects of cbk-deficient yeast. Kottom, T.J., Limper, A.H. Infect. Immun. (2004) [Pubmed]
  8. Conserved serine/threonine kinase encoded by CBK1 regulates expression of several hypha-associated transcripts and genes encoding cell wall proteins in Candida albicans. McNemar, M.D., Fonzi, W.A. J. Bacteriol. (2002) [Pubmed]
  9. Lre1 affects chitinase expression, trehalose accumulation and heat resistance through inhibition of the Cbk1 protein kinase in Saccharomyces cerevisiae. Versele, M., Thevelein, J.M. Mol. Microbiol. (2001) [Pubmed]
  10. ACE2, CBK1, and BUD4 in budding and cell separation. Voth, W.P., Olsen, A.E., Sbia, M., Freedman, K.H., Stillman, D.J. Eukaryotic Cell (2005) [Pubmed]
  11. Pag1p, a novel protein associated with protein kinase Cbk1p, is required for cell morphogenesis and proliferation in Saccharomyces cerevisiae. Du, L.L., Novick, P. Mol. Biol. Cell (2002) [Pubmed]
  12. Systematic trans-genomic comparison of protein kinases between Arabidopsis and Saccharomyces cerevisiae. Wang, D., Harper, J.F., Gribskov, M. Plant Physiol. (2003) [Pubmed]
 
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