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Gene Review

BUD6  -  Bud6p

Saccharomyces cerevisiae S288c

Synonyms: AIP3, Actin-interacting protein 3, Bud site selection protein 6, L8543.5, YLR319C
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High impact information on BUD6

  • The membrane-associated protein Bud6 localized to the bud neck in a septin-dependent manner and was required to restrict the diffusion of ER membrane proteins [1].
  • Together, these results demonstrate Bud6p function in MT capture at the cell cortex, independent of Kar9p-mediated MT delivery along actin cables [2].
  • Bud6p function has been linked to Kar9p, a protein guiding MTs along actin cables [2].
  • Moreover, additive phenotypes in bud6Delta kar9Delta or bud6Delta dyn1Delta mutants underscored the separate contributions of Bud6p, Kar9p, and dynein to spindle positioning [2].
  • This program requires Bud6p/Aip3p to direct the old pole to the bud and confine the new pole to the mother cell [2].

Biological context of BUD6

  • By using the two-hybrid system, Spa2p and Bud6p were also found to interact with components of two mitogen-activated protein kinase (MAPK) pathways important for polarized cell growth [3].
  • Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites [4].
  • MATa/alpha daughter cells lacking Aip3p place their first buds normally at their distal poles but choose random sites for budding in subsequent cell cycles [4].
  • When expressed in budding yeast, spAip3p localizes similarly to Aip3p during the cell cycle and complements the cell polarity defects of an aip3Delta strain [5].
  • These differences are mediated by the relative partition of Bud6 between the bud tip and bud neck, distinguishing haploids from diploids [6].

Anatomical context of BUD6


Physical interactions of BUD6

  • Bud6p interacts with Ste11p [3].
  • We mapped Bud6 binding activity to specific residues in the carboxyl terminus of Bni1 that are adjacent to its diaphanous autoregulatory domain (DAD) [9].
  • To our knowledge, bud6p is the first protein shown to interact physically with tea1p [10].

Regulatory relationships of BUD6


Other interactions of BUD6

  • We found that Bud6 is up-regulated during bud growth, when it is delivered to the bud tip on Bni1-nucleated actin cables [9].
  • Differential activities and regulation of Saccharomyces cerevisiae formin proteins Bni1 and Bnr1 by Bud6 [9].
  • Surprisingly, the localization of Aip3p does not appear to require either polarized actin or the septin proteins of the neck filaments [4].
  • Bnr1p also directly interacted with Bud6p, a novel actin-binding protein [11].
  • Here, we show that Kar9p does not mediate Bud6p functions in spindle orientation [2].


  1. Septin-dependent compartmentalization of the endoplasmic reticulum during yeast polarized growth. Luedeke, C., Frei, S.B., Sbalzarini, I., Schwarz, H., Spang, A., Barral, Y. J. Cell Biol. (2005) [Pubmed]
  2. Differential contribution of Bud6p and Kar9p to microtubule capture and spindle orientation in S. cerevisiae. Huisman, S.M., Bales, O.A., Bertrand, M., Smeets, M.F., Reed, S.I., Segal, M. J. Cell Biol. (2004) [Pubmed]
  3. Spa2p interacts with cell polarity proteins and signaling components involved in yeast cell morphogenesis. Sheu, Y.J., Santos, B., Fortin, N., Costigan, C., Snyder, M. Mol. Cell. Biol. (1998) [Pubmed]
  4. Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites. Amberg, D.C., Zahner, J.E., Mulholland, J.W., Pringle, J.R., Botstein, D. Mol. Biol. Cell (1997) [Pubmed]
  5. Fission yeast Aip3p (spAip3p) is required for an alternative actin-directed polarity program. Jin, H., Amberg, D.C. Mol. Biol. Cell (2001) [Pubmed]
  6. Bud6 directs sequential microtubule interactions with the bud tip and bud neck during spindle morphogenesis in Saccharomyces cerevisiae. Segal, M., Bloom, K., Reed, S.I. Mol. Biol. Cell (2000) [Pubmed]
  7. Kar9p-independent microtubule capture at Bud6p cortical sites primes spindle polarity before bud emergence in Saccharomyces cerevisiae. Segal, M., Bloom, K., Reed, S.I. Mol. Biol. Cell (2002) [Pubmed]
  8. The secretory pathway mediates localization of the cell polarity regulator Aip3p/Bud6p. Jin, H., Amberg, D.C. Mol. Biol. Cell (2000) [Pubmed]
  9. Differential activities and regulation of Saccharomyces cerevisiae formin proteins Bni1 and Bnr1 by Bud6. Moseley, J.B., Goode, B.L. J. Biol. Chem. (2005) [Pubmed]
  10. Role of bud6p and tea1p in the interaction between actin and microtubules for the establishment of cell polarity in fission yeast. Glynn, J.M., Lustig, R.J., Berlin, A., Chang, F. Curr. Biol. (2001) [Pubmed]
  11. An FH domain-containing Bnr1p is a multifunctional protein interacting with a variety of cytoskeletal proteins in Saccharomyces cerevisiae. Kikyo, M., Tanaka, K., Kamei, T., Ozaki, K., Fujiwara, T., Inoue, E., Takita, Y., Ohya, Y., Takai, Y. Oncogene (1999) [Pubmed]
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