High impact information on CSR1

• Sfh2-mediated suppression of inadequate Gcs1-4 function depended on phospholipase D, whereas inadequate Gcs1-4 activity was relieved by increasing levels of diacylglycerol (DAG) [1].
• Overexpression of two Sec14p homologs Sfh2p and Sfh4p in sec14(ts) cells restored secretion and growth at the restrictive temperature but did not restore GPCho accumulation [2].
• The protein interaction of Saccharomyces cerevisiae cytoplasmic thiol peroxidase II with SFH2p and its in vivo function [3].
• The selective interaction of the dimer form of cTPx II (the oxidized form) with SFH2p was also confirmed by glutathione S-transferase pull-down and immunoprecipitation assays [3].
• Herein, we focus on mechanisms of Sfh2p and Sfh5p function in this regulatory circuit [4].

Biological context of CSR1

• SUT1 suppresses sec14-1 through upregulation of CSR1 in Saccharomyces cerevisiae [5].
• We predict that the CSR1 gene encodes a 73 kDa protein of 612 amino acids with five zinc-finger motifs at the C-terminal region [6].
• CHR1 or CSR1 on a high-copy number plasmid showed a slow-growth phenotype in a condition where the ROK1 expression is turned on from the GAL1 promoter [6].
• Beyond the new PC metabolic control features we ascribe to Sfh2p and Sfh4p we also describe a second role for Sec14p in mediating PC homeostasis [2].

Associations of CSR1 with chemical compounds

• In addition, SUT1 effects on both sec14-1 suppression and on free sterol composition were abolished in a csr1-null background, showing that this gene acts downstream of SUT1 [5].

Co-localisations of CSR1

• Consistent with these findings, Sfh2p colocalizes with PLD in endosomal compartments [7].

References