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Gene Review

RAD55  -  Rad55p

Saccharomyces cerevisiae S288c

Synonyms: D4426, DNA repair protein RAD55, YDR076W
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High impact information on RAD55

  • We observed chromosome fusions only in mutant strains expressing Rad51 and Rad55 or when Tel1 was inactivated [1].
  • By several independent criteria, RAD55- and RAD57-encoded products are shown here to exist as a stable heterodimer, with a dissociation constant of <2 x 10(-10) M [2].
  • Phosphorylation of rad55 on serines 2, 8, and 14 is required for efficient homologous recombination in the recovery of stalled replication forks [3].
  • Rad55 is a Rad51 paralog in Saccharomyces cerevisiae and functions in the assembly of the Rad51 filament, a central intermediate in recombinational DNA repair [3].
  • In contrast, rad51-K191A, a mutation that abolishes recombination and results in a protein that does not bind to single-stranded DNA (ssDNA), supports adaptation, as do Rad51 mutants impaired in interaction with Rad54p or Rad55p [4].

Biological context of RAD55


Associations of RAD55 with chemical compounds

  • RAD55 and RAD57 contain putative nucleotide binding motifs, and the importance of these motifs was determined by constructing site-directed mutations of the conserved lysine residue within the Walker A-box [9].
  • Rad55p was specifically phosphorylated in response to DNA damage induced by the alkylating agent methyl methanesulfonate, dependent on an active DNA damage checkpoint [10].
  • However, the function of Esc4 does not appear to be restricted to a Rad55-dependent process, as we observed an increase in sensitivity to the DNA alkylating agent methane methylsulfonate (MMS) in a esc4Deltarad55Delta mutant, as well as in double mutants of esc4Delta and other recombination genes, compared to the corresponding single mutants [11].

Other interactions of RAD55


  1. Recombination and the Tel1 and Mec1 checkpoints differentially effect genome rearrangements driven by telomere dysfunction in yeast. Pennaneach, V., Kolodner, R.D. Nat. Genet. (2004) [Pubmed]
  2. Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase. Sung, P. Genes Dev. (1997) [Pubmed]
  3. Phosphorylation of rad55 on serines 2, 8, and 14 is required for efficient homologous recombination in the recovery of stalled replication forks. Herzberg, K., Bashkirov, V.I., Rolfsmeier, M., Haghnazari, E., McDonald, W.H., Anderson, S., Bashkirova, E.V., Yates, J.R., Heyer, W.D. Mol. Cell. Biol. (2006) [Pubmed]
  4. Yeast Rad52 and Rad51 recombination proteins define a second pathway of DNA damage assessment in response to a single double-strand break. Lee, S.E., Pellicioli, A., Vaze, M.B., Sugawara, N., Malkova, A., Foiani, M., Haber, J.E. Mol. Cell. Biol. (2003) [Pubmed]
  5. Mutations in yeast Rad51 that partially bypass the requirement for Rad55 and Rad57 in DNA repair by increasing the stability of Rad51-DNA complexes. Fortin, G.S., Symington, L.S. EMBO J. (2002) [Pubmed]
  6. Genetic requirements for RAD51- and RAD54-independent break-induced replication repair of a chromosomal double-strand break. Signon, L., Malkova, A., Naylor, M.L., Klein, H., Haber, J.E. Mol. Cell. Biol. (2001) [Pubmed]
  7. A new recombinational DNA repair gene from Schizosaccharomyces pombe with homology to Escherichia coli RecA. Khasanov, F.K., Savchenko, G.V., Bashkirova, E.V., Korolev, V.G., Heyer, W.D., Bashkirov, V.I. Genetics (1999) [Pubmed]
  8. Characterization of null mutants of the RAD55 gene of Saccharomyces cerevisiae: effects of temperature, osmotic strength and mating type. Lovett, S.T., Mortimer, R.K. Genetics (1987) [Pubmed]
  9. Functional differences and interactions among the putative RecA homologs Rad51, Rad55, and Rad57. Johnson, R.D., Symington, L.S. Mol. Cell. Biol. (1995) [Pubmed]
  10. DNA repair protein Rad55 is a terminal substrate of the DNA damage checkpoints. Bashkirov, V.I., King, J.S., Bashkirova, E.V., Schmuckli-Maurer, J., Heyer, W.D. Mol. Cell. Biol. (2000) [Pubmed]
  11. Esc4/Rtt107 and the control of recombination during replication. Chin, J.K., Bashkirov, V.I., Heyer, W.D., Romesberg, F.E. DNA Repair (Amst.) (2006) [Pubmed]
  12. Different Mating-Type-Regulated Genes Affect the DNA Repair Defects of Saccharomyces RAD51, RAD52 and RAD55 Mutants. Valencia-Burton, M., Oki, M., Johnson, J., Seier, T.A., Kamakaka, R., Haber, J.E. Genetics (2006) [Pubmed]
  13. Direct kinase-to-kinase signaling mediated by the FHA phosphoprotein recognition domain of the Dun1 DNA damage checkpoint kinase. Bashkirov, V.I., Bashkirova, E.V., Haghnazari, E., Heyer, W.D. Mol. Cell. Biol. (2003) [Pubmed]
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