The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

RPB5  -  DNA-directed RNA polymerase core subunit RPB5

Saccharomyces cerevisiae S288c

Synonyms: ABC27, DNA-directed RNA polymerases I, II, and III 27 kDa polypeptide, DNA-directed RNA polymerases I, II, and III subunit RPABC1, RNA polymerases I, II, and III subunit ABC1, RPA7, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on RPB5

  • The Saccharomyces cerevisiae genes that encode these subunits (RPB5, RPB6, and RPB8) were isolated and sequenced, and their transcriptional start sites were deduced [1].
  • Four subunits that are common to pol I, II, and III (ABC27, ABC14.5, ABC10alpha, and ABC10beta), two that are common to pol I and III (AC40 and AC19), and one pol III-specific subunit (C11) can associate with defined regions of the two large subunits [2].
  • We demonstrate that RPB5 and the RPB1-CTD have overlapping roles in activation because the double mutant is synthetically lethal and has exacerbated activation defects at the GAL1/10 promoter [3].
  • In vivo experiments using inducible reporter plasmids and Northern analysis support the in vitro data and demonstrate that RPB5 influences activation at some, but not all, promoters [3].
  • Mutations in the C terminus of Rsc4 conferred a thermosensitive phenotype and the loss of interaction with Rpb5 [4].

Biological context of RPB5

  • The polypeptide predicted from DNA sequence of the rpb5+ gene consists of 210 amino acids with a calculated molecular weight of 23914 [5].
  • This polypeptide has a predicted pI of 9.6, matches 14 of 16 amino acids in the amino terminus of cauliflower RPB5 that was microsequenced, and shows 42 and 53% amino acid sequence identity with the yeast and human RPB5 subunits, respectively [6].
  • In addition to the already known gene RPB5, coding for a subunit shared by all three DNA directed RNA polymerases, two new open reading frames could be identified [7].
  • Here we report on the sequence and functional analysis of a 7217 bp fragment located on the right arm of chromosome II between RPB5 and CDC28 [8].

Physical interactions of RPB5

  • Rpb5 was found to interact with any fragment of Rpb1 that contained the region H, which is conserved among the subunit 1 homologues of all RNA polymerases, including the beta' subunit of prokaryotic RNA polymerases [9].
  • In agreement with the fact that Rpb5 is shared among all three forms of eukaryotic RNA polymerases, the region H of RNA polymerase I subunit 1 (Rpa190) was also found to interact with Rpb5 [9].

Other interactions of RPB5

  • Only recently has it been suggested that besides the largest subunit of RNA polymerase II, smaller subunits like Rpb3 and Rpb5 may have regulatory roles in expression of specific sets of genes [10].

Analytical, diagnostic and therapeutic context of RPB5

  • Crystallization and preliminary diffraction studies of the RNA polymerase subunit RPB5 from Saccharomyces cerevisiae [11].
  • Lethal or conditional mutants of the C-terminal globe altered the binding of Rpb5 to Rpb1-beta25/26 (prolonging the Bridge helix) and Rpb1-alpha44/47 (ahead of the Switch 1 loop and binding Rpb5 in a two-hybrid assay) [12].


  1. Subunits shared by eukaryotic nuclear RNA polymerases. Woychik, N.A., Liao, S.M., Kolodziej, P.A., Young, R.A. Genes Dev. (1990) [Pubmed]
  2. A protein-protein interaction map of yeast RNA polymerase III. Flores, A., Briand, J.F., Gadal, O., Andrau, J.C., Rubbi, L., Van Mullem, V., Boschiero, C., Goussot, M., Marck, C., Carles, C., Thuriaux, P., Sentenac, A., Werner, M. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  3. RNA polymerase subunit RPB5 plays a role in transcriptional activation. Miyao, T., Woychik, N.A. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  4. Rsc4 connects the chromatin remodeler RSC to RNA polymerases. Soutourina, J., Bordas-Le Floch, V., Gendrel, G., Flores, A., Ducrot, C., Dumay-Odelot, H., Soularue, P., Navarro, F., Cairns, B.R., Lefebvre, O., Werner, M. Mol. Cell. Biol. (2006) [Pubmed]
  5. Molecular assembly of RNA polymerase II from the fission yeast Schizosaccharomyces pombe: subunit-subunit contact network involving Rpb5. Miyao, T., Yasui, K., Sakurai, H., Yamagishi, M., Ishihama, A. Genes Cells (1996) [Pubmed]
  6. Arabidopsis thaliana RNA polymerase II subunits related to yeast and human RPB5. Larkin, R.M., Hagen, G., Guilfoyle, T.J. Gene (1999) [Pubmed]
  7. Sequence of a 4.8 kb fragment of Saccharomyces cerevisiae chromosome II including three essential open reading frames. Baur, A., Schaaff-Gerstenschläger, I., Boles, E., Miosga, T., Rose, M., Zimmermann, F.K. Yeast (1993) [Pubmed]
  8. Sequence and functional analysis of a 7.2 kb DNA fragment containing four open reading frames located between RPB5 and CDC28 on the right arm of chromosome II. Rose, M., Kiesau, P., Proft, M., Entian, K.D. Yeast (1995) [Pubmed]
  9. Mapping of Rpb3 and Rpb5 contact sites on two large subunits, Rpb1 and Rpb2, of the RNA polymerase II from fission yeast. Miyao, T., Honda, A., Qu, Z., Ishihama, A. Mol. Gen. Genet. (1998) [Pubmed]
  10. Rpb4, a non-essential subunit of core RNA polymerase II of Saccharomyces cerevisiae is important for activated transcription of a subset of genes. Pillai, B., Sampath, V., Sharma, N., Sadhale, P. J. Biol. Chem. (2001) [Pubmed]
  11. Crystallization and preliminary diffraction studies of the RNA polymerase subunit RPB5 from Saccharomyces cerevisiae. Hodach, M., Todone, F., Eloranta, J.J., Onesti, S., Weinzierl, R.O. Acta Crystallogr. D Biol. Crystallogr. (1999) [Pubmed]
  12. Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases. Zaros, C., Briand, J.F., Boulard, Y., Labarre-Mariotte, S., Garcia-Lopez, M.C., Thuriaux, P., Navarro, F. Nucleic Acids Res. (2007) [Pubmed]
WikiGenes - Universities