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Gene Review

RPS2  -  ribosomal 40S subunit protein S2

Saccharomyces cerevisiae S288c

Synonyms: 40S ribosomal protein S2, G2893, Omnipotent suppressor protein SUP44, RP12, RPS4, ...
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Disease relevance of RPS2

  • In Escherichia coli, certain alterations in S12 cause hyperaccurate translation or antibiotic resistance that can be counteracted by other changes in S5 or S4 that reduce translational accuracy [1].
  • In Arabidopsis, resistance to infection by strains of Pseudomonas syringae expressing the effector AvrRpt2 requires the plant resistance protein RPS2 [2].

High impact information on RPS2

  • Using his3 as an internal standard, the number of mRNA molecules per cell have been determined for ded1, trp3, rps4, and gall under a variety of growth conditions [3].
  • In the eukaryotic ribosome of Saccharomyces cerevisiae, mutations in SUP46 and SUP44, encoding the proteins equivalent to S4 and S5, lead to omnipotent suppression--i.e., to less accurate translation [4].
  • The SUP44 suppressor mutation occurs near a region of the protein that corresponds to the known positions of alterations in E. coli S5 ram mutations [5].
  • The five suppressors SUP35, SUP43, SUP44, SUP45 and SUP46, each mapping at a different chromosomal locus in the yeast Saccharomyces cerevisiae, suppress a wide range of mutations, including representatives of all three types of nonsense mutations, UAA, UAG and UGA [6].
  • Thus, the gene products of SUP35, SUP44, SUP45 and SUP46 are components of the 40 S subunit or are enzymes that modify the subunit [6].

Biological context of RPS2

  • These two mutants have previously been shown to exhibit a translation termination error phenotype and the sup44+ and sup46+ genes encode the yeast ribosomal proteins S4 and S9, respectively [7].
  • SUP46 and S4 are most similar in their central amino acid sequences [8].
  • However, the Lys-62-->Arg substitution decreased translational accuracy and caused antibiotic sensitivity both in nonsuppressor and in SUP44 haploids [9].
  • A gene (rps2) coding for ribosomal protein S2 (RPS2) is present in the mitochondrial (mt) genome of several monocot plants, but absent from the mtDNA of dicots [10].
  • The DNA sequencing of the cloned MNA6 gene revealed that it has an open reading frame specifying a 486 amino acid polypeptide, which appears to be a yeast mt homologue of the S4 r-protein family [11].

Associations of RPS2 with chemical compounds

  • Using a poly(U)-dependent cell-free translation system, we determined the accuracy of translation and the sensitivity to antibiotic paromomycin of yeast ribosomes carrying mutant ribosomal proteins S28 and/or S4 [9].
  • SIK2 encodes NH2-terminal acetyltransferase, and SIK3 encodes the yeast ribosomal S4 protein [12].
  • GDS1 gene was isolated as the multicopy suppressor of the glycerol-deficient phenotype caused by the nam9-1 mutation in the yeast nuclear gene encoding the mitochondrial ribosomal protein homologous to S4 proteins from various organisms [13].
  • Ribosomal protein S4 has 282 amino acids (the NH2-terminal methionine is removed after translation of the mRNA) and has a molecular weight of 31,841 [14].

Other interactions of RPS2

  • The His195 to Tyr195 missense error assay was used to demonstrate increased frequencies of missense error at codon 195 in SUP44 and SUP46 mutants [7].


  1. Alterations in ribosomal protein RPS28 can diversely affect translational accuracy in Saccharomyces cerevisiae. Anthony, R.A., Liebman, S.W. Genetics (1995) [Pubmed]
  2. Activation of a phytopathogenic bacterial effector protein by a eukaryotic cyclophilin. Coaker, G., Falick, A., Staskawicz, B. Science (2005) [Pubmed]
  3. Absolute mRNA levels and transcriptional initiation rates in Saccharomyces cerevisiae. Iyer, V., Struhl, K. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  4. An accuracy center in the ribosome conserved over 2 billion years. Alksne, L.E., Anthony, R.A., Liebman, S.W., Warner, J.R. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  5. Sequence and functional similarity between a yeast ribosomal protein and the Escherichia coli S5 ram protein. All-Robyn, J.A., Brown, N., Otaka, E., Liebman, S.W. Mol. Cell. Biol. (1990) [Pubmed]
  6. Altered 40 S ribosomal subunits in omnipotent suppressors of yeast. Eustice, D.C., Wakem, L.P., Wilhelm, J.M., Sherman, F. J. Mol. Biol. (1986) [Pubmed]
  7. Missense translation errors in Saccharomyces cerevisiae. Stansfield, I., Jones, K.M., Herbert, P., Lewendon, A., Shaw, W.V., Tuite, M.F. J. Mol. Biol. (1998) [Pubmed]
  8. The yeast omnipotent suppressor SUP46 encodes a ribosomal protein which is a functional and structural homolog of the Escherichia coli S4 ram protein. Vincent, A., Liebman, S.W. Genetics (1992) [Pubmed]
  9. Mutations in yeast ribosomal proteins S28 and S4 affect the accuracy of translation and alter the sensitivity of the ribosomes to paromomycin. Synetos, D., Frantziou, C.P., Alksne, L.E. Biochim. Biophys. Acta (1996) [Pubmed]
  10. Plant mitochondrial rps2 genes code for proteins with a C-terminal extension that is processed. Perrotta, G., Grienenberger, J.M., Gualberto, J.M. Plant Mol. Biol. (2002) [Pubmed]
  11. The single amino acid changes in the yeast mitochondrial S4 ribosomal protein cause temperature-sensitive defect in the accumulation of mitochondrial 15S rRNA. Biswas, T.K., Getz, G.S. Biochemistry (1999) [Pubmed]
  12. Genetic analysis of growth inhibition by GAL4-L kappa B-alpha in Saccharomyces cerevisiae. Morin, P.J., Downs, J.A., Snodgrass, A.M., Gilmore, T.D. Cell Growth Differ. (1995) [Pubmed]
  13. Nucleotide sequence of the GDS1 gene of Saccharomyces cerevisiae. Konopinska, A., Szczesniak, B., Boguta, M. Yeast (1995) [Pubmed]
  14. The primary structure of rat ribosomal protein S4. Devi, K.R., Chan, Y.L., Wool, I.G. Biochim. Biophys. Acta (1989) [Pubmed]
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