The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

RPB9  -  DNA-directed RNA polymerase II core...

Saccharomyces cerevisiae S288c

Synonyms: B12.6, DNA-directed RNA polymerase II 14.2 kDa polypeptide, DNA-directed RNA polymerase II subunit 9, DNA-directed RNA polymerase II subunit RPB9, RNA polymerase II subunit B9, ...
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

High impact information on RPB9

  • RPB9 appears to play a unique role in transcription initiation, as the defects revealed in its absence are distinct from those seen with mutants in RNA polymerase subunit RPB1 and factor e (TFIIB), two other yeast proteins also involved in start site selection [1].
  • Simultaneous deletion of RPB9 and RAD26 genes completely abolishes TCR in both the coding and upstream regions, indicating that no other TCR subpathway exists in RNA polymerase II-transcribed genes [2].
  • Rpb4 and Rpb9 mediate subpathways of transcription-coupled DNA repair in Saccharomyces cerevisiae [2].
  • Genes encoding homologs of the related eucaryal RNAP subunits A12.2/B12.6 and also homologs of eucaryal transcription elongation factors of the TFIIS family have been detected in Sulfolobus acidocaldarius and Thermococcus celer [3].
  • Evidence that the Transcription Elongation Function of Rpb9 Is Involved in Transcription-Coupled DNA Repair in Saccharomyces cerevisiae [4].
 

Biological context of RPB9

 

Associations of RPB9 with chemical compounds

  • Disruption of RPB9 in yeast also resulted in sensitivity to 6-azauracil, which is a phenotype linked to defects in transcription elongation [6].
 

Physical interactions of RPB9

  • In a two-hybrid test, Rpb9 physically interacts with Tfa1, the largest subunit of TFIIE [9].
 

Regulatory relationships of RPB9

  • The RPB9 subunit of RNA polymerase II regulates transcription elongation activity and is required for the action of the transcription elongation factor, TFIIS [10].
  • The Zn2 domain, which is dispensable for transcription elongation and TCR functions, is essential for Rpb9 to promote Rpb1 degradation, whereas the Zn1 and linker domains, which are essential for transcription elongation and TCR functions, play a subsidiary role in Rpb1 degradation [11].
 

Other interactions of RPB9

 

Analytical, diagnostic and therapeutic context of RPB9

  • Immunoprecipitation of RNA polymerase II from cells lacking the RPB9 gene revealed that all of the remaining 11 subunits are assembled into the enzyme, suggesting that the start site defect is attributable solely to the absence of RPB9 [1].

References

  1. RNA polymerase II subunit RPB9 is required for accurate start site selection. Hull, M.W., McKune, K., Woychik, N.A. Genes Dev. (1995) [Pubmed]
  2. Rpb4 and Rpb9 mediate subpathways of transcription-coupled DNA repair in Saccharomyces cerevisiae. Li, S., Smerdon, M.J. EMBO J. (2002) [Pubmed]
  3. Transcription in archaea: similarity to that in eucarya. Langer, D., Hain, J., Thuriaux, P., Zillig, W. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  4. Evidence that the Transcription Elongation Function of Rpb9 Is Involved in Transcription-Coupled DNA Repair in Saccharomyces cerevisiae. Li, S., Ding, B., Chen, R., Ruggiero, C., Chen, X. Mol. Cell. Biol. (2006) [Pubmed]
  5. Functional interaction between TFIIB and the Rpb9 (Ssu73) subunit of RNA polymerase II in Saccharomyces cerevisiae. Sun, Z.W., Tessmer, A., Hampsey, M. Nucleic Acids Res. (1996) [Pubmed]
  6. RNA polymerase II subunit Rpb9 regulates transcription elongation in vivo. Hemming, S.A., Jansma, D.B., Macgregor, P.F., Goryachev, A., Friesen, J.D., Edwards, A.M. J. Biol. Chem. (2000) [Pubmed]
  7. Gene RRN4 in Saccharomyces cerevisiae encodes the A12.2 subunit of RNA polymerase I and is essential only at high temperatures. Nogi, Y., Yano, R., Dodd, J., Carles, C., Nomura, M. Mol. Cell. Biol. (1993) [Pubmed]
  8. Yeast RNA polymerase II subunit RPB9 is essential for growth at temperature extremes. Woychik, N.A., Lane, W.S., Young, R.A. J. Biol. Chem. (1991) [Pubmed]
  9. The Rpb9 subunit of RNA polymerase II binds transcription factor TFIIE and interferes with the SAGA and elongator histone acetyltransferases. Van Mullem, V., Wery, M., Werner, M., Vandenhaute, J., Thuriaux, P. J. Biol. Chem. (2002) [Pubmed]
  10. Yeast RNA polymerase II subunit RPB9. Mapping of domains required for transcription elongation. Hemming, S.A., Edwards, A.M. J. Biol. Chem. (2000) [Pubmed]
  11. Yeast Rpb9 plays an important role in ubiquitylation and degradation of Rpb1 in response to UV-induced DNA damage. Chen, X., Ruggiero, C., Li, S. Mol. Cell. Biol. (2007) [Pubmed]
  12. Functional interaction between TFIIB and the Rpb2 subunit of RNA polymerase II: implications for the mechanism of transcription initiation. Chen, B.S., Hampsey, M. Mol. Cell. Biol. (2004) [Pubmed]
 
WikiGenes - Universities