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Gene Review

RAD26  -  DNA-dependent ATPase RAD26

Saccharomyces cerevisiae S288c

Synonyms: ATP-dependent helicase RAD26, DNA repair and recombination protein RAD26, GTA1085, J1606, YJR035W
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Disease relevance of RAD26


High impact information on RAD26

  • A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage [3].
  • Importantly, the simultaneous deletion of both RAD26 and RAD51 led to complete abolishment of strand-specific repair during G2/M, indicating that these genes act through two independent but complementary subpathways [4].
  • Here we show that, in haploids, the RAD26 gene is essential for the preferential repair of the TS during G1 [4].
  • Simultaneous deletion of RPB9 and RAD26 genes completely abolishes TCR in both the coding and upstream regions, indicating that no other TCR subpathway exists in RNA polymerase II-transcribed genes [5].
  • Rpb4, another non-essential subunit of RNA polymerase II, plays a dual role in regulating the two subpathways, suppressing the Rpb9 subpathway and facilitating the Rad26 subpathway [5].

Chemical compound and disease context of RAD26


Biological context of RAD26

  • These findings unmask a role of RAD26 and transcription-coupled repair in UV survival, indicate that transcription-coupled repair and global genome repair are partially overlapping, and provide evidence for a residual NER modality in the double mutants [1].
  • A strain deleted of the RAD26 gene, which is known to be required for strand-specific NER in yeast, manifested delayed recovery of mRNA synthesis, whereas a rad28 mutant, which does not show defective strand-specific repair, showed normal kinetics of recovery [6].
  • Excision repair at the level of the nucleotide in the upstream control region, the coding sequence and in the region where transcription terminates of the Saccharomyces cerevisiae MFA2 gene and the role of RAD26 [7].
  • However, the Rpb9 subpathway operates more effectively in the coding region than in the region upstream of the transcription start site, whereas the Rad26 subpathway operates equally in the two regions [5].
  • In addition, we found evidence for a transient Rad26 localization to active genes in response to DNA damage [8].

Associations of RAD26 with chemical compounds

  • Here, we examine the role of RAD26 in the repair of DNA lesions induced upon treatment with the alkylating agent methyl methanesulfonate (MMS) [9].
  • Interestingly, a synergistic increase in MMS sensitivity was observed in the rad26 Delta strain upon inactivation of NER or BER, indicating that RAD26 promotes the survival of MMS-treated cells by a mechanism that acts independently of either of these repair pathways [9].
  • Disrupting RAD26 affects nucleotide excision repair of transcribed DNA irrespective of the chromatin context, resulting in similar rates of removal for individual cyclobutane pyrimidine dimers throughout the transcribed strand [2].

Physical interactions of RAD26


Regulatory relationships of RAD26


Other interactions of RAD26


  1. Double mutants of Saccharomyces cerevisiae with alterations in global genome and transcription-coupled repair. Verhage, R.A., van Gool, A.J., de Groot, N., Hoeijmakers, J.H., van de Putte, P., Brouwer, J. Mol. Cell. Biol. (1996) [Pubmed]
  2. Rad26, the yeast homolog of the cockayne syndrome B gene product, counteracts inhibition of DNA repair due to RNA polymerase II transcription. Tijsterman, M., Brouwer, J. J. Biol. Chem. (1999) [Pubmed]
  3. A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage. Woudstra, E.C., Gilbert, C., Fellows, J., Jansen, L., Brouwer, J., Erdjument-Bromage, H., Tempst, P., Svejstrup, J.Q. Nature (2002) [Pubmed]
  4. Homologous recombination is involved in transcription-coupled repair of UV damage in Saccharomyces cerevisiae. Aboussekhra, A., Al-Sharif, I.S. EMBO J. (2005) [Pubmed]
  5. Rpb4 and Rpb9 mediate subpathways of transcription-coupled DNA repair in Saccharomyces cerevisiae. Li, S., Smerdon, M.J. EMBO J. (2002) [Pubmed]
  6. Recovery of RNA polymerase II synthesis following DNA damage in mutants of Saccharomyces cerevisiae defective in nucleotide excision repair. Reagan, M.S., Friedberg, E.C. Nucleic Acids Res. (1997) [Pubmed]
  7. Excision repair at the level of the nucleotide in the upstream control region, the coding sequence and in the region where transcription terminates of the Saccharomyces cerevisiae MFA2 gene and the role of RAD26. Teng, Y., Waters, R. Nucleic Acids Res. (2000) [Pubmed]
  8. Transcription elongation factor Spt4 mediates loss of phosphorylated RNA polymerase II transcription in response to DNA damage. Jansen, L.E., Belo, A.I., Hulsker, R., Brouwer, J. Nucleic Acids Res. (2002) [Pubmed]
  9. Yeast RAD26, a homolog of the human CSB gene, functions independently of nucleotide excision repair and base excision repair in promoting transcription through damaged bases. Lee, S.K., Yu, S.L., Prakash, L., Prakash, S. Mol. Cell. Biol. (2002) [Pubmed]
  10. Pie1, a protein interacting with Mec1, controls cell growth and checkpoint responses in Saccharomyces cerevisiae. Wakayama, T., Kondo, T., Ando, S., Matsumoto, K., Sugimoto, K. Mol. Cell. Biol. (2001) [Pubmed]
  11. Spt4 modulates Rad26 requirement in transcription-coupled nucleotide excision repair. Jansen, L.E., den Dulk, H., Brouns, R.M., de Ruijter, M., Brandsma, J.A., Brouwer, J. EMBO J. (2000) [Pubmed]
  12. Dissecting transcription-coupled and global genomic repair in the chromatin of yeast GAL1-10 genes. Li, S., Smerdon, M.J. J. Biol. Chem. (2004) [Pubmed]
  13. Repair of rDNA in Saccharomyces cerevisiae: RAD4-independent strand-specific nucleotide excision repair of RNA polymerase I transcribed genes. Verhage, R.A., Van de Putte, P., Brouwer, J. Nucleic Acids Res. (1996) [Pubmed]
  14. Transcription elongation factor S-II is not required for transcription-coupled repair in yeast. Verhage, R.A., Heyn, J., van de Putte, P., Brouwer, J. Mol. Gen. Genet. (1997) [Pubmed]
  15. LCD1: an essential gene involved in checkpoint control and regulation of the MEC1 signalling pathway in Saccharomyces cerevisiae. Rouse, J., Jackson, S.P. EMBO J. (2000) [Pubmed]
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