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Gene Review

RPT6  -  proteasome regulatory particle base...

Saccharomyces cerevisiae S288c

Synonyms: 26S protease regulatory subunit 8 homolog, CIM3, CRL3, Protein CIM3, Protein SUG1, ...
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High impact information on RPT6

  • The fusion proteins glutathione S-transferase (GST)-Rad23 and Rad4-haemagglutinin (HA), and the proteasome subunits Cim3 and Cim5, cofractionate through consecutive chromatography steps [1].
  • Mutations in SUG1 were isolated as suppressors of a mutation in the transcriptional activation domain of GAL4 [2].
  • Our observations account for the reduced ubiquitin-dependent proteolysis in sug1 mutants and suggest that the effects of sug1 mutations on transcription are indirect results of defective proteolysis [2].
  • Sug1 is a member of a large, highly conserved family of ATPases, implying a role for ATP hydrolysis in the activation of transcription [3].
  • Here, we demonstrate that proteasomal ATPases Rpt4 and Rpt6 function to connect these two histone modifications [4].

Biological context of RPT6


Associations of RPT6 with chemical compounds

  • The substituted 6-chloro-2-pyrones are slowly hydrolyzed in the presence of CRL1 and are pseudosubstrates of CRL3, but are simple reversible inhibitors of pancreatic cholesterol esterase[8]
  • Inhibition of CRL3 by substituted 6-chloro-2-pyrones was competitive with binding of the substrate p-nitrophenyl butyrate [8].
  • Molecular modeling studies suggest that the orientation of binding of these inhibitors at the active site of CRL3 can vary but that the pyrone ring consistently occupies a position close to the active site serine [8].

Physical interactions of RPT6


Regulatory relationships of RPT6

  • Surprisingly, the overall structure of the PRS2 gene was found to be identical to that of the suppressor scl1+ gene restoring the SCL1-1 mutation that suppresses crl3 cycloheximide-resistant, temperature-sensitive lethality [10].

Other interactions of RPT6

  • The 201-residue amino-terminal region of UFD4 is essential for its binding to RPT4 and RPT6 [11].
  • Sug1 and Sug2 are two of six ATPases in the 19S regulatory particle of the 26S proteasome [12].
  • Furthermore, chromatin immunoprecipitation analysis revealed the recruitment of Sug1, Sug2 and Cim5 (another of the ATPases), but not 20S proteasome core proteins, to the promoters of these genes [12].
  • SUG2 and SUG1, another 19 S component, were originally discovered as a mutation able to suppress the phenotype of a Gal4 truncation mutant (Gal4(D)p) lacking much of its AD [9].
  • In contrast, proteasome-defective rpt6/cim3 mutants accumulate polyubiquitinated Gpa1, and in this case the protein exhibits cytoplasmic localization [13].

Analytical, diagnostic and therapeutic context of RPT6


  1. Rad23 links DNA repair to the ubiquitin/proteasome pathway. Schauber, C., Chen, L., Tongaonkar, P., Vega, I., Lambertson, D., Potts, W., Madura, K. Nature (1998) [Pubmed]
  2. Identification of the gal4 suppressor Sug1 as a subunit of the yeast 26S proteasome. Rubin, D.M., Coux, O., Wefes, I., Hengartner, C., Young, R.A., Goldberg, A.L., Finley, D. Nature (1996) [Pubmed]
  3. A highly conserved ATPase protein as a mediator between acidic activation domains and the TATA-binding protein. Swaffield, J.C., Melcher, K., Johnston, S.A. Nature (1995) [Pubmed]
  4. Proteasomal ATPases link ubiquitylation of histone H2B to methylation of histone H3. Ezhkova, E., Tansey, W.P. Mol. Cell (2004) [Pubmed]
  5. The 19S regulatory particle of the proteasome is required for efficient transcription elongation by RNA polymerase II. Ferdous, A., Gonzalez, F., Sun, L., Kodadek, T., Johnston, S.A. Mol. Cell (2001) [Pubmed]
  6. Sug1 modulates yeast transcription activation by Cdc68. Xu, Q., Singer, R.A., Johnston, G.C. Mol. Cell. Biol. (1995) [Pubmed]
  7. The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth. Balzi, E., Chen, W.N., Capieaux, E., McCusker, J.H., Haber, J.E., Goffeau, A. Gene (1989) [Pubmed]
  8. Inhibition of yeast lipase (CRL1) and cholesterol esterase (CRL3) by 6-chloro-2-pyrones: comparison with porcine cholesterol esterase. Stoddard Hatch, M., Brown, W.M., Deck, J.A., Hunsaker, L.A., Deck, L.M., Vander Jagt, D.L. Biochim. Biophys. Acta (2002) [Pubmed]
  9. The Gal4 activation domain binds Sug2 protein, a proteasome component, in vivo and in vitro. Chang, C., Gonzalez, F., Rothermel, B., Sun, L., Johnston, S.A., Kodadek, T. J. Biol. Chem. (2001) [Pubmed]
  10. Molecular cloning of the yeast proteasome PRS2 gene identical to the suppressor gene scl1+. Lee, D.H., Tamura, T., Chung, C.H., Tanaka, K., Ichihara, A. Biochem. Int. (1991) [Pubmed]
  11. UFD4 lacking the proteasome-binding region catalyses ubiquitination but is impaired in proteolysis. Xie, Y., Varshavsky, A. Nat. Cell Biol. (2002) [Pubmed]
  12. The proteasomal ATPase complex is required for stress-induced transcription in yeast. Sulahian, R., Johnston, S.A., Kodadek, T. Nucleic Acids Res. (2006) [Pubmed]
  13. Differential regulation of G protein alpha subunit trafficking by mono- and polyubiquitination. Wang, Y., Marotti, L.A., Lee, M.J., Dohlman, H.G. J. Biol. Chem. (2005) [Pubmed]
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