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SPT6  -  Spt6p

Saccharomyces cerevisiae S288c

Synonyms: CRE2, Chromatin elongation factor SPT6, G6169, SSN20, Transcription elongation factor SPT6, ...
 
 
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Disease relevance of SPT6

  • Functional interaction between pleiotropic transactivator pUL69 of human cytomegalovirus and the human homolog of yeast chromatin regulatory protein SPT6 [1].
  • Furthermore, transcribed chromatin in the spt6 mutant is hypersensitive to micrococcal nuclease, and this hypersensitivity is suppressed by mutational inactivation of RNA polymerase II [2].
 

High impact information on SPT6

  • Genetic analysis has implicated SPT6, an essential gene of Saccharomyces cerevisiae, in the control of chromatin structure [3].
  • Finally, we show that Spt4 and Spt5 are tightly associated in a complex that does not contain Spt6 [4].
  • In this work, we report new genetic and biochemical studies of Spt4, Spt5, and Spt6 that reveal a role for these factors in transcription elongation [4].
  • Transcriptional activators are dispensable for transcription in the absence of Spt6-mediated chromatin reassembly of promoter regions [5].
  • Lastly, Eaf3 inhibits internal initiation within mRNA coding regions in a manner similar to FACT and Spt6 [6].
 

Biological context of SPT6

  • Therefore, having either too many or too few copies of SPT6 causes a mutant phenotype [7].
  • SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus [8].
  • In addition, we observed interesting SPT6 gene dosage effects [7].
  • SPT6 was mapped to the right arm of chromosome VII, 44 centimorgans (cM) from ADE6 and 9 cM from CLY8 [7].
  • Haploid spores carrying the spt6 null allele were inviable, indicating that the SPT6 gene is essential for mitotic growth [7].
 

Associations of SPT6 with chemical compounds

  • Also, the ability of CCR4 to activate transcription when fused to the LexA DNA binding domain was not specifically enhanced by defects in either SPT10 or SPT6 [9].
  • Recessive mutations in two negative control elements, CRE1 and CRE2, have been obtained that allow the glucose-repressible alcohol dehydrogenase (ADHII) of yeast to escape repression by glucose [10].
 

Regulatory relationships of SPT6

 

Other interactions of SPT6

  • The mechanism by which mutations in CCR4 suppress defects in SPT10 and SPT6 was examined [9].
  • Finally, the results of coimmunoprecipitation experiments demonstrate that at least the SPT5 and SPT6 proteins interact physically [12].
  • Multicopy SPT6 caused improper expression of chromosomal ADH2 [13].
  • We have now analyzed interactions between SPT4, SPT5 and SPT6 [12].
  • Finally, this study and that in the accompanying paper (L. Neigeborn, J. L. Celenza, and M. Carlson, Mol. Cell. Biol. 7:679-686, 1986) showed that spt6 and ssn20 mutations (isolated as suppressors of snf2 and snf5 [sucrose nonfermenting] mutations) identify the same gene [7].
 

Analytical, diagnostic and therapeutic context of SPT6

References

  1. Functional interaction between pleiotropic transactivator pUL69 of human cytomegalovirus and the human homolog of yeast chromatin regulatory protein SPT6. Winkler, M., aus Dem Siepen, T., Stamminger, T. J. Virol. (2000) [Pubmed]
  2. Transcription elongation factors repress transcription initiation from cryptic sites. Kaplan, C.D., Laprade, L., Winston, F. Science (2003) [Pubmed]
  3. Evidence that Spt6p controls chromatin structure by a direct interaction with histones. Bortvin, A., Winston, F. Science (1996) [Pubmed]
  4. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Hartzog, G.A., Wada, T., Handa, H., Winston, F. Genes Dev. (1998) [Pubmed]
  5. Transcriptional activators are dispensable for transcription in the absence of Spt6-mediated chromatin reassembly of promoter regions. Adkins, M.W., Tyler, J.K. Mol. Cell (2006) [Pubmed]
  6. Eaf3 chromodomain interaction with methylated H3-K36 links histone deacetylation to Pol II elongation. Joshi, A.A., Struhl, K. Mol. Cell (2005) [Pubmed]
  7. The SPT6 gene is essential for growth and is required for delta-mediated transcription in Saccharomyces cerevisiae. Clark-Adams, C.D., Winston, F. Mol. Cell. Biol. (1987) [Pubmed]
  8. SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus. Swanson, M.S., Carlson, M., Winston, F. Mol. Cell. Biol. (1990) [Pubmed]
  9. The yeast CCR4 protein is neither regulated by nor associated with the SPT6 and SPT10 proteins and forms a functionally distinct complex from that of the SNF/SWI transcription factors. Denis, C.L., Draper, M.P., Liu, H.Y., Malvar, T., Vallari, R.C., Cook, W.J. Genetics (1994) [Pubmed]
  10. Identification of new genes involved in the regulation of yeast alcohol dehydrogenase II. Denis, C.L. Genetics (1984) [Pubmed]
  11. SSN20 is an essential gene with mutant alleles that suppress defects in SUC2 transcription in Saccharomyces cerevisiae. Neigeborn, L., Celenza, J.L., Carlson, M. Mol. Cell. Biol. (1987) [Pubmed]
  12. SPT4, SPT5 and SPT6 interactions: effects on transcription and viability in Saccharomyces cerevisiae. Swanson, M.S., Winston, F. Genetics (1992) [Pubmed]
  13. Isolation and identification of genes activating UAS2-dependent ADH2 expression in Saccharomyces cerevisiae. Donoviel, M.S., Young, E.T. Genetics (1996) [Pubmed]
 
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