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Gene Review

FIP1  -  Fip1p

Saccharomyces cerevisiae S288c

Synonyms: J1911, Pre-mRNA polyadenylation factor FIP1, YJR093C
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High impact information on FIP1

  • Coimmunoprecipitation experiments reveal that RNA14, a subunit of cleavage factor I (CF I), directly interacts with FIP1, but not with PAP1 [1].
  • Recombinant FIP1 protein forms a 1:1 complex with PAP1 in vitro [1].
  • At 37 degrees C, a thermosensitive allele of FIP1 shows a shortening of poly(A) tails and a decrease in the steady-state level of actin transcripts [1].
  • The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase [1].
  • In contrast, the mutation of factors involved in the subsequent polyadenylation step (PAP1, FIP1, and YTH1) had little effect [2].

Biological context of FIP1


Associations of FIP1 with chemical compounds

  • Fip1 regulates the activity of Poly(A) polymerase through multiple interactions [6].
  • The C-terminal 789 amino acids of the Arabidopsis Fip1 protein were found to contain an RNA-binding domain; this domain correlated with an intact arginine-rich region and had a marked preference for poly(G) among the four homopolymers studied [7].

Physical interactions of FIP1

  • We demonstrate that Yth1p is a component of PF I that interacts in vivo and in vitro with Fip1p, a known PF I subunit [8].

Other interactions of FIP1

  • Yth1 was previously shown to interact with pre-mRNA and with two CPF subunits, Brr5/Ysh1 and the polyadenylation-specific Fip1, and to act in both steps of mRNA 3' end processing [9].


  1. The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase. Preker, P.J., Lingner, J., Minvielle-Sebastia, L., Keller, W. Cell (1995) [Pubmed]
  2. Coupling termination of transcription to messenger RNA maturation in yeast. Birse, C.E., Minvielle-Sebastia, L., Lee, B.A., Keller, W., Proudfoot, N.J. Science (1998) [Pubmed]
  3. Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI. Huang, M.E., Manus, V., Chuat, J.C., Galibert, F. Yeast (1996) [Pubmed]
  4. Processivity of the Saccharomyces cerevisiae poly(A) polymerase requires interactions at the carboxyl-terminal RNA binding domain. Zhelkovsky, A., Helmling, S., Moore, C. Mol. Cell. Biol. (1998) [Pubmed]
  5. Fip1 - an Essential Component of the Saccharomyces Cerevisiae Polyadenylation Machinery is Phosophorylated by Protein Kinase CK2. Zieliński, R., Hellman, U., Kubiński, K., Szyszka, R. Mol. Cell. Biochem. (2006) [Pubmed]
  6. Fip1 regulates the activity of Poly(A) polymerase through multiple interactions. Helmling, S., Zhelkovsky, A., Moore, C.L. Mol. Cell. Biol. (2001) [Pubmed]
  7. An Arabidopsis Fip1 homolog interacts with RNA and provides conceptual links with a number of other polyadenylation factor subunits. Forbes, K.P., Addepalli, B., Hunt, A.G. J. Biol. Chem. (2006) [Pubmed]
  8. The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. Barabino, S.M., Hübner, W., Jenny, A., Minvielle-Sebastia, L., Keller, W. Genes Dev. (1997) [Pubmed]
  9. Functional dissection of the zinc finger and flanking domains of the Yth1 cleavage/polyadenylation factor. Tacahashi, Y., Helmling, S., Moore, C.L. Nucleic Acids Res. (2003) [Pubmed]
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