Disease relevance of RAD7

• A fragment of the yeast DNA repair protein Rad4 confers toxicity to E. coli and is required for its interaction with Rad7 protein [1].

High impact information on RAD7

• Recently, we identified a stable subcomplex containing the yeast Rad7 and Rad16 proteins [2].
• This evidence suggests that the Sir3 protein interacts with the Rad7 protein to allow the nucleotide excision repair complex access to transcriptionally inactive chromatin [3].
• Expression of a telomeric copy of the URA3 gene was stimulated in a rad7-delta mutant, suggesting that repair of lesions in the absence of Rad7 can result in the activation of transcriptionally silenced genes [3].
• RAD7-dependent DNA repair of transcriptionally silent chromatin was shown not to induce expression of a telomeric copy of the URA3 gene, suggesting that repair of transcriptionally silent chromatin differs from transcriptionally active chromatin [3].
• The proportions of 5-FOA-resistant cells in cultures from isogenic RAD+ and rad7-delta strains containing a telomeric URA3 gene were similar, suggesting that the RAD7 gene is not involved in the production or structure of transcriptionally silent chromatin at the telomeres [3].

Biological context of RAD7

• We have used the two-hybrid system to identify proteins that interact with the product of RAD7, a gene involved in DNA repair [3].
• Transcription-independent NER of a plasmid substrate was defective in rad7, rad16, and rad23 mutant extracts [4].
• The nucleotide sequence of a 2.2-kb DNA fragment which contains the complete RAD7 gene was determined [5].
• The RAD7 and RAD16 genes, which are essential for pyrimidine dimer removal from the silent mating type loci, are also required for repair of the nontranscribed strand of an active gene in Saccharomyces cerevisiae [6].
• In the latter case, the induction of RAD7 and RAD16 may increase the turnover of complexes stalled in nontranscribed DNA so as to increase the availability of NER proteins for the repair of CPDs and EIIISS in all regions of the genome [7].

Physical interactions of RAD7

• We previously isolated a stable heterotrimeric complex of Rad7/Rad16/Abf1 from yeast which functions in the conserved global genome repair (GGR) pathway [8].

Regulatory relationships of RAD7

• In UV-irradiated JG-1 (rad1-1), rad3 and rad11 as well as in DEB-inactivated rad7, rad11, rad19 and rad20 the inability of LHR is a constitutive phenomenon and cannot be overcome by exogenous energy supply [9].

Other interactions of RAD7

• Interaction of the yeast RAD7 and SIR3 proteins: implications for DNA repair and chromatin structure [3].
• Additionally, interaction between the Rad7 and Rad16 proteins was demonstrated in vitro [4].
• Also translated regions of the COR gene RAD7 share similarities with both of the two adjacent ARC genes, ANP1 and RAD23 [10].
• Three additional genes involved in pyrimidine dimer removal in Saccharomyces cerevisiae: RAD7, RAD14 and MMS19 [11].
• This observation is of interest since, in contrast to the rad7 delta or the rad23 delta mutations, the rad1 delta mutant is very UV sensitive and highly excision defective [12].

References