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Gene Review

HRP1  -  Hrp1p

Saccharomyces cerevisiae S288c

Synonyms: CFIB, Cleavage factor IB, NAB4, NAB5, Nuclear polyadenylated RNA-binding protein 4, ...
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High impact information on HRP1

  • The protein bound directly to repeat-containing nucleoporins and to a cytosolic pool of two nuclear messenger RNA (mRNA) binding proteins, Nab2p and Nab4p [1].
  • Hmt1 methylates Yra1 and Hrp1, two mRNA-binding proteins important for mRNA processing and export [2].
  • In contrast, the mRNA cap methyltransferase and the Hrp1/CFIB polyadenylation factor cross-link to both promoter and coding regions [3].
  • The HRP1 gene was originally isolated as a suppressor of a temperature-sensitive npl3 allele, a gene encoding a protein involved in mRNA export [4].
  • Using a genetic screen in S. pombe, we identified the CHD-Mi2 class chromatin remodeling ATPase, Hrp1, as a termination factor [5].

Biological context of HRP1


Physical interactions of HRP1

  • A mutation in HRP1 that stabilizes nonsense-containing mRNAs abolishes its affinity for the DSE and fails to interact with Upf1p [10].

Regulatory relationships of HRP1

  • Mutations in HRP1 suppress all npl3 temperature-sensitive alleles but do not bypass an npl3 null allele [6].
  • Here we describe the identification and characterization of the Hrp1/Nab4 protein as a DSE-binding factor that activates NMD [10].

Other interactions of HRP1

  • The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay [10].
  • The yeast hnRNP-like protein Hrp1/Nab4 sccumulates in the cytoplasm after hyperosmotic stress: a novel Fps1-dependent response [11].
  • Supporting the hypothesis that these proteins display transcript specificity, we identified a unique 7-nucleotide sequence overrepresented in the transcripts highly associated with Nab2 and Nab4/Hrp1 using the REDUCE algorithm [7].
  • We suggest a model in which these three factors and Ufd1p are part of a regulatory complex that exploits Pab1p to link cleavage and polyadenylation factors of CFIA and CFIB (cleavage factors IA and IB) to the polyadenylation factors of CPF (cleavage and polyadenylation factor) [12].
  • Previous work demonstrated that the UV cross-linking of purified Hrp1 to RNA required the UA-rich element, but the contact point of Rna15 was not known [13].


  1. Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins. Aitchison, J.D., Blobel, G., Rout, M.P. Science (1996) [Pubmed]
  2. Arginine methyltransferase affects interactions and recruitment of mRNA processing and export factors. Yu, M.C., Bachand, F., McBride, A.E., Komili, S., Casolari, J.M., Silver, P.A. Genes Dev. (2004) [Pubmed]
  3. Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription. Komarnitsky, P., Cho, E.J., Buratowski, S. Genes Dev. (2000) [Pubmed]
  4. Hrp1, a sequence-specific RNA-binding protein that shuttles between the nucleus and the cytoplasm, is required for mRNA 3'-end formation in yeast. Kessler, M.M., Henry, M.F., Shen, E., Zhao, J., Gross, S., Silver, P.A., Moore, C.L. Genes Dev. (1997) [Pubmed]
  5. A role for chromatin remodeling in transcriptional termination by RNA polymerase II. Alén, C., Kent, N.A., Jones, H.S., O'Sullivan, J., Aranda, A., Proudfoot, N.J. Mol. Cell (2002) [Pubmed]
  6. Potential RNA binding proteins in Saccharomyces cerevisiae identified as suppressors of temperature-sensitive mutations in NPL3. Henry, M., Borland, C.Z., Bossie, M., Silver, P.A. Genetics (1996) [Pubmed]
  7. Functional specificity of shuttling hnRNPs revealed by genome-wide analysis of their RNA binding profiles. Kim Guisbert, K., Duncan, K., Li, H., Guthrie, C. RNA (2005) [Pubmed]
  8. Kap104p-mediated nuclear import. Nuclear localization signals in mRNA-binding proteins and the role of Ran and Rna. Lee, D.C., Aitchison, J.D. J. Biol. Chem. (1999) [Pubmed]
  9. A specific RNA-protein interaction at yeast polyadenylation efficiency elements. Chen, S., Hyman, L.E. Nucleic Acids Res. (1998) [Pubmed]
  10. The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay. González, C.I., Ruiz-Echevarría, M.J., Vasudevan, S., Henry, M.F., Peltz, S.W. Mol. Cell (2000) [Pubmed]
  11. The yeast hnRNP-like protein Hrp1/Nab4 sccumulates in the cytoplasm after hyperosmotic stress: a novel Fps1-dependent response. Henry, M.F., Mandel, D., Routson, V., Henry, P.A. Mol. Biol. Cell (2003) [Pubmed]
  12. Identification of factors regulating poly(A) tail synthesis and maturation. Mangus, D.A., Smith, M.M., McSweeney, J.M., Jacobson, A. Mol. Cell. Biol. (2004) [Pubmed]
  13. Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation. Gross, S., Moore, C.L. Mol. Cell. Biol. (2001) [Pubmed]
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