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Gene Review

CYC2  -  Cyc2p

Saccharomyces cerevisiae S288c

Synonyms: Cytochrome c assembly flavoprotein CYC2, Cytochrome c mitochondrial import factor CYC2, OR26.30, YOR037W
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High impact information on CYC2


Biological context of CYC2

  • Both CYC2 and CYC3 are single-copy genes and expressed in all life cycle stages of the parasite [3].
  • To determine if CYC2 is found in a complex with previously identified trypanosome cdc2-related kinases (CRKs), the CYC2 gene was fused to the TY epitope tag, integrated into the trypanosome genome, and expressed under inducible control [3].
  • Gene knockout experiments provide evidence that CYC2 is an essential gene, and co-immune precipitations together with a two-hybrid interaction assay demonstrated that CYC2 interacts with CRK3 [3].
  • We show that Cyc2p is required for the activity of CCHL toward apocytochrome c and c1 and becomes essential for the heme attachment to apocytochrome c1 carrying a CAPCH instead of CAACH heme binding site [4].

Associations of CYC2 with chemical compounds

  • Based on the functional interaction of Cyc2p with CCHL and the presence of a putative FAD-binding site in the protein, we hypothesize that Cyc2p controls the redox chemistry of the heme lyase reaction [5].

Physical interactions of CYC2

  • We postulate that, in vivo, Cyc2p interacts with CCHL and is involved in the reduction of heme prior to its ligation to apocytochrome c by CCHL [4].

Other interactions of CYC2

  • CYC2 encodes a 24-kDa protein that has sequence identity to the Neurospora crassa PREG1 and the S. cerevisiae PHO80 cyclin [3].
  • None of the cyc strains surveyed (cyc1, cyc2, cyc3, cyc4, cyc5, and cyc6) had diminished levels of methylase, although some of them were completely or almost completely deficient in cytochrome c [6].
  • Requirements of Cyc2p and the porin, Por1p, for ionic stability and mitochondrial integrity in Saccharomyces cerevisiae [7].


  1. PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Gust, B., Challis, G.L., Fowler, K., Kieser, T., Chater, K.F. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  2. CYC2 encodes a factor involved in mitochondrial import of yeast cytochrome c. Dumont, M.E., Schlichter, J.B., Cardillo, T.S., Hayes, M.K., Bethlendy, G., Sherman, F. Mol. Cell. Biol. (1993) [Pubmed]
  3. Isolation of Trypanosoma brucei CYC2 and CYC3 cyclin genes by rescue of a yeast G(1) cyclin mutant. Functional characterization of CYC2. Van Hellemond, J.J., Neuville, P., Schwarz, R.T., Matthews, K.R., Mottram, J.C. J. Biol. Chem. (2000) [Pubmed]
  4. Cyc2p, a membrane-bound flavoprotein involved in the maturation of mitochondrial c-type cytochromes. Bernard, D.G., Quevillon-Cheruel, S., Merchant, S., Guiard, B., Hamel, P.P. J. Biol. Chem. (2005) [Pubmed]
  5. Overlapping specificities of the mitochondrial cytochrome c and c1 heme lyases. Bernard, D.G., Gabilly, S.T., Dujardin, G., Merchant, S., Hamel, P.P. J. Biol. Chem. (2003) [Pubmed]
  6. Yeast cytochrome c-specific protein-lysine methyltransferase: coordinate regulation with cytochrome c and activities in cyc mutants. Liao, H.N., Sherman, F. J. Bacteriol. (1979) [Pubmed]
  7. Requirements of Cyc2p and the porin, Por1p, for ionic stability and mitochondrial integrity in Saccharomyces cerevisiae. Sánchez, N.S., Pearce, D.A., Cardillo, T.S., Uribe, S., Sherman, F. Arch. Biochem. Biophys. (2001) [Pubmed]
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