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Gene Review

MRS2  -  Mrs2p

Saccharomyces cerevisiae S288c

Synonyms: Magnesium transporter MRS2, mitochondrial, RNA-splicing protein MRS2, YOR334W
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High impact information on MRS2

  • Here we show that mutant alleles of the MRS2 gene as well as overexpression of this gene both increase intramitochondrial Mg(2+) concentrations and compensate for splicing defects of group II introns in mit(-) mutants M1301 and B-loop [1].
  • Yet, covariation of Mg(2+) concentrations and splicing is similarly seen when some other genes affecting mitochondrial Mg(2+) concentrations are overexpressed in an mrs2Delta mutant, indicating that not the Mrs2 protein per se but certain Mg(2+) concentrations are essential for group II intron splicing [1].
  • The product of the nuclear MRS2 gene, Mrs2p, is the only candidate splicing factor essential for all group II introns in mitochondria of the yeast Saccharomyces cerevisiae [1].
  • Cross-linking experiments revealed the presence of Mrs2p-containing complexes in the mitochondrial membrane, probably constituting Mrs2p homo- oligomers [2].
  • While its structural and functional similarity to the bacterial Mg(2+) transport protein CorA suggested a role for Mrs2p in Mg(2+) influx into the organelle, other functions in cation homeostasis could not be excluded [2].

Biological context of MRS2

  • This supports the notion that the MRS2 gene product is bifunctional i.e., it is essential for the splicing of group II introns and is also involved in processes of mitochondrial biogenesis other than RNA splicing [3].
  • In strains devoid of any mitochondrial introns the MRS2 gene disruption still causes a pet- phenotype and cytochrome deficiency, although the standard pattern of mitochondrial translation products is produced [4].
  • In this study we report on the isolation, nucleotide sequence, and possible functions of the nuclear MRS2 gene [4].
  • When present on high copy number plasmids, the MRS2 gene acts as a suppressor of various mitochondrial intron mutations, suggesting that the MRS2 protein functions as a splicing factor [4].
  • Here we cloned and sequenced the suppressor allele of one such gene, and found it to be a missense mutation of the MRS2 gene (MRS2-L232F) [5].

Anatomical context of MRS2


Other interactions of MRS2

  • The PIF1 and MRS2 gene products have previously been shown to be essential for mitochondrial DNA maintenance at elevated temperatures and mitochondrial group II intron splicing, respectively, in the yeast Saccharomyces cerevisiae [8].
  • The yeast ORF YPL060w/LPE10 encodes a homologue of the mitochondrial protein Mrs2p [9].
  • Alr1-type proteins are distant relatives of the mitochondrial and bacterial Mg(2+)-transport proteins, Mrs2p and CorA, respectively, with which they have two adjacent TM domains and a short Mg(2+) signature motif in common [10].
  • Here we show that both Mrs2 and Yta10 transiently accumulate as sorting intermediates in the matrix before they integrate into the inner membrane [11].


  1. Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo. Gregan, J., Kolisek, M., Schweyen, R.J. Genes Dev. (2001) [Pubmed]
  2. Mrs2p is an essential component of the major electrophoretic Mg2+ influx system in mitochondria. Kolisek, M., Zsurka, G., Samaj, J., Weghuber, J., Schweyen, R.J., Schweigel, M. EMBO J. (2003) [Pubmed]
  3. A multitude of suppressors of group II intron-splicing defects in yeast. Waldherr, M., Ragnini, A., Jank, B., Teply, R., Wiesenberger, G., Schweyen, R.J. Curr. Genet. (1993) [Pubmed]
  4. The nuclear gene MRS2 is essential for the excision of group II introns from yeast mitochondrial transcripts in vivo. Wiesenberger, G., Waldherr, M., Schweyen, R.J. J. Biol. Chem. (1992) [Pubmed]
  5. Mutant alleles of the MRS2 gene of yeast nuclear DNA suppress mutations in the catalytic core of a mitochondrial group II intron. Schmidt, U., Maue, I., Lehmann, K., Belcher, S.M., Stahl, U., Perlman, P.S. J. Mol. Biol. (1998) [Pubmed]
  6. Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae. Weghuber, J., Dieterich, F., Froschauer, E.M., Svidovà, S., Schweyen, R.J. FEBS J. (2006) [Pubmed]
  7. The bacterial magnesium transporter CorA can functionally substitute for its putative homologue Mrs2p in the yeast inner mitochondrial membrane. Bui, D.M., Gregan, J., Jarosch, E., Ragnini, A., Schweyen, R.J. J. Biol. Chem. (1999) [Pubmed]
  8. Overexpression of a novel member of the mitochondrial carrier family rescues defects in both DNA and RNA metabolism in yeast mitochondria. Van Dyck, E., Jank, B., Ragnini, A., Schweyen, R.J., Duyckaerts, C., Sluse, F., Foury, F. Mol. Gen. Genet. (1995) [Pubmed]
  9. The mitochondrial inner membrane protein Lpe10p, a homologue of Mrs2p, is essential for magnesium homeostasis and group II intron splicing in yeast. Gregan, J., Bui, D.M., Pillich, R., Fink, M., Zsurka, G., Schweyen, R.J. Mol. Gen. Genet. (2001) [Pubmed]
  10. Oligomerization of the Mg(2+)-transport proteins Alr1p and Alr2p in yeast plasma membrane. Wachek, M., Aichinger, M.C., Stadler, J.A., Schweyen, R.J., Graschopf, A. FEBS J. (2006) [Pubmed]
  11. Insertion of bitopic membrane proteins into the inner membrane of mitochondria involves an export step from the matrix. Baumann, F., Neupert, W., Herrmann, J.M. J. Biol. Chem. (2002) [Pubmed]
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