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Gene Review

ERG5  -  C-22 sterol desaturase

Saccharomyces cerevisiae S288c

Synonyms: CYP61, Cytochrome P450 61, YM9711.02C, YMR015C
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High impact information on ERG5

  • Purification of the cytosolic mutant CPR indicated properties identical to native CPR and an ability to reconstitute ergosterol biosynthesis when added to a cell-free system, as well as to allow reconstitution of activity with purified CYP61, sterol 22-desaturase [1].
  • These and Ki determination for fluconazole (0.14 nM) revealed CYP61 to have a similar affinity to azole drugs when compared with data available for CYP51, and the implications for antifungal treatment were considered [2].
  • Characterization of Saccharomyces cerevisiae CYP61, sterol delta22-desaturase, and inhibition by azole antifungal agents [2].
  • Cytochrome P-45061 (CYP61) was a cytochrome P-450 revealed during the yeast genome project when chromosome XIII was sequenced [2].
  • The enzyme kinetics associated with its endogenous role in sterol Delta22-desaturation revealed a Km of 20.4 microM and a Vmax of 2.9nmol/min/nmol CYP61 [2].

Biological context of ERG5


Associations of ERG5 with chemical compounds

  • The ERG5 gene from Saccharomyces cerevisiae was cloned by complementation of an erg5-1 mutation using a negative selection protocol involving screening for nystatin-sensitive transformants [3].
  • Nine different incubations gave altogether 16 sterol metabolites, including seven delta(22E) sterols formed by action of the yeast C-22 desaturase (Erg5p) [6].


  1. The N-terminal membrane domain of yeast NADPH-cytochrome P450 (CYP) oxidoreductase is not required for catalytic activity in sterol biosynthesis or in reconstitution of CYP activity. Venkateswarlu, K., Lamb, D.C., Kelly, D.E., Manning, N.J., Kelly, S.L. J. Biol. Chem. (1998) [Pubmed]
  2. Characterization of Saccharomyces cerevisiae CYP61, sterol delta22-desaturase, and inhibition by azole antifungal agents. Kelly, S.L., Lamb, D.C., Baldwin, B.C., Corran, A.J., Kelly, D.E. J. Biol. Chem. (1997) [Pubmed]
  3. Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis. Skaggs, B.A., Alexander, J.F., Pierson, C.A., Schweitzer, K.S., Chun, K.T., Koegel, C., Barbuch, R., Bard, M. Gene (1996) [Pubmed]
  4. Sterol biosynthesis by symbiotes: cytochrome P450 sterol C-22 desaturase genes from yeastlike symbiotes of rice planthoppers and anobiid beetles. Noda, H., Koizumi, Y. Insect Biochem. Mol. Biol. (2003) [Pubmed]
  5. Purification, reconstitution, and inhibition of cytochrome P-450 sterol delta22-desaturase from the pathogenic fungus Candida glabrata. Lamb, D.C., Maspahy, S., Kelly, D.E., Manning, N.J., Geber, A., Bennett, J.E., Kelly, S.L. Antimicrob. Agents Chemother. (1999) [Pubmed]
  6. Alternative pathways of sterol synthesis in yeast. Use of C(27) sterol tracers to study aberrant double-bond migrations and evaluate their relative importance. Ruan, B., Lai, P.S., Yeh, C.W., Wilson, W.K., Pang, J., Xu, R., Matsuda, S.P., Schroepfer, G.J. Steroids (2002) [Pubmed]
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