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Chemical Compound Review

ARONIS24603     20-[(3S,4S,5S,6R)-4-amino- 3,5-dihydroxy-6...

Synonyms: AC1L9AIN, AKOS005111369, MLS003922079, SMR002546705, NCGC00247769-01, ...
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Disease relevance of nystatin


Psychiatry related information on nystatin


High impact information on nystatin

  • The AMBr cell line also exhibited increased resistance to other polyene macrolide antibiotics such as nystatin and pentamycin [8].
  • We show that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment [9].
  • The ouabain- and bumetanide-resistant K efflux from CC cells was volume and pH dependent: K efflux from CC cells rose from 5-6 to 20-25 mmol/liter of cells X h, when cell volume was increased by increasing cell solute content (nystatin method) or by exposure to hypotonic media [10].
  • Cellular respiration was measured upon addition of nystatin (Nys) to maximally stimulate Na pump activity [4].
  • This sensitivity was partially corrected by rehydrating xerocytic erythrocytes by means of the cation-ionophore nystatin in a high potassium buffer [11].

Chemical compound and disease context of nystatin


Biological context of nystatin

  • Cyanohydrin acetonide alkylations were used to assemble the carbon skeleton, and a simple modification of the strategy allowed an advanced intermediate to be converted to either the candidin polyol or the nystatin polyol [17].
  • The action was concentration dependent, with an IC50 of 0.7 +/- 0.4 nmol/L (n = 22), and was also observed using the nystatin method [18].
  • Stimulation of the (Na+,K+)-ATPase produced as a result of nystatin-mediated dissipation of plasma membrane Na+ and K+ gradients results in increased respiration with an oxygen consumption rate characteristic of optimal ATP synthesis (state 3) [19].
  • PKC sequestration and pericentrion formation were blocked by hypertonic sucrose as well as by potassium depletion (inhibitors of clathrin-dependent endocytosis) but not by nystatin or filipin, which inhibit clathrin-independent pathways [20].
  • Decreasing [Cl-]i (without concomitant cell shrinkage) by incubating cultures with apical nystatin and reduced apical [Cl-] ([Cl-]alpha) likewise increases both cotransport activity and cotransport protein phosphorylation [21].

Anatomical context of nystatin

  • Selective permeabilization of apical or basolateral membranes with the monovalent ionophore nystatin was used to isolate basolateral K+ and apical Cl- channel activity, respectively [22].
  • METHODS: Guinea pig colon epithelium with the apical membrane perforated with nystatin in Ussing chambers is used to show a basolateral Cl(-) conductance [23].
  • Apical addition of nystatin virtually abolished amiloride-sensitive apical sodium uptake in both groups, indicating that the ionophore formed channels in the apical membrane of noninflamed and diseased mucosa [24].
  • Further, functional studies indicated that the localization of uPAR and its ligand in caveolae enhances pericellular plasminogen activation, since treatment of the cells with drugs that interfere with the structural integrity of caveolae, such as nystatin, markedly reduced cell surface plasmin generation [25].
  • Here, we show that the GPI-anchored CD59 molecule on Jurkat T cells is internalized after cross-linking, a process inhibited by nystatin, a sterol chelating agent [26].

Associations of nystatin with other chemical compounds


Gene context of nystatin

  • Lipid raft integrity is essential for LPS-cellular activation, since raft-disrupting drugs, such as nystatin or MCD, inhibit LPS-induced TNF-alpha secretion [31].
  • The inhibitory effect of EGF on Na(+) absorption was not due to inhibition of basolateral Na(+)-K(+)-ATPase, because the pump current elicited by permeabilization of apical membrane with nystatin was not reduced by EGF [32].
  • Hence, a TLR-dependent mechanism could serve as the molecular basis for the proinflammatory properties of nystatin [33].
  • A ras1 strain transformed with this plasmid was subjected to ethyl methanesulfonate mutagenesis and nystatin enrichment [34].
  • The ERG5 gene from Saccharomyces cerevisiae was cloned by complementation of an erg5-1 mutation using a negative selection protocol involving screening for nystatin-sensitive transformants [35].

Analytical, diagnostic and therapeutic context of nystatin

  • Membrane voltage (Vm) and ion currents of pod were examined with the patch clamp technique in fast whole cell and whole cell nystatin configuration [36].
  • Uphill adenosine transport was not observed when extracellular Na+ was replaced by Li+, K+, Cs+, or N-methyl-D-glucammonium ions, or after treatment of the cells with nystatin, a Na+ ionophore [37].
  • Site-specific mutagenesis of Ser-170 both in the wild-type NysA and in the hybrid loading module containing malonyl-specific acyltransferase domain from the extender module had no effect on nystatin biosynthesis [38].
  • The changes of volume distribution curves of erythrocytes during and after lysis by complement or nystatin or in hypotonic buffers were measured by flow cytometry [39].
  • 6. Use of nystatin permitted assessment of whether microelectrode impalement introduced a significant shunt conductance into the untreated apical membrane, with the conclusion that such a shunt was negligible in the present experiments [40].


  1. A randomized, double-blind trial of nystatin therapy for the candidiasis hypersensitivity syndrome. Dismukes, W.E., Wade, J.S., Lee, J.Y., Dockery, B.K., Hain, J.D. N. Engl. J. Med. (1990) [Pubmed]
  2. Nystatin prophylaxis in leukemia and lymphoma. Williams, C.J. N. Engl. J. Med. (1978) [Pubmed]
  3. A comparison of trimethoprim-sulfamethoxazole plus nystatin with gentamicin plus nystatin in the prevention of infections in acute leukemia. Wade, J.C., Schimpff, S.C., Hargadon, M.T., Fortner, C.L., Young, V.M., Wiernik, P.H. N. Engl. J. Med. (1981) [Pubmed]
  4. Intracellular respiratory dysfunction and cell injury in short-term anoxia of rabbit renal proximal tubules. Takano, T., Soltoff, S.P., Murdaugh, S., Mandel, L.J. J. Clin. Invest. (1985) [Pubmed]
  5. Disassembly of viral membranes by complement independent of channel formation. Esser, A.F., Bartholomew, R.M., Jensen, F.C., Müller-Eberhard, H.J. Proc. Natl. Acad. Sci. U.S.A. (1979) [Pubmed]
  6. A population-based case-control teratological study of oral nystatin treatment during pregnancy. Czeizel, A.E., Kazy, Z., Puhó, E. Scand. J. Infect. Dis. (2003) [Pubmed]
  7. Imidazoles for vaginitis. Lee, C.R., McKenzie, C.A., Nobles, A. American pharmacy. (1991) [Pubmed]
  8. Isolation and characterization of amphotericin B-resistant cell lines in Chinese hamster cells. Hidaka, K., Endo, H., Akiyama, S., Kuwano, M. Cell (1978) [Pubmed]
  9. Functional profiling of the Saccharomyces cerevisiae genome. Giaever, G., Chu, A.M., Ni, L., Connelly, C., Riles, L., Véronneau, S., Dow, S., Lucau-Danila, A., Anderson, K., André, B., Arkin, A.P., Astromoff, A., El-Bakkoury, M., Bangham, R., Benito, R., Brachat, S., Campanaro, S., Curtiss, M., Davis, K., Deutschbauer, A., Entian, K.D., Flaherty, P., Foury, F., Garfinkel, D.J., Gerstein, M., Gotte, D., Güldener, U., Hegemann, J.H., Hempel, S., Herman, Z., Jaramillo, D.F., Kelly, D.E., Kelly, S.L., Kötter, P., LaBonte, D., Lamb, D.C., Lan, N., Liang, H., Liao, H., Liu, L., Luo, C., Lussier, M., Mao, R., Menard, P., Ooi, S.L., Revuelta, J.L., Roberts, C.J., Rose, M., Ross-Macdonald, P., Scherens, B., Schimmack, G., Shafer, B., Shoemaker, D.D., Sookhai-Mahadeo, S., Storms, R.K., Strathern, J.N., Valle, G., Voet, M., Volckaert, G., Wang, C.Y., Ward, T.R., Wilhelmy, J., Winzeler, E.A., Yang, Y., Yen, G., Youngman, E., Yu, K., Bussey, H., Boeke, J.D., Snyder, M., Philippsen, P., Davis, R.W., Johnston, M. Nature (2002) [Pubmed]
  10. Regulation of cation content and cell volume in hemoglobin erythrocytes from patients with homozygous hemoglobin C disease. Brugnara, C., Kopin, A.S., Bunn, H.F., Tosteson, D.C. J. Clin. Invest. (1985) [Pubmed]
  11. Exercise-induced hemolysis in xerocytosis. Erythrocyte dehydration and shear sensitivity. Platt, O.S., Lux, S.E., Nathan, D.G. J. Clin. Invest. (1981) [Pubmed]
  12. A comparative trial of clotrimazole troches and oral nystatin suspension in recipients of renal transplants. Use in prophylaxis of oropharyngeal candidiasis. Gombert, M.E., duBouchet, L., Aulicino, T.M., Butt, K.M. JAMA (1987) [Pubmed]
  13. Efficacy of ketoconazole v nystatin in prevention of fungal infections in neutropenic patients. Jones, P.G., Kauffman, C.A., McAuliffe, L.S., Liepman, M.K., Bergman, A.G. Arch. Intern. Med. (1984) [Pubmed]
  14. Treatment of oral candidiasis: a study to determine the clinical response of sodium benzoate compared with nystatin suspension. Moshi, A.H., Jorgensen, A.F., Pallangyo, K. AIDS (1998) [Pubmed]
  15. Evaluating diagnosis and treatment of oral and esophageal candidiasis in Ugandan AIDS patients. Ravera, M., Reggiori, A., Agliata, A.M., Rocco, R.P. Emerging Infect. Dis. (1999) [Pubmed]
  16. Influenza virus can enter and infect cells in the absence of clathrin-mediated endocytosis. Sieczkarski, S.B., Whittaker, G.R. J. Virol. (2002) [Pubmed]
  17. A unified approach to polyene macrolides: synthesis of candidin and nystatin polyols. Kadota, I., Hu, Y., Packard, G.K., Rychnovsky, S.D. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  18. Endothelin-1 inhibits the slow component of cardiac delayed rectifier K+ currents via a pertussis toxin-sensitive mechanism. Washizuka, T., Horie, M., Watanuki, M., Sasayama, S. Circ. Res. (1997) [Pubmed]
  19. Mitochondrial respiratory capacity and Na+- and K+-dependent adenosine triphosphatase-mediated ion transport in the intact renal cell. Harris, S.I., Balaban, R.S., Barrett, L., Mandel, L.J. J. Biol. Chem. (1981) [Pubmed]
  20. Dynamic sequestration of the recycling compartment by classical protein kinase C. Idkowiak-Baldys, J., Becker, K.P., Kitatani, K., Hannun, Y.A. J. Biol. Chem. (2006) [Pubmed]
  21. [Cl-]i-dependent phosphorylation of the Na-K-Cl cotransport protein of dog tracheal epithelial cells. Haas, M., McBrayer, D., Lytle, C. J. Biol. Chem. (1995) [Pubmed]
  22. Levamisole inhibits intestinal Cl- secretion via basolateral K+ channel blockade. Mun, E.C., Mayol, J.M., Riegler, M., O'Brien, T.C., Farokhzad, O.C., Song, J.C., Pothoulakis, C., Hrnjez, B.J., Matthews, J.B. Gastroenterology (1998) [Pubmed]
  23. Basolateral ClC-2 chloride channels in surface colon epithelium: regulation by a direct effect of intracellular chloride. Catalán, M., Niemeyer, M.I., Cid, L.P., Sepúlveda, F.V. Gastroenterology (2004) [Pubmed]
  24. Cellular basis for defective electrolyte transport in inflamed human colon. Sandle, G.I., Higgs, N., Crowe, P., Marsh, M.N., Venkatesan, S., Peters, T.J. Gastroenterology (1990) [Pubmed]
  25. The urokinase-type plasminogen activator receptor, a GPI-linked protein, is localized in caveolae. Stahl, A., Mueller, B.M. J. Cell Biol. (1995) [Pubmed]
  26. Endocytosis of GPI-anchored proteins in human lymphocytes: role of glycolipid-based domains, actin cytoskeleton, and protein kinases. Deckert, M., Ticchioni, M., Bernard, A. J. Cell Biol. (1996) [Pubmed]
  27. Inactivation of Ca conductance dependent on entry of Ca ions in molluscan neurons. Tillotson, D. Proc. Natl. Acad. Sci. U.S.A. (1979) [Pubmed]
  28. Involvement of cell calcium and transmembrane potential in control of hepatocyte volume. Khalbuss, W.E., Wondergem, R. Hepatology (1991) [Pubmed]
  29. Cisplatin-induced CD95 redistribution into membrane lipid rafts of HT29 human colon cancer cells. Lacour, S., Hammann, A., Grazide, S., Lagadic-Gossmann, D., Athias, A., Sergent, O., Laurent, G., Gambert, P., Solary, E., Dimanche-Boitrel, M.T. Cancer Res. (2004) [Pubmed]
  30. Intracellular Na+ controls cell surface expression of Na,K-ATPase via a cAMP-independent PKA pathway in mammalian kidney collecting duct cells. Vinciguerra, M., Deschênes, G., Hasler, U., Mordasini, D., Rousselot, M., Doucet, A., Vandewalle, A., Martin, P.Y., Féraille, E. Mol. Biol. Cell (2003) [Pubmed]
  31. Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation. Triantafilou, M., Miyake, K., Golenbock, D.T., Triantafilou, K. J. Cell. Sci. (2002) [Pubmed]
  32. Epidermal growth factor inhibits amiloride-sensitive sodium absorption in renal collecting duct cells. Shen, J.P., Cotton, C.U. Am. J. Physiol. Renal Physiol. (2003) [Pubmed]
  33. Nystatin induces secretion of interleukin (IL)-1beta, IL-8, and tumor necrosis factor alpha by a toll-like receptor-dependent mechanism. Razonable, R.R., Henault, M., Watson, H.L., Paya, C.V. Antimicrob. Agents Chemother. (2005) [Pubmed]
  34. Isolation and characterization of temperature-sensitive mutations in the RAS2 and CYR1 genes of Saccharomyces cerevisiae. Mitsuzawa, H., Uno, I., Oshima, T., Ishikawa, T. Genetics (1989) [Pubmed]
  35. Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis. Skaggs, B.A., Alexander, J.F., Pierson, C.A., Schweitzer, K.S., Chun, K.T., Koegel, C., Barbuch, R., Bard, M. Gene (1996) [Pubmed]
  36. Angiotensin II depolarizes podocytes in the intact glomerulus of the Rat. Gloy, J., Henger, A., Fischer, K.G., Nitschke, R., Mundel, P., Bleich, M., Schollmeyer, P., Greger, R., Pavenstädt, H. J. Clin. Invest. (1997) [Pubmed]
  37. Sodium-dependent nucleoside transport in mouse leukemia L1210 cells. Dagnino, L., Bennett, L.L., Paterson, A.R. J. Biol. Chem. (1991) [Pubmed]
  38. Site-specific mutagenesis and domain substitutions in the loading module of the nystatin polyketide synthase, and their effects on nystatin biosynthesis in Streptomyces noursei. Brautaset, T., Borgos, S.E., Sletta, H., Ellingsen, T.E., Zotchev, S.B. J. Biol. Chem. (2003) [Pubmed]
  39. Cell volume and osmotic properties of erythrocytes after complement lysis measured by flow cytometry. Bauer, J., Valet, G. J. Immunol. (1983) [Pubmed]
  40. Nystatin as a probe for investigating the electrical properties of a tight epithelium. Lewis, S.A., Eaton, D.C., Clausen, C., Diamond, J.M. J. Gen. Physiol. (1977) [Pubmed]
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