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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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HOF1  -  Hof1p

Saccharomyces cerevisiae S288c

Synonyms: CYK2, Cytokinesis protein 2, Homolog of CDC15 protein 1, YM9973.05, YMR032W
 
 
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High impact information on HOF1

  • SCF(Grr1)-mediated degradation of Hof1 may thus represent a novel mechanism to couple exit from mitosis with initiation of cytokinesis [1].
  • While Hof1 is required early in mitosis to assemble a functional actomyosin ring, it is specifically degraded late in mitosis and remains unstable during the entire G1 phase of the cell cycle [1].
  • During cytokinesis, however, the Cyk2 double ring merges with the actomyosin ring and exhibits a contraction-like size change that is dependent on Myo1p [2].
  • Dual function of Cyk2, a cdc15/PSTPIP family protein, in regulating actomyosin ring dynamics and septin distribution [2].
  • These observations suggest that the septin-containing ring is dynamically distinct from the actomyosin ring and that Cyk2p transits between the two types of structures [2].
 

Biological context of HOF1

  • Around telophase, Hof1p is phosphorylated and the double rings merge into a single ring that contracts slightly and may colocalize with the actomyosin structure [3].
  • Video microscopy of cells expressing green fluorescent protein (GFP)-tagged Cyk2p revealed that Cyk2p forms a double ring that coincides with the septins through most of the cell cycle [2].
  • Deletion of either cyk3 or hof1 alone results in a mild cytokinetic phenotype [5-7], but deletion of both genes resulted in lethality and a complete cytokinetic block, suggesting overlapping function [4].
  • Our results also show that downregulation of Hof1 at the end of mitosis is necessary to allow efficient contraction of the actomyosin ring and cell separation during cytokinesis [1].
  • Furthermore, we found that, in the absence of actin overexpression, a novel, Hof1-dependent actin belt is formed at the bud neck of anaphase cells [5].
 

Physical interactions of HOF1

 

Regulatory relationships of HOF1

  • Overexpression of Cyk2p also blocks cytokinesis, most likely due to a loss of the septins from the bud neck, indicating that Cyk2p may also play a role in regulating the localization of the septins [2].
 

Other interactions of HOF1

  • Hof1p localization is dependent on septins but not Myo1p [3].
  • Synthetic lethality suggests that Bni1p and Myo1p belong to one functional pathway, whereas Hof1p and Bnr1p belong to another [3].
  • Vrp1p functions in both actomyosin ring-dependent and Hof1p-dependent pathways of cytokinesis [6].
  • These results indicate that Bnr1p directly interacts with Hof1p as well as with profilin to regulate cytoskeletal functions in S. cerevisiae [7].
  • Interaction of Bnr1p with a novel Src homology 3 domain-containing Hof1p. Implication in cytokinesis in Saccharomyces cerevisiae [7].
 

Analytical, diagnostic and therapeutic context of HOF1

References

  1. Degradation of Hof1 by SCF(Grr1) is important for actomyosin contraction during cytokinesis in yeast. Blondel, M., Bach, S., Bamps, S., Dobbelaere, J., Wiget, P., Longaretti, C., Barral, Y., Meijer, L., Peter, M. EMBO J. (2005) [Pubmed]
  2. Dual function of Cyk2, a cdc15/PSTPIP family protein, in regulating actomyosin ring dynamics and septin distribution. Lippincott, J., Li, R. J. Cell Biol. (1998) [Pubmed]
  3. Roles of Hof1p, Bni1p, Bnr1p, and myo1p in cytokinesis in Saccharomyces cerevisiae. Vallen, E.A., Caviston, J., Bi, E. Mol. Biol. Cell (2000) [Pubmed]
  4. Cyk3, a novel SH3-domain protein, affects cytokinesis in yeast. Korinek, W.S., Bi, E., Epp, J.A., Wang, L., Ho, J., Chant, J. Curr. Biol. (2000) [Pubmed]
  5. Dissection of septin actin interactions using actin overexpression in Saccharomyces cerevisiae. Norden, C., Liakopoulos, D., Barral, Y. Mol. Microbiol. (2004) [Pubmed]
  6. Vrp1p functions in both actomyosin ring-dependent and Hof1p-dependent pathways of cytokinesis. Naqvi, S.N., Feng, Q., Boulton, V.J., Zahn, R., Munn, A.L. Traffic (2001) [Pubmed]
  7. Interaction of Bnr1p with a novel Src homology 3 domain-containing Hof1p. Implication in cytokinesis in Saccharomyces cerevisiae. Kamei, T., Tanaka, K., Hihara, T., Umikawa, M., Imamura, H., Kikyo, M., Ozaki, K., Takai, Y. J. Biol. Chem. (1998) [Pubmed]
 
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