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Gene Review

CDC15  -  Cdc15p

Saccharomyces cerevisiae S288c

Synonyms: Cell division control protein 15, LYT1, YAR019C
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High impact information on CDC15

  • We find that daughter cells activate an Antagonist of MEN pathway (AMEN) in part through induction of the Amn1 protein that binds directly to Tem1 and prevents its association with its target kinase Cdc15 [1].
  • Apical polarization was delayed in cdc15 mutants as compared with budding in control cells and this delay was abolished in a septin mutant [2].
  • We report here that lyt1 is an allele of cdc15, a gene which encodes a protein kinase that functions late in the cell cycle [2].
  • To learn more about the biochemical composition and activity of this ring, we have characterized the in vivo distribution and function of Cyk2p, a budding yeast protein that exhibits significant sequence similarity to the cdc15/PSTPIP family of cleavage furrow proteins [3].
  • However, in cells arrested in late mitosis (M) by a cdc15 mutation, the unique DNA appeared decondensed while the repetitive rDNA region appeared condensed, suggesting that the condensation state of separate regions of the genome may be regulated differently [4].

Biological context of CDC15


Anatomical context of CDC15


Associations of CDC15 with chemical compounds


Physical interactions of CDC15

  • Thus, in nud1-2 cells the failure of Tem1p to interact with Cdc15p at the SPB probably prevents mitotic exit [16].
  • As a consequence, Cdc15 binds to the daughter pole and triggers cytokinesis [9].

Regulatory relationships of CDC15

  • An extra copy of CDC14 suppressed the temperature sensitivity of cdc15-rlt1 cells, but not that of cdc15-1 cells [17].
  • Dominant mutant alleles of yeast protein kinase gene CDC15 suppress the lte1 defect in termination of M phase and genetically interact with CDC14 [17].
  • Thus, Cdc15 promotes the exit from mitosis by directly switching on the kinase activity of Dbf2 [18].
  • The defect of TEM1 was suppressed by a high dose of CDC15, which encodes a protein kinase homologous to mitogen-activated protein kinase kinase kinases [19].

Other interactions of CDC15

  • Both events depend on the mitotic exit pathway components TEM1 and CDC15 [8].
  • Regulation of the mitotic exit protein kinases Cdc15 and Dbf2 [8].
  • The cdc15 mutations thus isolated were recessive with regard to the temperature-sensitive phenotype and were dominant with respect to suppression of lte1 [17].
  • At the end of telophase, a portion of Cdc15p is located at the mother-bud neck, suggesting a possible role for Cdc15p in cytokinesis [7].
  • Using a Cdc15p fusion protein coupled at its carboxyl terminus to green fluorescent protein (GFP), we found that Cdc15p, like its homologue Cdc7p [8] in fission yeast, localizes to the spindle pole bodies (SPBs) during mitosis [7].

Analytical, diagnostic and therapeutic context of CDC15


  1. Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Wang, Y., Shirogane, T., Liu, D., Harper, J.W., Elledge, S.J. Cell (2003) [Pubmed]
  2. Morphogenesis beyond cytokinetic arrest in Saccharomyces cerevisiae. Jiménez, J., Cid, V.J., Cenamor, R., Yuste, M., Molero, G., Nombela, C., Sánchez, M. J. Cell Biol. (1998) [Pubmed]
  3. Dual function of Cyk2, a cdc15/PSTPIP family protein, in regulating actomyosin ring dynamics and septin distribution. Lippincott, J., Li, R. J. Cell Biol. (1998) [Pubmed]
  4. Chromosome condensation and sister chromatid pairing in budding yeast. Guacci, V., Hogan, E., Koshland, D. J. Cell Biol. (1994) [Pubmed]
  5. Chromosome separation and exit from mitosis in budding yeast: dependence on growth revealed by cAMP-mediated inhibition. Anghileri, P., Branduardi, P., Sternieri, F., Monti, P., Visintin, R., Bevilacqua, A., Alberghina, L., Martegani, E., Baroni, M.D. Exp. Cell Res. (1999) [Pubmed]
  6. The Cdc14 phosphatase is functionally associated with the Dbf2 protein kinase in Saccharomyces cerevisiae. Grandin, N., de Almeida, A., Charbonneau, M. Mol. Gen. Genet. (1998) [Pubmed]
  7. Phosphorylation and spindle pole body localization of the Cdc15p mitotic regulatory protein kinase in budding yeast. Xu, S., Huang, H.K., Kaiser, P., Latterich, M., Hunter, T. Curr. Biol. (2000) [Pubmed]
  8. Regulation of the mitotic exit protein kinases Cdc15 and Dbf2. Visintin, R., Amon, A. Mol. Biol. Cell (2001) [Pubmed]
  9. Asymmetric spindle pole localization of yeast Cdc15 kinase links mitotic exit and cytokinesis. Menssen, R., Neutzner, A., Seufert, W. Curr. Biol. (2001) [Pubmed]
  10. The human formin-binding protein 17 (FBP17) interacts with sorting nexin, SNX2, and is an MLL-fusion partner in acute myelogeneous leukemia. Fuchs, U., Rehkamp, G., Haas, O.A., Slany, R., Kōnig, M., Bojesen, S., Bohle, R.M., Damm-Welk, C., Ludwig, W.D., Harbott, J., Borkhardt, A. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  11. Saccharomyces cerevisiae cdc15 mutants arrested at a late stage in anaphase are rescued by Xenopus cDNAs encoding N-ras or a protein with beta-transducin repeats. Spevak, W., Keiper, B.D., Stratowa, C., Castañón, M.J. Mol. Cell. Biol. (1993) [Pubmed]
  12. Nuclear pore complex antigens delineate nuclear envelope dynamics in vegetative and conjugating Saccharomyces cerevisiae. Copeland, C.S., Snyder, M. Yeast (1993) [Pubmed]
  13. CDC15, an essential cell cycle gene in Saccharomyces cerevisiae, encodes a protein kinase domain. Schweitzer, B., Philippsen, P. Yeast (1991) [Pubmed]
  14. The mitotic exit network Mob1p-Dbf2p kinase complex localizes to the nucleus and regulates passenger protein localization. Stoepel, J., Ottey, M.A., Kurischko, C., Hieter, P., Luca, F.C. Mol. Biol. Cell (2005) [Pubmed]
  15. The protein kinases AtMAP3Kepsilon1 and BnMAP3Kepsilon1 are functional homologues of S. pombe cdc7p and may be involved in cell division. Jouannic, S., Champion, A., Segui-Simarro, J.M., Salimova, E., Picaud, A., Tregear, J., Testillano, P., Risueño, M.C., Simanis, V., Kreis, M., Henry, Y. Plant J. (2001) [Pubmed]
  16. Nud1p links astral microtubule organization and the control of exit from mitosis. Gruneberg, U., Campbell, K., Simpson, C., Grindlay, J., Schiebel, E. EMBO J. (2000) [Pubmed]
  17. Dominant mutant alleles of yeast protein kinase gene CDC15 suppress the lte1 defect in termination of M phase and genetically interact with CDC14. Shirayama, M., Matsui, Y., Toh-e, A. Mol. Gen. Genet. (1996) [Pubmed]
  18. Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Mah, A.S., Jang, J., Deshaies, R.J. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  19. The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase. Shirayama, M., Matsui, Y., Toh-E, A. Mol. Cell. Biol. (1994) [Pubmed]
  20. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and analysis of the CEN1-ADE1-CDC15 region. Steensma, H.Y., Crowley, J.C., Kaback, D.B. Mol. Cell. Biol. (1987) [Pubmed]
  21. Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Spellman, P.T., Sherlock, G., Zhang, M.Q., Iyer, V.R., Anders, K., Eisen, M.B., Brown, P.O., Botstein, D., Futcher, B. Mol. Biol. Cell (1998) [Pubmed]
  22. The Saccharomyces cerevisiae CDC6 gene is transcribed at late mitosis and encodes a ATP/GTPase controlling S phase initiation. Zwerschke, W., Rottjakob, H.W., Küntzel, H. J. Biol. Chem. (1994) [Pubmed]
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