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Gene Review

MYO1  -  myosin 1

Saccharomyces cerevisiae S288c

Synonyms: Myosin-1, Type II myosin, YHR023W
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Disease relevance of MYO1


Psychiatry related information on MYO1

  • This motor activity requires hydrolysis of ATP, does not require cytosolic extracts, is sensitive to protease treatment, and displays an ATP concentration dependence similar to that of members of the myosin family of actin-based motors [2].

High impact information on MYO1


Chemical compound and disease context of MYO1

  • Analysis using this assay further demonstrated that the motor activity of these myosins is required for the polymerization step, and that actin polymerization depends on phosphorylation of myosin motor domain by p21-activated kinases (PAKs), downstream effectors of the small guanosine triphosphatase, Cdc42p [7].

Biological context of MYO1


Anatomical context of MYO1


Associations of MYO1 with chemical compounds


Physical interactions of MYO1

  • Mlc1p is also shown to interact with the class II conventional myosin (Myo1p) [21].
  • The UCS domain protein She4p binds to myosin motor domains and is essential for class I and class V myosin function [22].
  • Protease digestion of mitochondrial outer membrane proteins or saturation of myosin-binding sites on F-actin with the S1 subfragment of skeletal myosin block binding [23].
  • Calmodulin could bind to Myo1p through the IQ domain at the neck [24].
  • Moreover, Rvs167p functionally interacts with the myosin Myo2p [25].

Enzymatic interactions of MYO1

  • In this paper we show that PAK1 has similar substrate specificity as MIHCK when assayed against synthetic substrates and that PAK1 phosphorylates the heavy chain (1 mol of P(i) per mol) and activates Acanthamoeba myosin I as MIHCK does [26].
  • Yeast actin mutants with acidic residues at the N terminus either neutralized (DNEQ) or deleted (delta-DSE) were used to assess the role of N-terminal acidic residues in the interactions of actin with myosin in the contractile cycle [27].

Regulatory relationships of MYO1


Other interactions of MYO1


Analytical, diagnostic and therapeutic context of MYO1


  1. Identification of a 180 kD protein in Escherichia coli related to a yeast heavy-chain myosin. Casaregola, S., Norris, V., Goldberg, M., Holland, I.B. Mol. Microbiol. (1990) [Pubmed]
  2. Actin-dependent mitochondrial motility in mitotic yeast and cell-free systems: identification of a motor activity on the mitochondrial surface. Simon, V.R., Swayne, T.C., Pon, L.A. J. Cell Biol. (1995) [Pubmed]
  3. Vik1 Modulates Microtubule-Kar3 Interactions through a Motor Domain that Lacks an Active Site. Allingham, J.S., Sproul, L.R., Rayment, I., Gilbert, S.P. Cell (2007) [Pubmed]
  4. Regulation of the myosin-directed chaperone UNC-45 by a novel E3/E4-multiubiquitylation complex in C. elegans. Hoppe, T., Cassata, G., Barral, J.M., Springer, W., Hutagalung, A.H., Epstein, H.F., Baumeister, R. Cell (2004) [Pubmed]
  5. Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells. Bobola, N., Jansen, R.P., Shin, T.H., Nasmyth, K. Cell (1996) [Pubmed]
  6. Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins. Jansen, R.P., Dowzer, C., Michaelis, C., Galova, M., Nasmyth, K. Cell (1996) [Pubmed]
  7. Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerization. Lechler, T., Shevchenko, A., Li, R. J. Cell Biol. (2000) [Pubmed]
  8. The yeast MYO1 gene encoding a myosin-like protein required for cell division. Watts, F.Z., Shiels, G., Orr, E. EMBO J. (1987) [Pubmed]
  9. The MYO1 gene from Saccharomyces cerevisiae: its complete nucleotide sequence. Sweeney, F.P., Watts, F.Z., Pocklington, M.J., Orr, E. Nucleic Acids Res. (1990) [Pubmed]
  10. Yeast myosin heavy chain mutant: maintenance of the cell type specific budding pattern and the normal deposition of chitin and cell wall components requires an intact myosin heavy chain gene. Rodriguez, J.R., Paterson, B.M. Cell Motil. Cytoskeleton (1990) [Pubmed]
  11. The yeast type II myosin heavy chain: analysis of its predicted polypeptide sequence. Sweeney, F.P., Pocklington, M.J., Orr, E. J. Muscle Res. Cell. Motil. (1991) [Pubmed]
  12. Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis. Bi, E., Maddox, P., Lew, D.J., Salmon, E.D., McMillan, J.N., Yeh, E., Pringle, J.R. J. Cell Biol. (1998) [Pubmed]
  13. The COOH-terminal domain of Myo2p, a yeast myosin V, has a direct role in secretory vesicle targeting. Schott, D., Ho, J., Pruyne, D., Bretscher, A. J. Cell Biol. (1999) [Pubmed]
  14. A type V myosin (Myo2p) and a Rab-like G-protein (Ypt11p) are required for retention of newly inherited mitochondria in yeast cells during cell division. Boldogh, I.R., Ramcharan, S.L., Yang, H.C., Pon, L.A. Mol. Biol. Cell (2004) [Pubmed]
  15. Type II myosin involved in cytokinesis in the fission yeast, Schizosaccharomyces pombe. May, K.M., Watts, F.Z., Jones, N., Hyams, J.S. Cell Motil. Cytoskeleton (1997) [Pubmed]
  16. The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles. Johnston, G.C., Prendergast, J.A., Singer, R.A. J. Cell Biol. (1991) [Pubmed]
  17. Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V. Terrak, M., Otterbein, L.R., Wu, G., Palecanda, L.A., Lu, R.C., Dominguez, R. Acta Crystallogr. D Biol. Crystallogr. (2002) [Pubmed]
  18. Cloning and characterization of a Dictyostelium myosin I heavy chain kinase activated by Cdc42 and Rac. Lee, S.F., Egelhoff, T.T., Mahasneh, A., Côté, G.P. J. Biol. Chem. (1996) [Pubmed]
  19. Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA. Surks, H.K., Richards, C.T., Mendelsohn, M.E. J. Biol. Chem. (2003) [Pubmed]
  20. Structural dynamics of the actin-myosin interface by site-directed spectroscopy. Korman, V.L., Anderson, S.E., Prochniewicz, E., Titus, M.A., Thomas, D.D. J. Mol. Biol. (2006) [Pubmed]
  21. Yeast myosin light chain, Mlc1p, interacts with both IQGAP and class II myosin to effect cytokinesis. Boyne, J.R., Yosuf, H.M., Bieganowski, P., Brenner, C., Price, C. J. Cell. Sci. (2000) [Pubmed]
  22. The UCS domain protein She4p binds to myosin motor domains and is essential for class I and class V myosin function. Wesche, S., Arnold, M., Jansen, R.P. Curr. Biol. (2003) [Pubmed]
  23. Yeast mitochondria contain ATP-sensitive, reversible actin-binding activity. Lazzarino, D.A., Boldogh, I., Smith, M.G., Rosand, J., Pon, L.A. Mol. Biol. Cell (1994) [Pubmed]
  24. Identification and functional analysis of the gene for type I myosin in fission yeast. Toya, M., Motegi, F., Nakano, K., Mabuchi, I., Yamamoto, M. Genes Cells (2001) [Pubmed]
  25. The yeast Rvs161 and Rvs167 proteins are involved in secretory vesicles targeting the plasma membrane and in cell integrity. Breton, A.M., Schaeffer, J., Aigle, M. Yeast (2001) [Pubmed]
  26. p21-activated kinase has substrate specificity similar to Acanthamoeba myosin I heavy chain kinase and activates Acanthamoeba myosin I. Brzeska, H., Knaus, U.G., Wang, Z.Y., Bokoch, G.M., Korn, E.D. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  27. Mutational analysis of the role of the N terminus of actin in actomyosin interactions. Comparison with other mutant actins and implications for the cross-bridge cycle. Miller, C.J., Wong, W.W., Bobkova, E., Rubenstein, P.A., Reisler, E. Biochemistry (1996) [Pubmed]
  28. The novel adaptor protein, Mti1p, and Vrp1p, a homolog of Wiskott-Aldrich syndrome protein-interacting protein (WIP), may antagonistically regulate type I myosins in Saccharomyces cerevisiae. Mochida, J., Yamamoto, T., Fujimura-Kamada, K., Tanaka, K. Genetics (2002) [Pubmed]
  29. An intact SH3 domain is required for myosin I-induced actin polymerization. Geli, M.I., Lombardi, R., Schmelzl, B., Riezman, H. EMBO J. (2000) [Pubmed]
  30. Tropomyosin and actin isoforms modulate the localization of tropomyosin strands on actin filaments. Lehman, W., Hatch, V., Korman, V., Rosol, M., Thomas, L., Maytum, R., Geeves, M.A., Van Eyk, J.E., Tobacman, L.S., Craig, R. J. Mol. Biol. (2000) [Pubmed]
  31. Split decisions: coordinating cytokinesis in yeast. Wolfe, B.A., Gould, K.L. Trends Cell Biol. (2005) [Pubmed]
  32. Suppression of a myosin defect by a kinesin-related gene. Lillie, S.H., Brown, S.S. Nature (1992) [Pubmed]
  33. Sequential assembly of myosin II, an IQGAP-like protein, and filamentous actin to a ring structure involved in budding yeast cytokinesis. Lippincott, J., Li, R. J. Cell Biol. (1998) [Pubmed]
  34. Roles of type II myosin and a tropomyosin isoform in retrograde actin flow in budding yeast. Huckaba, T.M., Lipkin, T., Pon, L.A. J. Cell Biol. (2006) [Pubmed]
  35. Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton. Goodson, H.V., Anderson, B.L., Warrick, H.M., Pon, L.A., Spudich, J.A. J. Cell Biol. (1996) [Pubmed]
  36. Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. Luo, J., Vallen, E.A., Dravis, C., Tcheperegine, S.E., Drees, B., Bi, E. J. Cell Biol. (2004) [Pubmed]
  37. Cytokinesis depends on the motor domains of myosin-II in fission yeast but not in budding yeast. Lord, M., Laves, E., Pollard, T.D. Mol. Biol. Cell (2005) [Pubmed]
  38. Flow cytometry analysis of cell cycle in myosin II-deficient yeast. Cruz, J.A., Villanueva, L., Rodríguez-Medina, J.R. Puerto Rico health sciences journal. (1998) [Pubmed]
  39. Distribution of actin-binding protein and myosin in macrophages during spreading and phagocytosis. Stendahl, O.I., Hartwig, J.H., Brotschi, E.A., Stossel, T.P. J. Cell Biol. (1980) [Pubmed]
  40. Distinct functions of calmodulin are required for the uptake step of receptor-mediated endocytosis in yeast: the type I myosin Myo5p is one of the calmodulin targets. Geli, M.I., Wesp, A., Riezman, H. EMBO J. (1998) [Pubmed]
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