Disease relevance of Cell Separation

• These plasminogen activator secreting cells were identified as granule neurons by cell separation methods, nuclear morphology, and their ability to bind tetanus toxin and rabbit antiserum against mouse cerebellum (anti-Cbl-1 antiserum) [1].
• CONCLUSIONS: Cell separation in fruit dehiscence requires a specialized cell layer which is nonlignified and capable of autolysis, specified by a myc/bHLH protein encoded by ALC [2].
• A new method for detecting circulating tumor cells that is based on magnetic-activated cell separation (MACS) and nested mutant allele-specific amplification (nested MASA) was evaluated in patients with colorectal cancer using the p53 and K-ras genes as genetic markers [3].
• By contrast, mutation of Neisseria gonorrhoeae ltgC inhibited cell separation without affecting peptidoglycan monomer production [4].
• An autolysin ring associated with cell separation of Staphylococcus aureus [5].

High impact information on Cell Separation

• We provide evidence that Hh signaling controls A/P cell segregation not by directly modifying structural components but by a Cubitus interruptus (Ci)-mediated transcriptional response [6].
• We propose that the opposing transcriptional activities of Ci and En control cell segregation at the A/P boundary by regulating a single cell adhesion molecule [6].
• Reversal of heparin action after cell separation [7].
• Here, we report the molecular lesions associated with lin-12 gain-of-function mutations, cell isolation experiments, and genetic studies of an unusual lin-12 allele [8].
• Both these problems are solved by controlling when two specific proteins are destroyed: securin, an inhibitor of chromosome segregation, and cyclin B, which inhibits cell separation (cytokinesis) [9].

Chemical compound and disease context of Cell Separation

• A triazine dye, Cibacron blue 3G-A (CB), is an inhibitor of cell separation of staphylococcal spp. therefore, we examined the effect of CB on growth of gram-positive bacteria other than Staphylococcus [10].
• Bacteriocidal effects and inhibition of cell separation of cinnamic aldehyde on Bacillus cereus [11].

Biological context of Cell Separation

• Here we show that the conserved proteins, MO25-like Pmo25, GC kinase Nak1, Mor2, and Orb6, constitute a morphogenesis network that is important for polarity control and cell separation [12].
• Disruption of myo2 inhibited cell proliferation. myo2Delta cells showed normal punctate distribution of interphase actin, but they produced irregular actin rings and septa and were impaired in cell separation [13].
• In a screen for cell cycle-regulated genes in the yeast Saccharomyces cerevisiae, we have identified a gene, EGT2, which is involved in cell separation in the G1 stage of the cell cycle [14].
• Epitope-tagged Cbk1p localizes to both sides of the bud neck in late anaphase, just prior to cell separation [15].
• We developed a simple assay for the measurement of tissue factor procoagulant activity (TF PCA) in whole blood samples that avoids the need for mononuclear cell isolation [16].

Anatomical context of Cell Separation

• Little is known about the molecular machinery that directs secretory vesicles to the site of cell separation during cytokinesis [17].
• Induction of c-Jun expression is restricted to Müller glial cells, the only cells in the tissue that express glutamine synthetase and maintain the ability to proliferate upon cell separation [18].
• Depolymerization of the actin or microtubule network in cells of the intact retina mimics the effects of cell separation, repressing glutamine synthetase induction by a mechanism that involves induction of c-Jun and inhibition of glucocorticoid receptor transcriptional activity [18].
• With the use of -cAMP/+cAMP activity ratios of cAMP-dependent protein kinase (A-kinase) in fat cell extracts as an index of cellular cAMP concentrations, it is apparent from both the current literature and from data presented in this paper that classical cell isolation procedures yield cells whose behavior is unpredictable from day to day [19].
• Functional studies on lymphocytes in adult human bone marrow. I. Immunoglobulin production in vitro after fractionation on a sucrose gradient and T/non-T cell separation [20].

Associations of Cell Separation with chemical compounds

• During filamentous growth, no cell separation or subsequent beta-glucan exposure occurs, and the pathogen fails to activate Dectin-1 [21].
• Human CD34+ cells were subfractionated into three size classes using counterflow centrifugal elutriation followed by immunoadsorption to polystyrene cell separation devices [22].
• The cell separation method we employed has, as its final step, the purification of marrow cells based on their ability to take up (bright) or exclude (dull) the mitochondrial dye, Rhodamine (Rho)-123 [23].
• Four Sprague-Dawley rats with 17-alpha-ethinyl estradiol (EE)-stimulated LDL-receptor activity and five controls received all three tracers simultaneously 90 minutes before collagenase liver perfusion and metrizamide gradient cell separation [24].
• Tannic acid staining and in situ immunolocalisation revealed that cell isolation was due to the activation of glucan synthase complexes intrinsic to sphincters [25].

Gene context of Cell Separation

• After ring contraction, cortical actin patches congregate at the mother-bud neck, and septum formation and cell separation rapidly ensue [26].
• Furthermore, investigation of the relationship between Fkh2 and a related forkhead protein Fkh1 demonstrates that these proteins act in overlapping pathways to regulate cell morphology and cell separation [27].
• Chitin synthase II is required for normal morphology, septation, and cell separation [28].
• Here, we demonstrate that, in addition to mitotic exit, S. cerevisiae MEN gene MOB1 is required for cytokinesis and cell separation [29].
• Analysis of ace2Delta cells reveals that Ace2p is required for cell separation but not for polarized growth [15].

Analytical, diagnostic and therapeutic context of Cell Separation

• Furthermore, the inhibitory effect of magnetic cell separation on CD14(+) cells was evaluated by measurement of cytokine in the culture supernatant by ELISA when the cells were cultured with or without lipopolysaccharide (LPS) [30].
• METHODS: Taurine was introduced into the sarcoplasm of isolated ventricular myocytes, either during cell isolation or by diffusion from a penetrating micropipette, and the effect on aNai was measured using an ion sensitive microelectrode [31].
• Effects of hypophysectomy and cell isolation on the transport of L-arabinose by adipocytes [32].
• We found that total and reduced glutathione levels are decreased by one-half after cell isolation procedure and further decline by 25% during cell culture for 2 h in normal Williams' E medium [33].
• To evaluate whether the method of cell separation affects the properties of luteal cell subpopulations, we sorted and characterized sheep luteal cells by flow cytometry via methods previously used to investigate luteal cell subtypes from the macaque corpus luteum [34].

References