High impact information on PDS5

• Pds5p regulates the maintenance of sister chromatid cohesion and is sumoylated to promote the dissolution of cohesion [1].
• We report that pds5 mutants establish cohesion, but during mitosis exhibit precocious sister dissociation [1].
• The PDS5 gene (precocious dissociation of sisters) was identified in a genetic screen designed to identify genes important for chromosome structure [2].
• PDS5 is an essential gene and homologues are found from yeast to humans [2].
• Pds5p is required to maintain cohesion at centromere proximal and distal sequences [2].

Biological context of PDS5

• Budding yeast PDS5 is an essential gene in mitosis and is required for chromosome condensation and sister chromatid cohesion [3].
• Mutant analysis and live cell imaging indicate that PDS5 play a role in meiosis II as well [3].
• PDS5 plays an important role at first meiotic prophase [3].
• Pds5p and the cohesin complex are required for sister chromatid cohesion and localize to the same chromosomal loci over the same cell cycle window [1].
• RESULTS: We show here that the yeast homologue of Spo76, called Pds5, is essential for establishing sister chromatid cohesion and maintaining it during metaphase [4].

Anatomical context of PDS5

• In HeLa cells, Pds5 proteins physically interact with cohesin and associate with chromatin in a cohesin-dependent manner [5].

Regulatory relationships of PDS5

• TOP2 suppresses mini-chromosome loss in pds5 mutants indicating that it rescues a chromosome segregation defect [6].

Other interactions of PDS5

• Budding yeast PDS5 plays an important role in meiosis and is required for sister chromatid cohesion [3].
• TOP2 suppression is specific for pds5 mutants as it does not suppress mutants in the cohesin complex [6].
• The loading of Pds5p onto chromosome requires the function of REC8, but the association of Rec8p with chromosome is independent of PDS5 [3].
• These results indicate that EVL-14/PDS-5 and SCC-3 have functions in both mitosis and meiosis [7].

References