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Gene Review

LEM3  -  Lem3p

Saccharomyces cerevisiae S288c

Synonyms: Alkylphosphocholine resistance protein LEM3, BRE3, Brefeldin-A sensitivity protein 3, N0333, ROS3, ...
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High impact information on LEM3

  • Elimination of the APLTs, Dnf1p and Dnf2p, or their noncatalytic beta-subunit, Lem3p, blocked the import of radiolabeled lyso-PtdEtn and resulted in growth inhibition of lyso-PtdEtn auxotrophs [1].
  • LdRos3 belongs to the CDC50/Lem3 family, proposed as likely beta subunits for P4-ATPases [2].
  • The phenotype of LdRos3-defective parasites was identical to that of the LdMT-/-, including a defect in the uptake of 7-nitrobenz-2-oxa-1,3-diazol-4-yl-amino)-phosphatidylserine, generally considered as not affected in Lem3p-deficient yeast [2].
  • These data demonstrate a requirement for Lem3p expression for normal phosphatidylcholine and alkylphosphocholine drug transport across the plasma membrane of yeast [3].
  • This approach identified mutations in a single gene, YNL323W/LEM3, that conferred resistance to alkylphosphocholine drugs and inhibited internalization of NBD-labeled phosphatidylcholine [3].

Biological context of LEM3

  • Thus, phospholipid asymmetry plays an important role in the establishment of cell polarity; the Cdc50p/Lem3p family likely constitute potential subunits specific to unique P-type ATPases of the APT subfamily [4].
  • Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway [5].

Anatomical context of LEM3

  • Acyl chain-labeled NBD-phosphatidylcholine (NBD-PC) has been used to identify three gene products (Lem3p, Dnf1p, and Dnf2p) that are required for normal levels of inward-directed phospholipid transport (flip) across the plasma membrane of yeast [6].

Associations of LEM3 with chemical compounds


Other interactions of LEM3

  • Interestingly, these lem3 mutants exhibited nearly normal levels of NBD-labeled phospholipid internalization across the plasma membrane, suggesting that Lem3p may have other functions in addition to regulation of the putative APLT activity of Dnf1p at the plasma membrane [8].
  • Application of this promoter-dependent disruption of genes (PRODIGE) method to three C. glabrata genes (SLT2, LEM3, and PDR1) yielded desired recombinants at frequencies of 20, 31, and 11%, the latter representing a weakly expressed gene [9].
  • These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively [5].


  1. Uptake and Utilization of Lyso-phosphatidylethanolamine by Saccharomyces cerevisiae. Riekhof, W.R., Voelker, D.R. J. Biol. Chem. (2006) [Pubmed]
  2. Phospholipid translocation and miltefosine potency require both L. donovani miltefosine transporter and the new protein LdRos3 in Leishmania parasites. Pérez-Victoria, F.J., Sánchez-Cañete, M.P., Castanys, S., Gamarro, F. J. Biol. Chem. (2006) [Pubmed]
  3. Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine. Hanson, P.K., Malone, L., Birchmore, J.L., Nichols, J.W. J. Biol. Chem. (2003) [Pubmed]
  4. Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae. Saito, K., Fujimura-Kamada, K., Furuta, N., Kato, U., Umeda, M., Tanaka, K. Mol. Biol. Cell (2004) [Pubmed]
  5. A genetic analysis of glucocorticoid receptor signaling. Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae. Sitcheran, R., Emter, R., Kralli, A., Yamamoto, K.R. Genetics (2000) [Pubmed]
  6. Fluorescent, acyl chain-labeled phosphatidylcholine analogs reveal novel transport pathways across the plasma membrane of yeast. Elvington, S.M., Bu, F., Nichols, J.W. J. Biol. Chem. (2005) [Pubmed]
  7. A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae. Kato, U., Emoto, K., Fredriksson, C., Nakamura, H., Ohta, A., Kobayashi, T., Murakami-Murofushi, K., Kobayashi, T., Umeda, M. J. Biol. Chem. (2002) [Pubmed]
  8. Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast. Noji, T., Yamamoto, T., Saito, K., Fujimura-Kamada, K., Kondo, S., Tanaka, K. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
  9. Promoter-dependent disruption of genes: simple, rapid, and specific PCR-based method with application to three different yeast. Edlind, T.D., Henry, K.W., Vermitsky, J.P., Edlind, M.P., Raj, S., Katiyar, S.K. Curr. Genet. (2005) [Pubmed]
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