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Gene Review

DNF2  -  aminophospholipid-translocating P4-type...

Saccharomyces cerevisiae S288c

Synonyms: Flippase DNF2, Phospholipid-transporting ATPase DNF2, YD8557.01, YDR093W
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Disease relevance of DNF2

  • The weight gain of the HF (high fat diet) (162.58 +/- 6.68 g) was significantly (p < 0.05) greater than that of DNF-1, DNF-2, (high fat diet with DNF of 0.5 and 1.0 g/kg body weight, respectively) and control groups (143.19 +/- 7.33 g, 139.20 +/- 8.36 g, 130.23 +/- 8.02 g, respectively) [1].

High impact information on DNF2

  • Removal of P4 ATPases Dnf1p and Dnf2p from budding yeast abolishes inward translocation of 6-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminocaproyl] (NBD)-labeled PS, PE, and phosphatidylcholine (PC) across the plasma membrane and causes cell surface exposure of endogenous PE [2].
  • Elimination of the APLTs, Dnf1p and Dnf2p, or their noncatalytic beta-subunit, Lem3p, blocked the import of radiolabeled lyso-PtdEtn and resulted in growth inhibition of lyso-PtdEtn auxotrophs [3].
  • Thus, flip of phosphatidylcholine molecules with three different Bodipy fluorophores (Bodipy FL, Bodipy 530, and Bodipy 581) was tested and compared with that of NBD-PC in strains carrying deletions in LEM3, DNF1, and DNF2 [4].
  • Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis [5].
  • Our findings demonstrate a requirement for Dnf1p and Dnf2p in lipid translocation across the yeast plasma membrane [5].

Anatomical context of DNF2


Associations of DNF2 with chemical compounds

  • It was also found that the triacylglycerol (TG) levels decreased significantly (p < 0.05) in the DNF-2 group from that of the HF, but there was no significant (p < 0.05) difference between DNF-1 and DNF-2 [1].

Other interactions of DNF2

  • Acyl chain-labeled NBD-phosphatidylcholine (NBD-PC) has been used to identify three gene products (Lem3p, Dnf1p, and Dnf2p) that are required for normal levels of inward-directed phospholipid transport (flip) across the plasma membrane of yeast [4].

Analytical, diagnostic and therapeutic context of DNF2


  1. Yeast hydrolysate reduces body fat of dietary obese rats. Kim, K.M., Chang, U.J., Kang, D.H., Kim, J.M., Choi, Y.M., Suh, H.J. Phytotherapy research : PTR. (2004) [Pubmed]
  2. Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles. Alder-Baerens, N., Lisman, Q., Luong, L., Pomorski, T., Holthuis, J.C. Mol. Biol. Cell (2006) [Pubmed]
  3. Uptake and Utilization of Lyso-phosphatidylethanolamine by Saccharomyces cerevisiae. Riekhof, W.R., Voelker, D.R. J. Biol. Chem. (2006) [Pubmed]
  4. Fluorescent, acyl chain-labeled phosphatidylcholine analogs reveal novel transport pathways across the plasma membrane of yeast. Elvington, S.M., Bu, F., Nichols, J.W. J. Biol. Chem. (2005) [Pubmed]
  5. Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis. Pomorski, T., Lombardi, R., Riezman, H., Devaux, P.F., van Meer, G., Holthuis, J.C. Mol. Biol. Cell (2003) [Pubmed]
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