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Gene Review: 

DNF2  -  aminophospholipid-translocating P4-type...

Saccharomyces cerevisiae S288c

Synonyms: Flippase DNF2, Phospholipid-transporting ATPase DNF2, YD8557.01, YDR093W
 
 
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Disease relevance of DNF2

  • The weight gain of the HF (high fat diet) (162.58 +/- 6.68 g) was significantly (p < 0.05) greater than that of DNF-1, DNF-2, (high fat diet with DNF of 0.5 and 1.0 g/kg body weight, respectively) and control groups (143.19 +/- 7.33 g, 139.20 +/- 8.36 g, 130.23 +/- 8.02 g, respectively) [1].
 

High impact information on DNF2

  • Removal of P4 ATPases Dnf1p and Dnf2p from budding yeast abolishes inward translocation of 6-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminocaproyl] (NBD)-labeled PS, PE, and phosphatidylcholine (PC) across the plasma membrane and causes cell surface exposure of endogenous PE [2].
  • Elimination of the APLTs, Dnf1p and Dnf2p, or their noncatalytic beta-subunit, Lem3p, blocked the import of radiolabeled lyso-PtdEtn and resulted in growth inhibition of lyso-PtdEtn auxotrophs [3].
  • Thus, flip of phosphatidylcholine molecules with three different Bodipy fluorophores (Bodipy FL, Bodipy 530, and Bodipy 581) was tested and compared with that of NBD-PC in strains carrying deletions in LEM3, DNF1, and DNF2 [4].
  • Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis [5].
  • Our findings demonstrate a requirement for Dnf1p and Dnf2p in lipid translocation across the yeast plasma membrane [5].
 

Anatomical context of DNF2

 

Associations of DNF2 with chemical compounds

  • It was also found that the triacylglycerol (TG) levels decreased significantly (p < 0.05) in the DNF-2 group from that of the HF, but there was no significant (p < 0.05) difference between DNF-1 and DNF-2 [1].
 

Other interactions of DNF2

  • Acyl chain-labeled NBD-phosphatidylcholine (NBD-PC) has been used to identify three gene products (Lem3p, Dnf1p, and Dnf2p) that are required for normal levels of inward-directed phospholipid transport (flip) across the plasma membrane of yeast [4].
 

Analytical, diagnostic and therapeutic context of DNF2

References

  1. Yeast hydrolysate reduces body fat of dietary obese rats. Kim, K.M., Chang, U.J., Kang, D.H., Kim, J.M., Choi, Y.M., Suh, H.J. Phytotherapy research : PTR. (2004) [Pubmed]
  2. Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles. Alder-Baerens, N., Lisman, Q., Luong, L., Pomorski, T., Holthuis, J.C. Mol. Biol. Cell (2006) [Pubmed]
  3. Uptake and Utilization of Lyso-phosphatidylethanolamine by Saccharomyces cerevisiae. Riekhof, W.R., Voelker, D.R. J. Biol. Chem. (2006) [Pubmed]
  4. Fluorescent, acyl chain-labeled phosphatidylcholine analogs reveal novel transport pathways across the plasma membrane of yeast. Elvington, S.M., Bu, F., Nichols, J.W. J. Biol. Chem. (2005) [Pubmed]
  5. Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis. Pomorski, T., Lombardi, R., Riezman, H., Devaux, P.F., van Meer, G., Holthuis, J.C. Mol. Biol. Cell (2003) [Pubmed]
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