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Gene Review

RME1  -  Rme1p

Saccharomyces cerevisiae S288c

Synonyms: YGR044C, Zinc finger protein RME1
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High impact information on RME1


Biological context of RME1

  • We find that mutations and progressive deletions in all three zinc fingers can be rescued by fusion of RME1 to the DNA binding domain of another protein [4].
  • RME1 is unusual among yeast repressors in two respects: it acts over a considerable distance (2 kbp) and it can activate transcription from a binding site separated from its natural flanking region [5].
  • The mutation permits sporulation of a/a diploids, which express RME1 from its natural promoter, and of a/alpha diploids constructed to express RME1 from the GAL1 promoter [5].
  • One rare mutant was defective in RME1-dependent repression of an artificial reporter gene as well as the native IME1 gene [5].
  • We have analysed in detail the timing of RME1 transcription at the end of mitosis and in G1, and the roles of the transcription factors Ace2 and Swi5 in mediating this expression [6].

Associations of RME1 with chemical compounds

  • Substitution of serine for cysteine in each of the putative fingers abolishes RME1 function; serine substitutions in the second and third putative fingers do not affect RME1 stability [7].
  • To determine DNA binding domain of Rme1p, a series of Rme1p derivatives fused with maltose-binding protein were purified and characterized by gel mobility shift assay [8].

Physical interactions of RME1

  • We show that Rme1p binds to two sites in the IME1 upstream region, near nt -1950 and -2030 [9].
  • Here, in vivo footprinting studies indicate that Rme1p acts over this distance by preventing the binding of the CYC1 transcriptional activators to UAS1 and UAS2 [10].

Regulatory relationships of RME1

  • In addition, Rme1 is assumed to repress IME1 expression [11].

Other interactions of RME1


Analytical, diagnostic and therapeutic context of RME1

  • Northern blot analysis revealed that transcription of the a-specific gene STE2 and the haploid-specific locus RME1 in these transformants is repressed to the same level as in wild-type MATa/MAT alpha cells [14].
  • Dissection of the DNA binding domain of yeast Zn-finger protein Rme1p, a repressor of meiotic activator IME1 [15].


  1. Quantitative trait loci mapped to single-nucleotide resolution in yeast. Deutschbauer, A.M., Davis, R.W. Nat. Genet. (2005) [Pubmed]
  2. IME1, a positive regulator gene of meiosis in S. cerevisiae. Kassir, Y., Granot, D., Simchen, G. Cell (1988) [Pubmed]
  3. Repression by the yeast meiotic inhibitor RME1. Covitz, P.A., Mitchell, A.P. Genes Dev. (1993) [Pubmed]
  4. Repression and activation domains of RME1p structurally overlap, but differ in genetic requirements. Blumental-Perry, A., Li, W., Simchen, G., Mitchell, A.P. Mol. Biol. Cell (2002) [Pubmed]
  5. Requirement for RGR1 and SIN4 in RME1-dependent repression in Saccharomyces cerevisiae. Covitz, P.A., Song, W., Mitchell, A.P. Genetics (1994) [Pubmed]
  6. Rme1, which controls CLN2 expression in Saccharomyces cerevisiae, is a nuclear protein that is cell cycle regulated. Frenz, L.M., Johnson, A.L., Johnston, L.H. Mol. Genet. Genomics (2001) [Pubmed]
  7. The yeast RME1 gene encodes a putative zinc finger protein that is directly repressed by a1-alpha 2. Covitz, P.A., Herskowitz, I., Mitchell, A.P. Genes Dev. (1991) [Pubmed]
  8. A C-terminal segment with properties of alpha-helix is essential for DNA binding and in vivo function of zinc finger protein Rme1p. Shimizu, M., Murase, A., Hara, M., Shindo, H., Mitchell, A.P. J. Biol. Chem. (2001) [Pubmed]
  9. Genomic footprinting of the yeast zinc finger protein Rme1p and its roles in repression of the meiotic activator IME1. Shimizu, M., Li, W., Covitz, P.A., Hara, M., Shindo, H., Mitchell, A.P. Nucleic Acids Res. (1998) [Pubmed]
  10. Transcriptional repression at a distance through exclusion of activator binding in vivo. Shimizu, M., Li, W., Shindo, H., Mitchell, A.P. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  11. The Tup1-Ssn6 general repressor is involved in repression of IME1 encoding a transcriptional activator of meiosis in Saccharomyces cerevisiae. Mizuno, T., Nakazawa, N., Remgsamrarn, P., Kunoh, T., Oshima, Y., Harashima, S. Curr. Genet. (1998) [Pubmed]
  12. rme1 Mutation of Saccharomyces cerevisiae: map position and bypass of mating type locus control of sporulation. Rine, J., Sprague, G.F., Herskowitz, I. Mol. Cell. Biol. (1981) [Pubmed]
  13. AAR1/TUP1 protein, with a structure similar to that of the beta subunit of G proteins, is required for a1-alpha 2 and alpha 2 repression in cell type control of Saccharomyces cerevisiae. Mukai, Y., Harashima, S., Oshima, Y. Mol. Cell. Biol. (1991) [Pubmed]
  14. The role of cysteine residues in the homeodomain protein Mat alpha 2 in mating-type control of Saccharomyces cerevisiae. Mukai, Y., Ohno-Yamashita, Y., Oshima, Y., Harashima, S. Mol. Gen. Genet. (1997) [Pubmed]
  15. Dissection of the DNA binding domain of yeast Zn-finger protein Rme1p, a repressor of meiotic activator IME1. Shimizu, M., Hara, M., Murase, A., Shindo, H., Mitchell, A.P. Nucleic Acids Symp. Ser. (1997) [Pubmed]
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