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Gene Review

MAK3  -  Mak3p

Saccharomyces cerevisiae S288c

Synonyms: L-A virus GAG protein N-acetyltransferase subunit MAK3, Maintenance of killer protein 3, N-alpha-acetyltransferase 30, N-terminal acetyltransferase C complex catalytic subunit MAK3, NAA30, ...
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Disease relevance of MAK3


High impact information on MAK3

  • [NEX] replication depends on MAK3 but is independent of MAK4, MAK6, MAK27 and MKT1 [2].
  • Cellular functions that promote viral replication include the MAK3-encoded N-acetyltransferase whose modification of the gag N terminus is necessary for L-A virus assembly [3].
  • Elimination of VLPs in the original porinless strain by introduction of the mak10 or the mak3 mutation increased the respiratory defect and prolonged its lag phase on nonfermentable carbon sources [4].
  • In yeast, there are at least three NATs: NAT1, MAK3, and NAT3 [5].
  • Localized mutagenesis and evidence for post-transcriptional regulation of MAK3. A putative N-acetyltransferase required for double-stranded RNA virus propagation in Saccharomyces cerevisiae [6].

Biological context of MAK3

  • Among 41 yeast glutamine auxotrophs, complementation analysis defined a single gene, GLN1, on chromosome 16 between MAK3 and MAK6 [7].
  • Based on the estimated molecular weight and amino-acid sequence, The enzyme is different from two other recently identified methionine N(alpha)-acetyltransferases, NAT2 (Kulkarni, M.S. and Sherman, F. (1994) J. Biol. Chem. 269, 13141-13147) and MAK3 (Tercero, J.C. and Wickner, R.B. (1992) J. Biol. Chem. 267, 20277-20281) [8].
  • We cloned the MAK3 gene based on its genetic map position using physically mapped lambda-clones covering nearly all of the yeast genome [6].
  • Although ORF3 alone is sufficient for MAK3 activity when expressed from an expression vector, in its native context an additional 669 base pairs 3' to the ORF and complementary to the gene for a non-histone protein are necessary for expression, but not for normal steady state transcript levels [6].
  • We show that the first 4 amino acids of the L-A gag protein sequence, MLRF, are a portable signal for N-terminal acetylation by MAK3 [9].

Anatomical context of MAK3


Associations of MAK3 with chemical compounds

  • We propose that MAK3 encodes an N-acetyltransferase whose modification of the L-A major coat protein NH2 terminus is essential for viral assembly, and that unassembled coat protein is unstable [1].

Other interactions of MAK3

  • Crude host factor extracts prepared from mak3 or mak10ta mutants also support the reaction as effectively as that from a wild type strain, while a crude extract prepared from a pet18 mutant grown under the nonpermissive conditions is less effective [11].
  • We show herein EGAP is the mammalian/zebrafish homologue of yeast Mak10p, one subunit of the yeast NatC complex, and describe the cloning of its binding partners Mak3 and Mak31 [10].


  1. MAK3 encodes an N-acetyltransferase whose modification of the L-A gag NH2 terminus is necessary for virus particle assembly. Tercero, J.C., Wickner, R.B. J. Biol. Chem. (1992) [Pubmed]
  2. Plasmids controlled exclusion of the K2 killer double-stranded RNA plasmid of yeast. Wickner, R.B. Cell (1980) [Pubmed]
  3. Double-stranded and single-stranded RNA viruses of Saccharomyces cerevisiae. Wickner, R.B. Annu. Rev. Microbiol. (1992) [Pubmed]
  4. Accumulation of viruslike particles in a yeast mutant lacking a mitochondrial pore protein. Dihanich, M., van Tuinen, E., Lambris, J.D., Marshallsay, B. Mol. Cell. Biol. (1989) [Pubmed]
  5. N(alpha)-acetylation and proteolytic activity of the yeast 20 S proteasome. Kimura, Y., Takaoka, M., Tanaka, S., Sassa, H., Tanaka, K., Polevoda, B., Sherman, F., Hirano, H. J. Biol. Chem. (2000) [Pubmed]
  6. Localized mutagenesis and evidence for post-transcriptional regulation of MAK3. A putative N-acetyltransferase required for double-stranded RNA virus propagation in Saccharomyces cerevisiae. Tercero, J.C., Riles, L.E., Wickner, R.B. J. Biol. Chem. (1992) [Pubmed]
  7. The GLN1 locus of Saccharomyces cerevisiae encodes glutamine synthetase. Mitchell, A.P. Genetics (1985) [Pubmed]
  8. A N(alpha)-acetyltransferase selectively transfers an acetyl group to NH2-terminal methionine residues: purification and partial characterization. Lee, F.J., Lin, L.W., Smith, J.A. Biochim. Biophys. Acta (1997) [Pubmed]
  9. Yeast MAK3 N-acetyltransferase recognizes the N-terminal four amino acids of the major coat protein (gag) of the L-A double-stranded RNA virus. Tercero, J.C., Dinman, J.D., Wickner, R.B. J. Bacteriol. (1993) [Pubmed]
  10. Embryonic growth-associated protein is one subunit of a novel N-terminal acetyltransferase complex essential for embryonic vascular development. Wenzlau, J.M., Garl, P.J., Simpson, P., Stenmark, K.R., West, J., Artinger, K.B., Nemenoff, R.A., Weiser-Evans, M.C. Circ. Res. (2006) [Pubmed]
  11. Replicase of L-A virus-like particles of Saccharomyces cerevisiae. In vitro conversion of exogenous L-A and M1 single-stranded RNAs to double-stranded form. Fujimura, T., Wickner, R.B. J. Biol. Chem. (1988) [Pubmed]
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