Disease relevance of recA

• Transcriptional and translational analyses of recA mutant alleles in Pseudomonas aeruginosa [1].
• This report is the first description of a recA-independent, UV-inducible virus DNA damage repair system [2].
• We fused the Pseudomonas aeruginosa recA promoter to a promoterless Vibrio fisheri lux operon [3].

High impact information on recA

• The UV-A induction of UNL-1 DNA damage reactivation was supported in hosts lacking recA gene function [2].
• The region adjacent to the 3' end of the recA gene is indispensable for normal cell division in a rec-2 strain of Pseudomonas aeruginosa when the recA gene is highly expressed [4].
• Thus, the recA defect in the rec-102 mutant is apparently in the interaction between recA and a lexA-like repressor [1].
• The P. aeruginosa recA-containing plasmid pKML3001 complemented each of these pleiotropic characteristics of the lesB908 mutation, supporting the hypothesis that lesB908 is an allele of the P. aeruginosa recA gene [5].
• Characterization of the Pseudomonas aeruginosa recA gene: the Les- phenotype [5].

Biological context of recA

• A chloramphenicol acetyltransferase gene fusion in the 3' end of the recA transcript showed that the recA gene of P. aeruginosa was induced following treatment with a DNA-damaging agent (methyl methanesulfonate) [1].
• R plasmids pMG1, R2, R931 and pMG15 increased the survival of Pseudomonas aeruginosa exposed to ultraviolet radiation (u.v.) in the wild type, and uvr and polA mutants but did not alter the u.v.-response of a recA mutant [6].

Other interactions of recA

• The synthesis of pyocins starts when a mutagen increases the expression of the recA gene and activates the RecA protein, which cleaves the repressor PrtR, liberating the expression of the protein activator gene prtN [7].
• The R plasmid RPL11 reduced u.v.-survival in the wild type, and uvr and polA mutants but did not alter the u.v.-response of a recA host [6].
• The induced production of resident pyocins caused by pyocin AP41 depended on a recA gene function [8].

Analytical, diagnostic and therapeutic context of recA

• Northern blot analyses indicated that the recA gene in P. aeruginosa was transcribed as two distinct mRNAs of approximately 1.2 and 1.4 kilobases (kb) [1].

References