Disease relevance of araC

• A strain of Escherichia coli constructed by Shapiro has a segment of Mu bacteriophage DNA inserted between the araC and lacZ genes [1].
• The ability of indole derivatives to facilitate RNA polymerase transcription of the L-arabinose operon in Escherichia coli was shown to require the catabolite activator protein (CAP) as well as the araC gene product [2].
• araB Gene and nucleotide sequence of the araC gene of Erwinia carotovora [3].
• The araB and araC genes of Salmonella typhimurium have been cloned onto the plasmid pBR322 [4].

High impact information on araC

• Three binding sites for AraC protein are required for autoregulation of araC in Escherichia coli [5].
• This model presents our interpretations of activation and repression of the araBAD operon and the autoregulation of the araC gene [5].
• Selective inactivation of AraC-binding sites on the DNA demonstrated that araO1 and araO2 are required in vivo to produce repression of araC in the presence of arabinose, whereas araI1 and araO2 are required in its absence [5].
• The araC gene encodes a positive regulatory protein required for L-arabinose utilization in Escherichia coli [6].
• A 3-base-pair deletion centered 60 base pairs from the transcriptional initiation site results in a mutant araC promoter that, in the absence of araC protein, reduces transcriptional activity when compared with the wild-type promoter and is unresponsive to various concentrations of intracellular cAMP in vivo [6].

Biological context of araC

• The nucleotide sequence of the Escherichia coli araC gene and flanking regions have been determined from a series of overlapping fragments using the technique of base-specific chemical cleavage of Maxam and Gilbert (1980) [7].
• The translational start-point for the araC gene was deduced from the N-terminal amino acid sequence of the protein, and is located 165 bp from the 5'-end of araC mRNA [7].
• Plasmids which contained a functional araC gene were identified by genetic complementation tests [8].
• We found that the low-affinity site araO2 is essential for araC autoregulation; araO1 and araI1 provide high-affinity AraC-binding sites, which allow cooperative binding at araO2 [5].
• Three binding sites for AraC protein were shown to be required for the autoregulation of araC: araI1, araO1, and araO2 [5].

Anatomical context of araC

• Finally, a DNA fragment containing the lac promoter and ribosome binding site was placed in front the araC gene [9].

Associations of araC with chemical compounds

• The nucleotide sequence of araC gene was confirmed by the partial amino acid sequences of araC protein and its methionine peptides [7].
• We also found that the araC and araBAD promoters share a common site of positive control by the cyclic AMP-binding protein, located 90 bases from the araBAD and 60 bases from the araC transcriptional start points [10].
• Two hydroxylamine-induced araC mutations were isolated repeatedly; each contained a single amino acid substitution [11].

References