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Gene Review:

ybfE - LexA-regulated protein, CopB family

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0673, JW5816

Fernández De Henestrosa, A.R. et al., Rostas, K. et al., Imlay, J.A. et al., Benson, F.E. et al., Cirz, R.T. et al., et al.

Sweetman, Moxon, Bayliss, Fletcher, Morgan, Macrina,

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Disease relevance of ybfE

The nucleotide sequence of a 2224 bp region of the Escherichia coli chromosome that carries the LexA regulated recN gene has been determined [1].

High impact information on ybfE

The Escherichia coli dinI gene is one of the LexA-regulated genes, which are induced upon DNA damage [2].
TSM does not require lexA-regulated SOS functions but is suppressed in cells defective for homologous recombination genes [3].
These searches identified a total of 69 potential LexA-regulated genes/operons with a heterology index of <15 and included all previously characterized LexA-regulated genes [4].
By using this heterology index, together with other search criteria, such as the location of the putative LexA box relative to a gene or to promoter elements, we have performed computational searches of the entire E. coli genome to identify novel LexA-regulated genes [4].
The nucleotide sequence of a 2505 bp region of the Escherichia coli chromosome containing the LexA regulated ruv gene has been determined [5].

Biological context of ybfE

DNA fragments containing this putative promoter region are shown to bind LexA in vitro and to have LexA-regulated promoter activity in vivo [1].
After exposure to H2O2, mutagenesis and filamentation also occurred with the dose response characteristic of SOS induction and mode one killing, but these responses were not dependent on the lexA-regulated umuC mutagenesis or sfiA filamentation functions, respectively [6].
Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes [5].
The mutA phenotype does not require lexA-regulated SOS mutagenesis functions, and appears to be suppressed in cells defective for RecABC-dependent homologous recombination functions [7].
We also examined induced mutation and found that either the inability to derepress the SOS response or the lack of the LexA-regulated polymerase renders S. aureus unable to evolve antibiotic resistance in vitro in response to UV damage [8].

Other interactions of ybfE

No SOS box, characteristic of LexA-regulated promoters, was found in the 5' upstream region of the P. gingivalis recA homolog [9].

Analytical, diagnostic and therapeutic context of ybfE

Semi-quantitative RT-PCR studies confirmed that H. influenzae mounts a LexA-regulated SOS response following DNA assault [10].

References

Nucleotide sequence and LexA regulation of the Escherichia coli recN gene. Rostas, K., Morton, S.J., Picksley, S.M., Lloyd, R.G. Nucleic Acids Res. (1987) [Pubmed]
Physical interactions between DinI and RecA nucleoprotein filament for the regulation of SOS mutagenesis. Yasuda, T., Morimatsu, K., Kato, R., Usukura, J., Takahashi, M., Ohmori, H. EMBO J. (2001) [Pubmed]
DNA polymerase III from Escherichia coli cells expressing mutA mistranslator tRNA is error-prone. Al Mamun, A.A., Marians, K.J., Humayun, M.Z. J. Biol. Chem. (2002) [Pubmed]
Identification of additional genes belonging to the LexA regulon in Escherichia coli. Fernández De Henestrosa, A.R., Ogi, T., Aoyagi, S., Chafin, D., Hayes, J.J., Ohmori, H., Woodgate, R. Mol. Microbiol. (2000) [Pubmed]
Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes. Benson, F.E., Illing, G.T., Sharples, G.J., Lloyd, R.G. Nucleic Acids Res. (1988) [Pubmed]
Mutagenesis and stress responses induced in Escherichia coli by hydrogen peroxide. Imlay, J.A., Linn, S. J. Bacteriol. (1987) [Pubmed]
Mistranslation induced by streptomycin provokes a RecABC/RuvABC-dependent mutator phenotype in Escherichia coli cells. Balashov, S., Humayun, M.Z. J. Mol. Biol. (2002) [Pubmed]
Complete and SOS-Mediated Response of Staphylococcus aureus to the Antibiotic Ciprofloxacin. Cirz, R.T., Jones, M.B., Gingles, N.A., Minogue, T.D., Jarrahi, B., Peterson, S.N., Romesberg, F.E. J. Bacteriol. (2007) [Pubmed]
Nucleotide sequence of the Porphyromonas gingivalis W83 recA homolog and construction of a recA-deficient mutant. Fletcher, H.M., Morgan, R.M., Macrina, F.L. Infect. Immun. (1997) [Pubmed]
Induction of the SOS regulon of Haemophilus influenzae does not affect phase variation rates at tetranucleotide or dinucleotide repeats. Sweetman, W.A., Moxon, E.R., Bayliss, C.D. Microbiology (Reading, Engl.) (2005) [Pubmed]

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