Disease relevance of chbB

• A single clone encoding the celA and celB genes was identified by screening a T. neapolitana genomic library in Escherichia coli [1].
• The complete nucleotide sequences of Ruminococcus albus genes celA and celB coding for endoglucanase A (EGA) and endoglucanase B (EGB), respectively, have been determined [2].
• Zymogram analysis of extracts from Escherichia coli and supernatants of Bacillus subtilis and B. megaterium, including protease-deficient mutants thereof, which express celA, revealed two active proteins, EG-A-L and EG-A-S, with Mrs of 74,000 and 57,000, respectively [3].
• Development of Thermus-Escherichia shuttle vectors and their use for expression of the Clostridium thermocellum celA gene in Thermus thermophilus [4].
• The celA gene was overexpressed in E. coli when the pET23, T7 phage RNA polymerase system was used [5].

High impact information on chbB

• The celA gene encodes a 257-amino-acid protein, while celB encodes a 274-amino-acid protein [1].
• The celA cDNA was 1.95 kb long and had an open reading frame of 1,284 bp, which encoded a polypeptide having 428 amino acid residues [6].
• The nucleotide sequence of a cellulase cDNA (celA) from the rumen fungus Neocallimastix patriciarum and the primary structure of the protein which it encodes were characterized [6].
• Gene deletion and subcloning studies with celA revealed that EGA hydrolysed both CMC and xylan, and did not contain discrete functional domains [2].
• In contrast, celA cloned in pUC18, under the control of lacZp, directed high-level synthesis of EGA in E. coli JM83 [2].

Biological context of chbB

• The celA structural gene consists of an open reading frame of 1095 bp [2].
• The open reading frame for the celA gene is 780 base pairs and encodes a protein of 260 amino acids with a calculated molecular mass of 28.8 kDa [5].

Anatomical context of chbB

• The nucleotide sequence showed that the gene, celA, encoded a multidomain enzyme containing a family 5 catalytic domain and a reiterated sequence that is involved in the association of a multienzyme complex, the cellulosome [7].

Associations of chbB with chemical compounds

• Northern blots of T. neapolitana mRNA revealed that celA and celB are monocistronic messages, and both genes are inducible by cellobiose and are repressed by glucose [1].

References