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MeSH Review

Bacteriophage T7

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Disease relevance of Bacteriophage T7


High impact information on Bacteriophage T7

  • Using hairpin II of U1 small nuclear RNA (U1hpII) or the 3' stem loop of histone mRNA as bait, we could selectively amplify T7 phage that display either the spliceosomal protein U1A or the histone stem loop-binding protein from a lung cDNA phage library containing more than 10(7) independent clones [6].
  • Targeting of pseudocomplementary PNAs to the promoter of the T7 phage RNA polymerase effectively inhibits transcription initiation [7].
  • Genetic analysis of T7 phage defective in gene 2.5 shows that the gene 2.5 protein is essential for T7 DNA replication and growth [8].
  • The recoverin coding sequence was inserted into a pET-11a expression vector under control of the T7 phage promoter [9].
  • For this, we generated T7 phage display libraries of N-terminally and C-terminally truncated FGF-BP1 fragments that were then panned against immobilized FGF-2 [10].

Chemical compound and disease context of Bacteriophage T7

  • A recombinant vaccinia virus, vBGN4 encoding the mature biglycan core protein as a polyhistidine fusion protein under control of the T7 phage promoter was expressed in HT-1080 cells and UMR106 cells [11].
  • A T7 phage library expressing human liver cDNA was screened against immobilized doxorubicin to isolate doxorubicin binding proteins [12].
  • If this pretreatment was performed in the presence of D-mannose, UV-inactivated T7 phages, and pepsin-digested anti-MIAT antibodies, PMNs maintained their phagocytic activity against the MIAT-negative strain [13].
  • Dielectric membrane breakdown induced by the applied voltage pulse caused the micropipet tip to enter the liposome and a small volume (typically 50-500 x 10(-15) L) of fluorescein, YOYO-intercalated T7-phage DNA, 100-nm-diameter unilamellar liposomes, or fluorescent latex spheres could be injected into the intraliposomal compartment [14].
  • A novel fluorescent probe: europium complex hybridized T7 phage [15].

Biological context of Bacteriophage T7


Anatomical context of Bacteriophage T7


Gene context of Bacteriophage T7

  • Screening for hPDIR-binding proteins using a T7 phage display system revealed that alpha1-antitrypsin binds to hPDIR [22].
  • In addition, untreated T7 phage unexpectedly displayed reduced plating efficiency and decreased DNA synthesis in the xth nfo double mutant [23].
  • T7 phages that contain null mutants of gene 2.5 were constructed by homologous recombination [8].
  • We have examined the effect of amino acid substitution(s) in this region on T7 phage growth and on the interaction of the polymerase with thioredoxin [24].
  • DNA polymerase activity in these phage-infected cell extracts is undetectable at assay temperatures of 30 degrees C or 42 degrees C. Upon infection at 30 degrees C, both DNA synthesis in vivo and DNA polymerase activity in cell extracts assayed at 30 degrees C or 42 degrees C approach levels observed using wild-type T7 phage [25].


  1. Genetic analysis of the interaction between bacteriophage T7 DNA polymerase and Escherichia coli thioredoxin. Himawan, J.S., Richardson, C.C. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  2. Autoantibodies to Annexin XI-A and Other Autoantigens in the Diagnosis of Breast Cancer. Fernández-Madrid, F., Tang, N., Alansari, H., Granda, J.L., Tait, L., Amirikia, K.C., Moroianu, M., Wang, X., Karvonen, R.L. Cancer Res. (2004) [Pubmed]
  3. Recombinant decorin glycoforms. Purification and structure. Ramamurthy, P., Hocking, A.M., McQuillan, D.J. J. Biol. Chem. (1996) [Pubmed]
  4. DNA synthesis blocking lesions induced by singlet oxygen are targeted to deoxyguanosines. Ribeiro, D.T., Bourre, F., Sarasin, A., Di Mascio, P., Menck, C.F. Nucleic Acids Res. (1992) [Pubmed]
  5. Retinoblastoma susceptibility gene product pRB activates hypoxia-inducible factor-1 (HIF-1). Budde, A., Schneiderhan-Marra, N., Petersen, G., Brüne, B. Oncogene (2005) [Pubmed]
  6. T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries. Danner, S., Belasco, J.G. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  7. Double duplex invasion by peptide nucleic acid: a general principle for sequence-specific targeting of double-stranded DNA. Lohse, J., Dahl, O., Nielsen, P.E. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  8. Bacteriophage T7 gene 2.5 protein: an essential protein for DNA replication. Kim, Y.T., Richardson, C.C. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  9. Cloning, expression, and crystallization of recoverin, a calcium sensor in vision. Ray, S., Zozulya, S., Niemi, G.A., Flaherty, K.M., Brolley, D., Dizhoor, A.M., McKay, D.B., Hurley, J., Stryer, L. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  10. Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. Xie, B., Tassi, E., Swift, M.R., McDonnell, K., Bowden, E.T., Wang, S., Ueda, Y., Tomita, Y., Riegel, A.T., Wellstein, A. J. Biol. Chem. (2006) [Pubmed]
  11. Eukaryotic expression of recombinant biglycan. Post-translational processing and the importance of secondary structure for biological activity. Hocking, A.M., Strugnell, R.A., Ramamurthy, P., McQuillan, D.J. J. Biol. Chem. (1996) [Pubmed]
  12. Identification of hNopp140 as a binding partner for doxorubicin with a phage display cloning method. Jin, Y., Yu, J., Yu, Y.G. Chem. Biol. (2002) [Pubmed]
  13. Impairment of phagocytosis by the Klebsiella pneumoniae mannose-inhibitable adhesin-T7 receptor. Pruzzo, C., Guzmán, C.A., Calegari, L., Satta, G. Infect. Immun. (1989) [Pubmed]
  14. Electroinjection of colloid particles and biopolymers into single unilamellar liposomes and cells for bioanalytical applications. Karlsson, M., Nolkrantz, K., Davidson, M.J., Strömberg, A., Ryttsén, F., Akerman, B., Orwar, O. Anal. Chem. (2000) [Pubmed]
  15. A novel fluorescent probe: europium complex hybridized T7 phage. Liu, C.M., Jin, Q., Sutton, A., Chen, L. Bioconjug. Chem. (2005) [Pubmed]
  16. Joints formed by RecA protein from oligonucleotides and duplex DNA block initiation and elongation of transcription. Golub, E.I., Ward, D.C., Radding, C.M. Nucleic Acids Res. (1992) [Pubmed]
  17. Overexpression of the Escherichia coli nuo-operon and isolation of the overproduced NADH:ubiquinone oxidoreductase (complex I). Spehr, V., Schlitt, A., Scheide, D., Guénebaut, V., Friedrich, T. Biochemistry (1999) [Pubmed]
  18. Biophysical and biological properties of newly synthesized dioxinocoumarin derivatives. II. Dark and photoinduced effects on T7 phage, yeast and HeLa cells. Csík, G., Rontó, G., Nocentini, S., Averbeck, S., Averbeck, D., Besson, T., Coudert, G., Guillaumet, G. J. Photochem. Photobiol. B, Biol. (1994) [Pubmed]
  19. Expression of human metallothionein-II fusion protein in Escherichia coli. Yamazaki, S., Nakanishi, M., Hamamoto, T., Hirata, H., Ebihara, A., Tokue, A., Kagawa, Y. Biochem. Int. (1992) [Pubmed]
  20. Host-cell reactivation of alkylated T7 bacteriophage. Lane, D., Mamet-Bratley, M.D., Karska-Wysocki, B. Biochim. Biophys. Acta (1979) [Pubmed]
  21. Expression of immunoglobulin genes tandem in eukaryotic cells under the control of T7 bacteriophage RNA polymerase. Deyev, S.M., Lieber, A., Radko, B.V., Polanovsky, O.L. Appl. Biochem. Biotechnol. (1994) [Pubmed]
  22. Different contributions of the three CXXC motifs of human protein-disulfide isomerase-related protein to isomerase activity and oxidative refolding. Horibe, T., Gomi, M., Iguchi, D., Ito, H., Kitamura, Y., Masuoka, T., Tsujimoto, I., Kimura, T., Kikuchi, M. J. Biol. Chem. (2004) [Pubmed]
  23. Development of T7 phage and T7 phage containing apurinic sites in an exonuclease III, endonuclease IV double mutant of Escherichia coli. Sanchez, G., Mamet-Bratley, M.D. Biochem. Cell Biol. (1992) [Pubmed]
  24. Amino acid changes in a unique sequence of bacteriophage T7 DNA polymerase alter the processivity of nucleotide polymerization. Yang, X.M., Richardson, C.C. J. Biol. Chem. (1997) [Pubmed]
  25. A Mutation in the gene-encoding bacteriophage T7 DNA polymerase that renders the phage temperature-sensitive. Kumar, J.K., Kremsdorf, R., Tabor, S., Richardson, C.C. J. Biol. Chem. (2001) [Pubmed]
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