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Gene Review:

gcl - glyoxylate carboligase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0500, JW0495

Chang, Y.Y. et al., Cusa, E. et al., Ashiuchi, M. et al.

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Disease relevance of gcl

Although the gene organization of gcl and orf258 is analogous to that of the ilv gene operons which encode the E. coli AHAS isozyme large and small subunits, Orf258 does not function as a Gcl subunit [1].

High impact information on gcl

Consistent with a grouping of Gcl with the AHAS and pyruvate oxidase enzymes [1].
Gcl contains a quinone binding site as well as binding site for thiamine pyrophosphate and FAD [1].
We report the cloning, genomic location, and DNA sequence of the gene (called gcl) encoding E. coli Gcl and isolation of mutants lacking the enzyme [1].
Glyoxylate carboligase (Gcl) (EC 4.1.1.47) of Escherichia coli catalyzes the condensation of two molecules of glyoxylate to give tartronic semialdehyde, a key intermediate in glyoxylate catabolism [1].
Of the nine genes encoding proteins required for allantoin degradation, only the one encoding glyoxylate carboligase (gcl), the first enzyme of the pathway leading to glycerate, had been identified and mapped at centisome 12 on the chromosome map [2].

Anatomical context of gcl

We found a significant activity of hydroxypyruvate isomerase in Escherichia coli clone cells harboring an E. coli gene (called orf b0508 or gip), which is located downstream of the glyoxylate carboligase gene [3].

Analytical, diagnostic and therapeutic context of gcl

Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase. An enzyme of the acetohydroxy acid synthase-pyruvate oxidase family [1].

References

Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase. An enzyme of the acetohydroxy acid synthase-pyruvate oxidase family. Chang, Y.Y., Wang, A.Y., Cronan, J.E. J. Biol. Chem. (1993) [Pubmed]
Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli. Cusa, E., Obradors, N., Baldomà, L., Badía, J., Aguilar, J. J. Bacteriol. (1999) [Pubmed]
Biochemical evidence that Escherichia coli hyi (orf b0508, gip) gene encodes hydroxypyruvate isomerase. Ashiuchi, M., Misono, H. Biochim. Biophys. Acta (1999) [Pubmed]

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