Disease relevance of pgk

• Characterization of the molecular mechanisms involved in the differential production of erythrose-4-phosphate dehydrogenase, 3-phosphoglycerate kinase and class II fructose-1,6-bisphosphate aldolase in Escherichia coli [1].
• We report the purification and crystallization of phosphoglycerate kinase from Thermus caldophilus (Tca) [2].

High impact information on pgk

• Genes (pgk, fda) encoding the phosphoglycerate kinase and Class II fructose 1,6-bisphosphate aldolase, respectively, of E. coli were identified [3].
• Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli [3].
• Altogether, this shows that when glucose is present in the growth medium expression of the E. coli genes required for its uptake (pts) and its metabolism (gapA and gapB-pgk) are coordinately activated by a mechanism dependent upon the EIIGlc protein [4].
• In most bacteria, the gene for GAPDH is located upstream of the pgk gene encoding 3-phosphoglycerate kinase (PGK) [4].
• Analysis of proteins encoded from these plasmids showed that the pgk gene product is a 40-kDa protein, and that the gene is transcribed from two promoters, one immediately in front of the gene and one in front of an upstream gene coding for a 38-kDa polypeptide of unknown function [5].

Chemical compound and disease context of pgk

• The EIIGlc protein is involved in glucose-mediated activation of Escherichia coli gapA and gapB-pgk transcription [4].
• The pgk gene of Escherichia coli coding for the phosphoglycerate kinase was subcloned from the Carbon and Clarke collection plasmid pLC33-5 [5].

Biological context of pgk

• The position and direction of transcription of the pgk gene was determined by Tn5 insertion mutagenesis [5].
• Since we found that the nucleotide region upstream of the gapB open reading frame is responsible for part of the PGK production, we analyzed gapB promoter activity in vivo by direct measurement of the mRNA levels by reverse transcription [4].

Associations of pgk with chemical compounds

• A structural determinant for glycerate 3-P kinase (pgk) is located near serA [6].

References