Disease relevance of pstB

• Comparison of the organization of the pst operon of B. subtilis with that of E. coli revealed that the gene corresponding to phoU is missing, while there are two genes homologous to pstB in B. subtilis [1].
• A Bacillus subtilis gene cluster similar to the Escherichia coli phosphate-specific transport (pst) operon: evidence for a tandemly arranged pstB gene [1].

High impact information on pstB

• The pstA(=phoT), pstB and phoU genes are situated at 84 minutes on the Escherichia coli genetic map [2].
• Similar to E. coli, mutagenesis of the ATP-binding cassette gene, pstB, resulted in decreased uptake of phosphate [3].
• Three mutant alleles of the pstC gene and one mutant allele of the pstB gene were produced by site-directed mutagenesis [4].
• Deletion plasmids of various sizes were constructed in vitro, and the presence of phoS and phoT genes on the cloned DNA fragments was tested by introducing the plasmids into phoS64 and phoT9 strains for complementation tests [5].
• It was concluded that the three genes pstA, pstB, and phoT were part of an operon and that the phosphate-binding protein was not coded for by any of these genes [6].

Biological context of pstB

• The data indicate that pstB, an essential gene of the PST transport system, is promoting the binding capacity of the Pi-binding protein [7].
• Complementation tests between phoS and phoT mutations showed that they belong to the same cistron [8].
• In contrast to other phoS mutations (which have the same phenotype) it complements the mutations in phoT and pstB [9].

References