Disease relevance of mglB

• The mglB gene of Escherichia coli codes for a galactose-binding protein (GBP) that serves both as the galactose chemoreceptor and as the recognition component of the beta-methylgalactoside transport system [1].
• The mglB gene of Salmonella typhimurium LT2 coding for the galactose-binding protein (GBP) was sequenced [2].

High impact information on mglB

• The nucleotide sequences of the NH2-terminal regions of the mglB (galactose-binding protein) and the araF (arabinose-binding protein) genes have been determined [3].
• Three genes (mglA, mglB, and mglC) required for active transport of substrate by the methylgalactoside permease were identified in a hybrid ColE1-DNA plasmid isolated from a clone (pLC3-14) of the Clarke-Carbon bank of Escherichia coli genes [4].
• The 36,000-dalton protein is the galactose-binding protein previously identified as the mglB product [4].
• Located 106 bp downstream of the putative mglB gene was a 3'-truncated open reading frame with 73.8% similarity and 66.3% identity to mglA of Escherichia coli, suggesting a gene arrangement within an operon which is similar to those of other bacteria [5].
• The operator proximal ORF, mglB, encodes the galactose binding protein, a periplasmic protein of 332 amino acids including the 23 residue amino-terminal signal peptide [6].

Biological context of mglB

• The data indicate that the mgl operon contained three genes, which were transcribed in the order mglB, mglA, and mglC [7].
• The mglB sequence of Salmonella typhimurium LT2; promoter analysis by gene fusions and evidence for a divergently oriented gene coding for the mgl repressor [2].
• T. pallidum mglA and mglC are located downstream of mglB, consistent with the gene order of previously identified mgl operons [8].

Other interactions of mglB

• Our sequence also included part of the mglA gene, which is immediately distal to mglB [1].

Analytical, diagnostic and therapeutic context of mglB

• To investigate the interaction between GBP and Trg, the chemotactic signal transducer for galactose, we sequenced the mglB genes from wild-type and mglB551 mutant strains [1].

References