Disease relevance of psaB

• We have cloned and sequenced the psaA and psaB genes from the unicellular cyanobacterium Synechocystis sp. PCC 6803 [1].

High impact information on psaB

• P(700) is a chlorophyll dimer ligated by the core subunits psaA and psaB [2].
• Fe3+ deficiency decreases rates of photosynthetic electron transport and the mRNA and corresponding protein levels as observed using specific probes. mRNA levels of psaB increased 20-fold during recovery from Fe3+ deficiency, as compared to the control. psaD transcript levels increased to 160% during recovery as compared to the control [3].
• In order to investigate the biogenesis of PS I, the psaB gene was inactivated by targeted mutagenesis in the unicellular cyanobacterium Synechocystis 6803 [4].
• Quantitation of hybridization to northern blots revealed only a slight decrease in the accumulation of the psaA-psaB transcript in cells grown in complete darkness with glucose and in LAHG cells, relative to light-grown cells [1].
• Expression of photosynthesis genes in the cyanobacterium Synechocystis sp. PCC 6803: psaA-psaB and psbA transcripts accumulate in dark-grown cells [1].

Biological context of psaB

• The psaAB operon was transcribed as a dicistronic transcript, which was processed into smaller, putatively monocistronic psaA and psaB transcript species [5].

Analytical, diagnostic and therapeutic context of psaB

• The ability to inactivate psaB makes it a viable target for site-directed mutagenesis [4].

References