Disease relevance of ureA

• Purification of recombinant Helicobacter pylori urease apoenzyme encoded by ureA and ureB [1].
• These data indicate that the ureA and ureB genes encoding H. pylori urease are transcribed and translated in E. coli and that these genes alone are sufficient for the synthesis and assembly of the native size enzyme [1].

High impact information on ureA

• By sequencing the DNA region adjacent to the transposon insertion site two open reading frames, designated ureA and ureB, were identified [2].
• Here, we examine the activity of MutY on the 8-oxoG oxidation products guanidinohydantoin (Gh), two diastereomers of spiroiminodihydantoin (Sp1 and Sp2), oxaluric acid (Oa), and urea (Ur) [3].
• In addition, Fur-like recognition sites are present in three independent O157:H7 isolates upstream of ureD and ureA [4].
• A similar lack of nickel responsiveness was observed upon removal of a 19-bp palindromic sequence in the ureA promoter, as demonstrated by using a genomic ureA::lacZ reporter gene fusion [5].
• Activation of Helicobacter pylori ureA promoter by a hybrid Escherichia coli-H. pylori rpoD gene in E. coli [6].

Biological context of ureA

• SE5000 containing recombinant H. pylori urease genes was grown in minimal medium containing no amino acids, NiCl2 was added to 0.75 microM, and structural genes ureA and ureB (pHP902) were overexpressed in trans to the complete urease gene cluster (pHP808) [7].
• Introduction of the shuttle vector clone DNA (pBHP489K/AB; 6.7 kb), containing the ureA and ureB genes of H. pylori, into urease-negative mutants of H. pylori led to the restoration of their urease activity [8].
• Ribotyping with this probe demonstrated less diversity among isolates from rhesus monkeys than was reported among isolates of H. pylori from humans, as did RFLP analysis of a PCR fragment of the ureA-ureB gene cluster [9].
• The three accessory genes were able to activate apo-urease in vivo when they were cloned into a compatible expression vector and cotransformed into cells carrying the plasmid containing ureA, ureB, and ureC [10].
• We have made mutations in known or potential virulence genes, including ureA, hopZ, and vacA, using kanamycin- and kanamycin/lacZ-marked transposons [11].

Other interactions of ureA

• The structural genes ureA, ureB, and ureC encode the 11-, 12-, and 61-kDa subunits, respectively [12].
• Mutations were obtained, with locations determine by DNA sequencing, in all genes except ureA and ureE [13].
• Putative promoterlike sequences were identified upstream of ureD, ureA, and ureF [14].

Analytical, diagnostic and therapeutic context of ureA

• PCR targeting ure genes (ureA, -B, -C, -D, -E, -F, and -G) demonstrated that each of these genes was present in 58 of 59 EHEC O157:H7 isolates [15].

References