Disease relevance of crp

• The deduced amino acid sequence of ORFX was 53% identical and 87% similar to a divergent ORF of unknown function located 5' to the E. coli crp gene [1].
• Catabolite repression of amylase production and the 'acid toxicity' effect both require crp and were linked to cAMP levels [2].
• Catabolite repression of the propionate catabolic genes in Escherichia coli and Salmonella enterica: evidence for involvement of the cyclic AMP receptor protein [3].

High impact information on crp

• The promoters of the pts operon of Escherichia coli are controlled by the cyclic AMP receptor protein (CRP) complexed with cAMP (CRP.cAMP) [4].
• They were found to overlap the two low-affinity binding sites, CRP2 and CRP3, determined previously for CRP [5].
• Positive co-regulation of the Escherichia coli carnitine pathway cai and fix operons by CRP and the CaiF activator [5].
• Although different cyclic nucleotides can bind to CRP with almost equal affinity, only in the presence of cAMP could wild-type CRP bind to specific DNA sequences [6].
• Molecular genetic studies have identified a class of mutants, CRP*, which either do not require exogenous cAMP for activation or can be activated by cGMP [6].

Chemical compound and disease context of crp

• Cyclic AMP-dependent osmoregulation of crp gene expression in Escherichia coli [7].

Biological context of crp

• Furthermore, cells with defects in cya or crp exhibited reduced transcriptional activation of prpR and prpBCDE in Escherichia coli [3].
• The crp gene was cloned, expressed and inactivated from the JMP636 chromosome [2].
• In this in vitro study, five CRP* mutants, namely, D53H, S62F, G141Q, G141K, and L148R, were tested for their abilities to bind the lac promoter sequence and the effects of cyclic nucleotides in modulating DNA sequence recognition [6].
• A putative sequence similar to the consensus sequence for the E. coli cyclic AMP receptor protein binding site (-312 bp) was found upstream of the start codon [8].

Associations of crp with chemical compounds

• The induction of mdtEF by GlcNAc was also greatly inhibited by the addition of cyclic AMP (cAMP) and completely abolished upon deletion of the cAMP receptor protein gene (crp) [9].
• The recently identified CprK branch of the CRP (cyclic AMP receptor protein)-FNR (fumarate and nitrate reduction regulator) family of transcriptional regulators includes proteins that activate the transcription of genes encoding proteins involved in reductive dehalogenation of chlorinated aromatic compounds [10].

References