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MeSH Review:

Chromosomes, Artificial, P1 Bacteriophage

Poppe, M. et al., Katsuren, K. et al., Maddox, L.O. et al., Mecklenbeck, S. et al., Walter, L. et al., et al.

Kure, Kojima, Kudo, Kanno, Aoki, Suzuki, Shinka, Sakata, Narisawa, Matsubara, Walter, Stark, Helou, Flügge, Levan, Günther,

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High impact information on Chromosomes, Artificial, P1 Bacteriophage

We assembled a P1-derived artificial chromosome (PAC) contig spanning the TRMA candidate region [1].
Recently we constructed a 2.4-Mb, P1-derived artificial chromosome contig spanning the DD candidate region on chromosome 12q23-24 [2].
To investigate the mechanisms controlling OATP expression, the 5'-flanking region of the human OATP gene was isolated from a P1-derived artificial chromosome genomic clone [3].
The entire human COL7A1 locus in a P1-derived artificial chromosome (PAC) was transferred to RDEB-HS keratinocytes by microinjection, after which sustained biosynthesis and secretion of procollagen VII was detected for 1 year in vitro [4].
METHODS: Genomic organization of the gene was determined by comparing the dysferlin cDNA and genomic sequence in P1-derived artificial chromosomes (PACs) containing the gene [5].

Biological context of Chromosomes, Artificial, P1 Bacteriophage

Twenty-one bacterial artificial chromosome (BAC) clones, 32 P1-derived artificial chromosome (PAC) clones, and 2 P1 clones have been isolated using the markers D15S540, GABRB3, GABRA5, GABRG3, D15S822, and D15S217, as well as 34 novel markers developed from the end sequences of BAC/PAC clones [6].
We physically mapped the centromeric part of the BN rat MHC (RT1n haplotype) in a contig of overlapping P1-derived artificial chromosome (PAC) clones encompassing about 300 kb [7].
Following preliminary identification and sequencing of intron 1 by standardized PCR, the application of a suited cDNA/intron primer combination renders a succeeding PCR-mediated screening of cosmid or P1-derived artificial chromosome (PAC) libraries possible, thus identifying genomic clones comprising the searched promoter elements [8].

Gene context of Chromosomes, Artificial, P1 Bacteriophage

We have isolated genomic lambda and P1-derived artificial chromosome clones encoding the human TRHR [9].
To facilitate the mutational and functional analyses of GCSH, we isolated and characterized a human p1-derived artificial chromosome (PAC) clone encoding GCSH [10].
To isolate human ACAT-2 genomic DNA, we designed primers based on the human ACAT-2 cDNA sequence: forward primer 5'-ACACCTCGATCTTGGTCCTGCCATA-3' and reverse primer 5'-GGAATGCAGACAGGGAGTCCT-3'. Using these primers, a human P1-derived artificial chromosome (PAC) library was screened by PCR-based procedures [11].
Using a rat Vps54l-containing P1-derived artificial chromosome (PAC) clone the respective ortholog was mapped to chromosome 11A3 in the mouse [12].
Isolation of a TRbeta PAC (P1 derived artificial chromosome) clone provided the intronic sequences necessary to amplify and sequence the entire TRbeta gene from genomic DNA [13].

References

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A microinjected COL7A1-PAC vector restores synthesis of intact procollagen VII in a dystrophic epidermolysis bullosa keratinocyte cell line. Mecklenbeck, S., Compton, S.H., Mejía, J.E., Cervini, R., Hovnanian, A., Bruckner-Tuderman, L., Barrandon, Y. Hum. Gene Ther. (2002) [Pubmed]
Genomic organization of the dysferlin gene and novel mutations in Miyoshi myopathy. Aoki, M., Liu, J., Richard, I., Bashir, R., Britton, S., Keers, S.M., Oeltjen, J., Brown, H.E., Marchand, S., Bourg, N., Beley, C., McKenna-Yasek, D., Arahata, K., Bohlega, S., Cupler, E., Illa, I., Majneh, I., Barohn, R.J., Urtizberea, J.A., Fardeau, M., Amato, A., Angelini, C., Bushby, K., Beckmann, J.S., Brown, R.H. Neurology (2001) [Pubmed]
Autistic disorder and chromosome 15q11-q13: construction and analysis of a BAC/PAC contig. Maddox, L.O., Menold, M.M., Bass, M.P., Rogala, A.R., Pericak-Vance, M.A., Vance, J.M., Gilbert, J.R. Genomics (1999) [Pubmed]
Physical mapping and evolution of the centromeric class I gene-containing region of the rat MHC. Walter, L., Günther, E. Immunogenetics (2000) [Pubmed]
Use of PCR to screen for promoter elements in genomic DNA library clones. Poppe, M., Hahm, B., Eickelbaum, W., Arand, M., Paweletz, N., Knehr, M. BioTechniques (1999) [Pubmed]
Structural and functional organization of the gene encoding the human thyrotropin-releasing hormone receptor. Matre, V., Høvring, P.I., Orstavik, S., Frengen, E., Rian, E., Velickovic, Z., Murray-McIntosh, R.P., Gautvik, K.M. J. Neurochem. (1999) [Pubmed]
Chromosomal localization, structure, single-nucleotide polymorphisms, and expression of the human H-protein gene of the glycine cleavage system (GCSH), a candidate gene for nonketotic hyperglycinemia. Kure, S., Kojima, K., Kudo, T., Kanno, K., Aoki, Y., Suzuki, Y., Shinka, T., Sakata, Y., Narisawa, K., Matsubara, Y. J. Hum. Genet. (2001) [Pubmed]
Structure of the human acyl-CoA:cholesterol acyltransferase-2 (ACAT-2) gene and its relation to dyslipidemia. Katsuren, K., Tamura, T., Arashiro, R., Takata, K., Matsuura, T., Niikawa, N., Ohta, T. Biochim. Biophys. Acta (2001) [Pubmed]
Identification, characterization and cytogenetic mapping of a yeast Vps54 homolog in rat and mouse. Walter, L., Stark, S., Helou, K., Flügge, P., Levan, G., Günther, E. Gene (2002) [Pubmed]
Five new families with resistance to thyroid hormone not caused by mutations in the thyroid hormone receptor beta gene. Pohlenz, J., Weiss, R.E., Macchia, P.E., Pannain, S., Lau, I.T., Ho, H., Refetoff, S. J. Clin. Endocrinol. Metab. (1999) [Pubmed]

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