Disease relevance of GCSH

• Chromosomal localization, structure, single-nucleotide polymorphisms, and expression of the human H-protein gene of the glycine cleavage system (GCSH), a candidate gene for nonketotic hyperglycinemia [1].

High impact information on GCSH

• Heterozygous GLDC and GCSH gene mutations in transient neonatal hyperglycinemia [2].
• Gene Symbol: GLDC. Disease: NKH glycine encephalopathy [3].
• The interaction between anticancer herbal drug emodin and DNA was studied with differential pulse voltammetry (DPV) and cyclic voltammetry (CV) at the bare or DNA modified GCE and ultraviolet-visible (UV) spectra [4].
• GCSH was expressed in all 29 tissues examined, while T-protein mRNA was detected in 27 of the 29 tissues [1].
• Using the PAC clone as a probe, we mapped GCSH to chromosome 16q24 by fluorescence in situ hybridization [1].

Biological context of GCSH

• GCSH spanned 13.5kb and consisted of five exons [1].
• To facilitate the mutational and functional analyses of GCSH, we isolated and characterized a human p1-derived artificial chromosome (PAC) clone encoding GCSH [1].
• The experiments have clearly proven that OTA interacts and binds to dsDNA strands immobilized onto a GCE surface, but no evidence of DNA-damage caused by OTA was obtained [5].
• Furthermore, acute intravenous administrations of GCE (50-800 mg/kg iv) produced dose-dependent, significant reductions (p < 0.05-0.001) in systemic arterial blood pressure and heart rates of the normotensive and hypertensive rats used [6].

Associations of GCSH with chemical compounds

• NKH is caused by deficiency of the glycine cleavage multi-enzyme system with three specific components encoded by GLDC, AMT, and GCSH [7].
• On the contrary to that observed at a bare GCE, a good repeatability of the amperometric measurements carried out at +900 mV versus Ag/AgCl (R.S.D. of 3.2% for i(p), n=20) was achieved at the CNTs-GCE [8].
• Choline can be covalently grafted on glassy carbon electrodes using cyclic voltammetric method, forming a stable cationic monolayer-modified electrode (Ch/GCE) [9].
• Acute oral administrations of G cupulata aqueous leaf extract (GCE, 50-800 mg/kg po) caused dose-related, significant (p < 0.05-0.001) hypoglycaemia in normal and STZ-treated diabetic rats [6].
• Nitrite reductase (NiR, nitric-oxide: ferricytochrome c oxidoreductase, EC 1.7.2.1) and methyl viologen (MV) were co-immobilized on glassy carbon electrode (GCE, d=3 mm) by polymer entrapment, and the electrode was tested as an electrochemical biosensor for amperometric determination of nitrite [10].

Other interactions of GCSH

• Comprehensive mutation analysis of GLDC, AMT, and GCSH in nonketotic hyperglycinemia [7].

References