Expression of the apoptosis-related genes, caspase-1, caspase-3, DNA fragmentation factor, and apoptotic protease activating factor-1, in human granulosa cells.
OBJECTIVE: To investigate protein and messenger RNA expression products for a subset of apoptosis-related genes in human granulosa cells. DESIGN: In vitro experiment. SETTING: Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts. PATIENT(S): Women undergoing oocyte retrieval for IVF after ovulation induction with gonadotropins. INTERVENTION(S): Granulosa cells were isolated from follicular aspirates after oocyte removal. MAIN OUTCOME MEASURE(S): Reverse transcription polymerase chain reaction and Western blotting for apoptosis-related gene products. RESULT(S): The expression of caspase-1, caspase-3, DNA fragmentation factor, and apoptotic protease activating factor-1, which are intermediate molecules in phylogenetically conserved apoptotic pathways, was demonstrated in granulosa cells from patients undergoing IVF. Moreover, proforms, but not activated enzymes, for both caspase-1 and caspase-3 were observed. CONCLUSION(S): Granulosa cells from patients undergoing IVF have intrinsic apoptotic machinery that could be activated for tissue remodeling.[1]References
- Expression of the apoptosis-related genes, caspase-1, caspase-3, DNA fragmentation factor, and apoptotic protease activating factor-1, in human granulosa cells. Izawa, M., Nguyen, P.H., Kim, H.H., Yeh, J. Fertil. Steril. (1998) [Pubmed]
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