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Chemical Compound Review:

AG-H-34978 3-(6-methoxyquinolin-1- yl)propane-1-sulfonate

Synonyms: ZINC02384880, AR-1E7227, AKOS000814305, FT-0621194, AC1L3XL4, ...

This record was replaced with 123937.

Leblais, V. et al., Ram, S.J. et al., Greco, F.A. et al., Sorscher, E.J. et al., Stern, M. et al., et al.

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Disease relevance of 3-(6-methoxyquinolin-1-yl)propane-1-sulfonic acid

Nasal epithelial cells from non-cystic fibrosis subjects (n = 6) and from cystic fibrosis (CF) patients (delta F508: delta F508, n = 5) were obtained by brushing and loaded with 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ) [1].

High impact information on 3-(6-methoxyquinolin-1-yl)propane-1-sulfonic acid

Anion transport in CFTR transcript antisense-treated cells was then assessed with a halide-specific dye, 6-methoxy-N-(3-sulfopropyl)quinolinium, and fluorescent digital imaging microscopy to monitor halide influx and efflux from single sweat duct cells [2].
We used fluorescence-digital imaging microscopy in combination with a halide-specific fluorescent dye [6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ)] to determine if this defective salt reabsorption is referable to a reduced plasma membrane Cl- permeability of the epithelial cells that line the sweat duct [3].
Cl- conductance was measured in a reconstituted preparation of coated vesicle membrane proteins using the Cl(-)-sensitive fluorescence probe, 6-methoxy-N-(3-sulfopropyl)quinolinium [4].
Cl transport was measured by a stopped-flow quenching assay in endosomes labeled in vivo with the fluorescent Cl indicator 6-methoxy-N-(3-sulfopropyl)quinolinium [5].
A new transport measurement technique has been developed on the basis of the fluorescence quenching by chloride of the dye 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ) [6].

Biological context of 3-(6-methoxyquinolin-1-yl)propane-1-sulfonic acid

COS-7, CFPAC-1, and A549 cells were intranuclearly injected with a Simian virus 40-driven pECE-CFTR plasmid and assayed for halide permeability using the 6-methoxy-N-(3-sulfopropyl)quinolinium fluorescent probe [7].

Anatomical context of 3-(6-methoxyquinolin-1-yl)propane-1-sulfonic acid

The regulation of chloride conductance was investigated in the T84 human colon carcinoma cell line by the quenching of the fluorescent probe 6-methoxy-N-(3-sulfopropyl)quinolinium [8].
We use a fluorescent probe of [Cl-], 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ), to study Cl-/HCO-3 exchange in human erythrocyte ghosts in a stopped-flow apparatus at 4 degrees C. The quench constant of SPQ in our Cl-/HCO-3/HPO=4 system at pH 7.4 is 0.065 +/- 0.005 mM-1 [9].
Chloride-bicarbonate exchange through the human red cell ghost membrane monitored by the fluorescent probe 6-methoxy-N-(3-sulfopropyl)quinolinium [10].

Gene context of 3-(6-methoxyquinolin-1-yl)propane-1-sulfonic acid

CFTR activity was functionally assayed using the 6-methoxy-N-(3-sulfopropyl)quinolinium fluorescent probe and the patch-clamp technique [11].
Anion efflux, assayed by 6-methoxy-N-(3-sulfopropyl)quinolinium fluorescence changes in single cells, was stimulated by VIP or 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate [12].

References

Quantitative fluorescence measurements of chloride secretion in native airway epithelium from CF and non-CF subjects. Stern, M., Munkonge, F.M., Caplen, N.J., Sorgi, F., Huang, L., Geddes, D.M., Alton, E.W. Gene Ther. (1995) [Pubmed]
Antisense oligodeoxynucleotide to the cystic fibrosis gene inhibits anion transport in normal cultured sweat duct cells. Sorscher, E.J., Kirk, K.L., Weaver, M.L., Jilling, T., Blalock, J.E., LeBoeuf, R.D. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
Cl- permeability of human sweat duct cells monitored with fluorescence-digital imaging microscopy: evidence for reduced plasma membrane Cl- permeability in cystic fibrosis. Ram, S.J., Kirk, K.L. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
Modulation of coated vesicle chloride channel activity and acidification by reversible protein kinase A-dependent phosphorylation. Mulberg, A.E., Tulk, B.M., Forgac, M. J. Biol. Chem. (1991) [Pubmed]
Protein kinase A dependent membrane protein phosphorylation and chloride conductance in endosomal vesicles from kidney cortex. Reenstra, W.W., Sabolic, I., Bae, H.R., Verkman, A.S. Biochemistry (1992) [Pubmed]
Membrane chloride transport measured using a chloride-sensitive fluorescent probe. Illsley, N.P., Verkman, A.S. Biochemistry (1987) [Pubmed]
Hyperexpression of recombinant CFTR in heterologous cells alters its physiological properties. Mohammad-Panah, R., Demolombe, S., Riochet, D., Leblais, V., Loussouarn, G., Pollard, H., Baró, I., Escande, D. Am. J. Physiol. (1998) [Pubmed]
Effect of modulation of protein kinase C on the cAMP-dependent chloride conductance in T84 cells. Dechecchi, M.C., Rolfini, R., Tamanini, A., Gamberi, C., Berton, G., Cabrini, G. FEBS Lett. (1992) [Pubmed]
Kinetics of chloride-bicarbonate exchange across the human red blood cell membrane. Greco, F.A., Solomon, A.K. J. Membr. Biol. (1997) [Pubmed]
Chloride-bicarbonate exchange through the human red cell ghost membrane monitored by the fluorescent probe 6-methoxy-N-(3-sulfopropyl)quinolinium. Calafut, T.M., Dix, J.A. Anal. Biochem. (1995) [Pubmed]
beta3-adrenoceptor control the cystic fibrosis transmembrane conductance regulator through a cAMP/protein kinase A-independent pathway. Leblais, V., Demolombe, S., Vallette, G., Langin, D., Baró, I., Escande, D., Gauthier, C. J. Biol. Chem. (1999) [Pubmed]
Salt stress increases abundance and glycosylation of CFTR localized at apical surfaces of salt gland secretory cells. Ernst, S.A., Crawford, K.M., Post, M.A., Cohn, J.A. Am. J. Physiol. (1994) [Pubmed]

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