High impact information on Fast Green FCF

• Histologic analysis of the pancreas with Sirius red (collagen stain) and fast green FCF (noncollagen protein) stains allowed semiquantitative analysis of the ratio between collagen and total protein (C/TP) [1].
• In tissue cultures, fast-green FCF staining coarse granules were found in the cytoplasm of skin fibroblasts from the patient [2].
• A weak (0.01%) fast-green FCF stain changes collagen and sometimes smooth muscle to green, without impairing nucleic acid or mast cell staining [3].
• Fluorescein and FD&C Green No. 3 provided good visual clarity and margins, but required blood-brain barrier (BBB) manipulation [4].
• Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons [5].

Biological context of Fast Green FCF

• Fast Green FCF reduced the frequency, but did not affect the amplitude or kinetics, of miniature synaptic events in a dose-dependent manner [5].
• The technique, which involves a modification of the fast green FCF dye marking procedure, does not interfere with the recording quality of the high-impedance micropipettes, requires no special histology, permits routine production od 5-15 micron diameter marks at extracellular recording sites, and employs a dye known to be noncytotoxic [6].

Anatomical context of Fast Green FCF

• The amphoteric acid dyes acid fuchsin, aniline blue WS, azocarmine G, fast green FCF, light green SF and the wholly acid dye thiazine red R were used for fluorochromy of mast cells in sections of formalin or alcohol-formalin fixed rat tongues [7].

Analytical, diagnostic and therapeutic context of Fast Green FCF

• Recording extracellularly with glass microelectrodes filled with fast green FCF the only cells both ortho- and antidromically activated were localized to the dorsum of the superior vestibular nucleus [8].
• HPLC analysis revealed that eight subsidiary colors existed in commercial Food Green No. 3 (fast green FCF, FD & C Green No. 3). Among them, four subsidiary colors C, F, G, and H were isolated by using preparative HPLC and their structures were determined by MS and NMR [9].

References